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Enhanced halophilic lipase secretion by Marinobacter litoralis SW-45 and its potential fatty acid esters release

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cris.author.scopus-author-id 57192171901
cris.author.scopus-author-id 36925239100
cris.author.scopus-author-id 55940833700
cris.author.scopus-author-id 7006558013
cris.author.scopus-author-id 57219159320
dc.contributor.author Musa H.
dc.contributor.author Hafiz Kasim F.
dc.contributor.author Nagoor Gunny A.A.
dc.contributor.author Gopinath S.C.B.
dc.contributor.author Azmier Ahmad M.
dc.date.accessioned 2024-12-14T07:35:07Z
dc.date.available 2024-12-14T07:35:07Z
dc.date.issued 2019-01-01
dc.description.abstract An approach was made to enhance the halophilic lipase secretion by a newly isolated moderate halophilic Marinobacter litoralis SW-45, through the statistical optimization of Plackett-Burman (PB) experimental design and the Face Centered Central Composite Design (FCCCD). Initially, PB statistical design was used to screen the medium components and process parameters, while the One-factor-at-a-time technique was availed to find the optimum level of significant parameters. It was found that MgSO4 · 7H2O, NaCl, agitation speed, FeSO4 · 7H2O, yeast extract and KCl positively influence the halophilic lipase production, whereas temperature, carbon source (maltose), inducer (olive oil), inoculum size, and casein-peptone had a negative effect on enzyme production. The optimum level of halophilic lipase production was obtained at 3.0 g L−1 maltose, 1% (v/v) olive oil, 30 °C growth temperature and 4% inoculum volume (v/v). Further optimization by FCCCD was revealed 1.7 folds improvement in the halophilic lipase production from 0.603 U ml−1 to 1.0307 U ml−1. Functional and biochemical characterizations displayed that the lipase was significantly active and stable in the pH ranges of 7.0–9.5, temperature (30–50 °C), and NaCl concentration (0–21%). The lipase was maximally active at pH 8.0, 12% (w/v) NaCl, and 50 °C temperature. Besides, M. litoralis SW-45 lipase was found to possess the promising industrial potential to be utilized as a biocatalyst for the esterification.
dc.identifier.doi 10.1002/jobm.201800382
dc.identifier.pmid 30270443
dc.identifier.scopus 2-s2.0-85054189393
dc.identifier.uri https://hdl.handle.net/20.500.14170/11000
dc.relation.funding Ministry of Higher Education, Malaysia
dc.relation.grantno undefined
dc.relation.ispartof Journal of Basic Microbiology
dc.relation.ispartofseries Journal of Basic Microbiology
dc.relation.issn 0233111X
dc.rights open access
dc.subject Face Centered Central Composite Design | fatty acid ester | halophilic lipase | Marinobacter litoralis SW-45
dc.title Enhanced halophilic lipase secretion by Marinobacter litoralis SW-45 and its potential fatty acid esters release
dc.type Journal
dspace.entity.type Publication
oaire.citation.endPage 100
oaire.citation.issue 1
oaire.citation.startPage 87
oaire.citation.volume 59
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Sains Malaysia
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person.identifier.orcid 0000-0002-8347-4687
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person.identifier.scopus-author-id 57192171901
person.identifier.scopus-author-id 36925239100
person.identifier.scopus-author-id 55940833700
person.identifier.scopus-author-id 7006558013
person.identifier.scopus-author-id 57219159320
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