Mohamad Fahrurrazi Tompang
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Mohamad Fahrurrazi Tompang
Official Name
Mohamad Fahrurrazi, Tompang
Alternative Name
Fahrurrazi Tompang, Mohamad Fahrurrazi
Tompang, Mohamad Fahrurrazi
Main Affiliation
Scopus Author ID
56527099200
Researcher ID
GGZ-1964-2022

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  • Publication
    Isolation and characterization of polyhydroxyalkanoate producing microorganisms from molasses wastewater
    (AIP Publishing, 2023)
    Natasha Yasmin Hasnol Azahari
    ;
    Naresh Sandrasekaran
    ;
    Deiyban Rau
    ;
    Mohamad Fahrurrazi Tompang
    ;
    Kunasundari Balakrishnan
    Polyhydroxyalkanoates (PHAs) are polyesters generated by numerous microorganism, where it served as a source of energy and carbon store for bacteria. These plastics are biodegradable, eco-friendly and recyclable. In the present study, 2 strains were successfully isolated and identified from molasses wastewater using staining with Nile Red A. Genotypic identification revealed that the isolates belong to species of Pseudomonas alcaliphila and Pseudomonas hydrolytica. Growth curves of both strains were constructed using POLYMATH software showed similar growth patterns and rates. The PHA accumulation of Pseudomonas alcaliphila and Pseudomonas hydrolytica were recorded at 55% and 45% of dry cell weight. The PHA functional group was further determined through Fourier Transfer InfraRed Spectroscopy (FTIR) analysis.
  • Publication
    Isolation and characterization of laccase secreting microorganism from Gua Kelam 2, Perlis
    (AIP Publishing, 2023)
    Natasha Yasmin Hasnol Azahari
    ;
    Nor Anis Syafiqah Suhaily
    ;
    Sandrasekaran Naresh
    ;
    Mohamad Fahrurrazi Tompang
    ;
    Kunasundari Balakrishnan
    The current study, involves isolation and characterization of laccase producing microbes from Gua Kelam 2, Perlis, Malaysia. In our study, all isolates were cultivated on nutrient agar containing guaiacol. Only one isolate (GK1) was detected as a positive laccase producer which was then subjected for growth curve and kinetic analysis using nutrient broth and minimal salt medium. For nutrient broth, short exponential phase was recorded from 2nd h until 4th h in contrast to minimal salt medium that exhibited exponential phase from 8th h until 24th h. The highest laccase activity was recorded in nutrient broth (4.50 U L-1) at 4th h incubation compared to minimal salt medium (3.72 U L-1) at 30 h. The isolate GK1, was characterized to be rod shape and Gram positive bacterium. Based on the 16S rRNA sequencing, the isolate determined to be Bacillus subtilis.
  • Publication
    Microbiological removal of hydrogen sulphide from natural rubber latex processing wastewater by Acidithiobacillus thiooxidans strain UniMAP-AIN01
    (IOP Publishing, 2020)
    Sandrasekaran Naresh
    ;
    Nurul Ain Harmiza Abdullah
    ;
    Nor Helya Iman Kamaludin
    ;
    Muhd Afiq Hizami Abdullah
    ;
    Mohamad Fahrurrazi Tompang
    Acidithiobacillus thiooxidans is an acidophilic chemoautotrophic bacterium which capable to convert the toxic hydrogen sulphide in wastewater into non-toxic compounds. The Acidithiobacillus thiooxidans strain UniMAP-AIN01 was previously isolated from a local natural rubber latex processing mill. In this study, the A. thiooxidans strain UniMAP-AIN01 was subjected to its growth performance evaluation in the wastewater of natural rubber latex. The sulphur content in the wastewater was utilized as energy source and support their growth. It was discovered that the A. thiooxidans strain UniMAP-AIN01 has long doubling time of 2 days in thiosulphate medium, while log phase lasted until 6th day of incubation. Regression analysis of the growth kinetic was established with the aid of POLYMATH software. The precision value obtained as follows; linear regression, R2 of 0.9811, adjusted linear regression, Adj R2 of 0.9764, root mean square deviation, RMSD of 0.0015, and variance of 1.992 × 10-5 indicate the data is highly correlated and error is insignificant. Assessment on hydrogen sulphide removal efficiency using locally isolated A. thiooxidans strain UniMAP-AIN01 revealed 90% of hydrogen sulphide removal was achieved within a week.
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