Now showing 1 - 3 of 3
  • Publication
    Investigation of the White-rot fungus biomass extraction conditions and their impact on Phenolic, Flavonoids content and antioxidant activities
    ( 2023-07) ;
    Shahizatul Emilia Jahidin
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    ;
    Gidado Muhammad Junaidi
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    ;
    Norhidayah Bohari
    White-rot fungus are known as natural source of antioxidant compounds such as flavonoids, phenolic, and other enzymes through extraction processes. In this study, Phanerochaete chrysosporium and Panus tigrinus biomass were subjected into different extraction conditions to determine the best optimal level for the extraction of phenolic and flavonoids compounds. P. chrysosporium produced high yield of phenolic (0.7041mg/mL) and flavonoids (0.0286mg/mL) compared toP. tigrinus;0.3755mg/mLand0.0109mg/mLof phenolic and flavonoids compounds,respectively.P. chrysosporium has higher antioxidant activity (70.31%) compared with P. tigrinus (25.30%). Therefore, P. chrysosporium was selected to determine the optimum condition for extraction method of phenolic and flavonoids compounds using aquoues-two phase system. The conditions used in the extraction was time within the range of 30min to 180min and temperature range from 250°C to 650°C. The conditions were optimized using Response Surface Method (RSM) under Central Composite Design. The best optimum condition was 105min and 450°C, this condition produced the highest concentrations of phenolic, flavonoids, and antioxidant activity (AA%) at 0.4269 mg/mL, 0.0695 mg/mL, and 76.13% respectively. Thin Layer Chromatography (TLC) was used to determine the presence of phenolic and flavonoids in the sample. The Rfvalue from P. chrysosporium was 0.98 closed to the Rfvalues of gallic acid and 3,4-hydroxybenzoic acid at 0.95 and 0.97,respectively. Fourier Transform Infrared (FTIR) spectroscopy shows that P. chrysosporium have gallic acid, 3,4 hydroxybenzoic acid and small amount of quercetin.
  • Publication
    Methylene blue dye adsorption by using deep eutectic solvent pre-treated wood chips
    ( 2022-11-18)
    Muhammad Masrur Jamil
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    ; ;
    Mohd Faizal Ab Jalil
    ;
    ;
    Mohamed Syazwan Osman
    ;
    Adsorption of Methylene Blue using biomass or bioproduct of industries has received major attention by the researcher and industries. In this study, wood chips treated using deep eutectic solvents (DES) was used for adsorption of methylene blue. The surface and pores of this wood chips were analyzed by using scanning electron microscope (SEM). One Factor At a Time (OFAT) method was carried out to study the effect of contact time and biomass dosage on adsorption efficiency. Evaluation of pretreated wood chips biosorption, isotherms and kinetics were also studied.. Wood chips treated with DES changes the surface area and the pores of wood chips and improved the adsorption process efficiency. Freundlich isotherm and pseudo-second show correlation coefficient of R2, 0.9591 and 0.9999 respectively. Wood chips treated using deep eutectic solvents demonstrate a great potential as a cheap biosorbent for the removal of methylene blue by looking at the isotherm and kinetic studies.
  • Publication
    Methylene blue dye adsorption by using deep eutectic solvent pre-treated wood chips
    ( 2022-11-18)
    Muhammad Masrur Mohd Jamil
    ;
    ; ;
    Mohd Faizal Ab Jalil
    ;
    ;
    Mohamed Syazwan Osman
    ;
    Adsorption of Methylene Blue using biomass or bioproduct of industries has received major attention by the researcher and industries. In this study, wood chips treated using deep eutectic solvents (DES) was used for adsorption of methylene blue. The surface and pores of this wood chips were analyzed by using scanning electron microscope (SEM). One Factor At a Time (OFAT) method was carried out to study the effect of contact time and biomass dosage on adsorption efficiency. Evaluation of pretreated wood chips biosorption, isotherms and kinetics were also studied.. Wood chips treated with DES changes the surface area and the pores of wood chips and improved the adsorption process efficiency. Freundlich isotherm and pseudo-second show correlation coefficient of R2, 0.9591 and 0.9999 respectively. Wood chips treated using deep eutectic solvents demonstrate a great potential as a cheap biosorbent for the removal of methylene blue by looking at the isotherm and kinetic studies.