White-rot fungus are known as natural source of antioxidant compounds such as flavonoids, phenolic, and other enzymes through extraction processes. In this study, Phanerochaete chrysosporium and Panus tigrinus biomass were subjected into different extraction conditions to determine the best optimal level for the extraction of phenolic and flavonoids compounds. P. chrysosporium produced high yield of phenolic (0.7041mg/mL) and flavonoids (0.0286mg/mL) compared toP. tigrinus;0.3755mg/mLand0.0109mg/mLof phenolic and flavonoids compounds,respectively.P. chrysosporium has higher antioxidant activity (70.31%) compared with P. tigrinus (25.30%). Therefore, P. chrysosporium was selected to determine the optimum condition for extraction method of phenolic and flavonoids compounds using aquoues-two phase system. The conditions used in the extraction was time within the range of 30min to 180min and temperature range from 250°C to 650°C. The conditions were optimized using Response Surface Method (RSM) under Central Composite Design. The best optimum condition was 105min and 450°C, this condition produced the highest concentrations of phenolic, flavonoids, and antioxidant activity (AA%) at 0.4269 mg/mL, 0.0695 mg/mL, and 76.13% respectively. Thin Layer Chromatography (TLC) was used to determine the presence of phenolic and flavonoids in the sample. The Rfvalue from P. chrysosporium was 0.98 closed to the Rfvalues of gallic acid and 3,4-hydroxybenzoic acid at 0.95 and 0.97,respectively. Fourier Transform Infrared (FTIR) spectroscopy shows that P. chrysosporium have gallic acid, 3,4 hydroxybenzoic acid and small amount of quercetin.
