The Titanium dioxide (TiO2) Nano-particles (NPs) is synthesized via the Sol -gel method by using titanium tetrachloride (TiCl4) as a starting material while varying the applied temperature in the range of (400-900)ᵒC. The main features of the synthesized NPs have been examined by using X - ray diffraction (XRD), Field Emission Electrons Microscopy FESEM and Transmission Electron Microscopy (TEM). The main test of TiO2 NPs was carried out as an antimicrobial and antibacterial agent utilizing four types of bacteria strains (Pseudomonas aeruginosa) and Portus. Results of XRD show that the Titanium dioxide structure demonstrate an Anatase phase at (400 and 450)ᵒC as well as the rutile phase at 900ᵒC. The size of particles is found to be in the range of (15-20) nm for Anatase and for the rutile is in the range of (40-60) nm. FESEM results show the form was crystalline and appeared in the form of cauliflower or clusters for Nanoparticles. The antimicrobial agents can be utilized by the two phases of TiO2 for two types of bacteria (Pseudomonas aeruginosa) and Portus. The obtained results demonstrate that TiO2 in anatase or rutile phases is effective as anti-bacterial agents.
