Machining Inconel 718 with bio-based nanolubricants can be advantageous in reducing the impact on the overall operating as well as machining expenditure. Added to that, the effective supply of Minimum Quantity Lubricants (MQL) can strengthen and intensify the cooling and lubrication effects of the bio-based nanolubricants. With a proper fluid formulation, the contamination risk of bio-based nanolubricants can be alleviated and their cooling and lubrication ability can be enriched. Up to date, there are limited research works and findings regarding the effectiveness of bio-based nanolubrications for machining of Inconel 718. Hence, in this study, the contrariety of bio-based nanolubricants with different concentration of surfactant content (Coco amido propyl betaine + Sodium dodecyl benzene sulfonate) while machining Inconel 718 with uncoated carbide tool has been explored. The turning parameters were kept constant at 80 m/min cutting speed, 0.1 mm/rev feed rate and 0.1 mm depth of cut, and the effects on tool wear, surface roughness, cutting and spindle power were quantified. Overall, the bio-based nanolubricants with a higher content of surfactant promotes a lower tool wear of 0.509 mm, surface roughness of 1 µm, cutting power of 240 W and spindle power of 5119 W as compared to that of the bio-based nanolubricants with low surfactant content.