Che Wan Sharifah Robiah Mohamad
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Preferred name
Che Wan Sharifah Robiah Mohamad
Official Name
Che Wan Sharifah Robiah, Mohamad
Alternative Name
Mohamad, Che Wan Sharifah Robiah
Mohamad, C. W.S.Robiah
Mohamad, Che Wan Syarifah Robiah
Mohamad, C. W.S.R.
Main Affiliation
Scopus Author ID
57194056639
Researcher ID
FNL-7708-2022

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  • Publication
    Antimicrobial activity and phytochemical analysis of Azadirachta Indica (neem) and Clinacanthus Nutans (snake grass) leaves extract
    (AIP Publishing, 2023)
    Che Wan Sharifah Robiah Mohamad
    ;
    Siti Zulaika Zaini
    ;
    Nurul Jannah Jamaluddin
    ;
    Khairul Farihan Kasim
    ;
    Shaida Fariza Sulaiman
    Azadirachta indica (A. indica) and Clinacanthus nutans (C. nutans) is a traditional medicinal plant with a great medical impact on bacteria inflammation. They are few studies for the phytochemical and antimicrobial were conducted for both types of plants. Phytochemical analysis was performed using classical colorimetric methods, which is the folin-Ciocalteu method and aluminum chloride precipitation were employed for quantitative of total phenolic content (TPC) and total flavonoid content (TFC). Meanwhile, antioxidant activity was estimated by DPPH scavenging value. Antimicrobial activity was determined by using the agar well diffusion method against Escherichia coli (E. coli) and Staphylococcus aureus (Staphy. aureus). The methanol crude extract of A. indica and C. nutans showed low content of phenolic, 1.65 mg GAE/g, 0.18 mg GAE/g; flavonoid content is 43.56 mg QE/g and 26.07 mg QE/g respectively for both extracts. Meanwhile, the percentage of scavenging activity is 87.13% and 34.90% for A. indica and C. nutans. Regarding the antimicrobial activity, the A. indica extract inhibits the growth of target bacteria with a minimum inhibitory concentration of 31.25 mg/ml for both strains and almost no inhibitory concentration for C. nutans. Hence, aim this study is to determine two local plant varieties as a source of antimicrobial agents by used soxhlet extraction method.
      11  1
  • Publication
    Radar plot analysis of Helicobacter pylori detection in North Peninsular Malaysia
    (Intisari Sains Medis (Indonesia), 2023)
    Che Wan Sharifah Robiah Mohamad
    ;
    Yahya Mat Arip
    ;
    Uyub Abdul Manaf
    ;
    Marni Azera Markon
    Introduction: Helicobacter pylori (H. pylori) infection is one serious disease that could result in complications such as gastritis, peptic ulcer and gastric cancer. Both invasive and non-invasive methods have been used to detect the infection, however the best detection depends on the clinical setting. One way to determine this is by graphical radar plot analysis, as it provides practical and useful data for many clinical aspects. The aim of this study was to investigate the prevalence rate of H. pylori ureA genotypes infection with a graphical technique. Method: We conducted a cross-sectional study in which data was collected from patients with infection symptoms in north peninsular Malaysia. All eligible subjects were required to perform a simple clinical procedure to provide their cell and saliva samples. Subjects were also examined by the medical staff based on their symptoms. Detection of H.pylori in the samples were conducted with three different detection methods such as culture, biopsy, saliva and the combination of thee three methods. Results: Biopsies were cultured and revealed 19.2% (48/250) of the patients had H. pylori infection. However, when PCR and culture were combined, the prevalence of H. pylori infection jumped by three times to 59.6% (149/250), indicating that PCR on biopsies and saliva was more sensitive. Males who were not Malay and Malay males in the age range of 41 to 60 were found to have significantly different rates of H. pylori infection (p 0.05), according to age group. According to ethnicity, 49.5% (28/58) Malay men were infected compared to 68.1% (62/91) non-Malaysia men (p0.05), while there was no difference between 64.2% (34/53) non-Malaysia women and 51.1% (23/45) Malay women. Conclusion: This study found that saliva method is accurate for the identification of H. pylori. This approach has several advantages that could be helpful to patients worldwide, thus diagnosis and treatment can be done earlier to prevent complications.
      23  1