Polyhydroxyalkanoates (PHAs) are polyesters generated by numerous microorganism, where it served as a source of energy and carbon store for bacteria. These plastics are biodegradable, eco-friendly and recyclable. In the present study, 2 strains were successfully isolated and identified from molasses wastewater using staining with Nile Red A. Genotypic identification revealed that the isolates belong to species of Pseudomonas alcaliphila and Pseudomonas hydrolytica. Growth curves of both strains were constructed using POLYMATH software showed similar growth patterns and rates. The PHA accumulation of Pseudomonas alcaliphila and Pseudomonas hydrolytica were recorded at 55% and 45% of dry cell weight. The PHA functional group was further determined through Fourier Transfer InfraRed Spectroscopy (FTIR) analysis.
