Nur Hamidah Abdul Halim
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Preferred name
Nur Hamidah Abdul Halim
Official Name
Nur Hamidah, Abdul Halim
Alternative Name
Halim, Nur Hamidah Abdul
Halim, N. H.A.
Hamidah A Halim, N.
Halim, N. H.
Halim, N. Hamidah A.
Abdul Halim, Nur Hamidah
Main Affiliation
Scopus Author ID
55493444600

Research Output

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Fabrication of Graphene Electrode via Graphene Transfer Method for Bisphenol A (BPA) Detection

2021-01-01 , Shukri N.I.B.A. , Norhayati Sabani , Ruslinda A. Rahim , Mohamad Faris Mohamad Fathil , Syarifah Norfaezah Sabki , Nur Hamidah Abdul Halim , Nur Syakimah Ismail

Exposure of BPA is a concern as BPA can seep into food or beverages from containers and can possibly effects on human health especially endocrine systems. An electrochemical-based aptasensor utilizing graphene was developed in detecting endocrine disrupting compound Bisphenol A (BPA, 4,4'-(propane-2,2-diyl) diphenol). The graphene modified electrode was developed via graphene transfer. Fabrication and characterization of graphene transfer was studied in this paper using Scanning Electron Microscopy (SEM) and High-Power Microscope (HPM). In this research, the investigation of interfacial characteristic modified graphene with aptasensor and recognition of BPA with aptasensor had been done using electrochemical impedance spectroscopy (EIS). The increment of charge transfer resistance (Rct) before and after recognition of BPA denoting the accumulation of charge at the electrode surface in this research.

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Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles

2022-10-01 , Jaapar F.N. , Nor Azizah Parmin , Nur Hamidah Abdul Halim , Uda Hashim , Subash Chandra Bose Gopinath , Halim F.S. , Ruslinda A. Rahim , Voon Chun Hong , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Nadzirah S. , Rejali Z. , Afzan A. , Zakaria I.I.

The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.

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Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

2020-12-01 , Nur Nasyifa M.M. , Ruslinda A. Rahim , Nur Hamidah Abdul Halim , Zainol Abidin A.S. , Mohd Faudzi F.N. , Ahmad N.A. , Lockman Z. , Rezek B. , Kromka A. , Subash Chandra Bose Gopinath

Physiological and emotional stress affects the regulation of cortisol secretion, a routine process in circadian rhythm. Regular monitoring of cortisol level as a biomarker in the blood stream becomes vital to determine cortisol-related diseases. This study reports immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor (EGFET) biosensor for cortisol determination in human serum. Solution-processed graphene nanoplatelets were evidenced on the surface by Raman spectroscopy analysis and utilized as the transducing element on the field-effect transistor. Further, confirmed the binding events of the antibody on graphene nanoplatelets using X-Ray Photoelectron Spectroscopy and characterized the electrostatic gating effect of cortisol and intermediate functionalization on graphene nanoplatelets-EGFET. The biosensor exhibited good sensitivity of 72.30 µA.(g/mL)−1 in a linear range between 1.00 pg/mL to 10.00 ng/mL, with a limit of detection (LOD) of 0.85 pg/mL. Confirmation with binding events on the biosensor was done using the relevant molecules, progesterone, cortisone, and corticosterone, and found to be selective towards cortisol. Cortisol was also successfully detected with interference by the human serum, suggesting the capability of graphene nanoplatelets-EGFET sensor for determining cortisol in a complex matrix.

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Micro-interdigitated electrodes genosensor based on Au-deposited nanoparticles for early detection of cervical cancer

2023-12-31 , Jaapar F. Nadhirah , Nor Azizah Parmin , Nur Hamidah Abdul Halim , Uda Hashim , Subash Chandra Bose Gopinath , Fatin Syakirah Halim , Muhammad Nur Aiman Uda , Amilia Afzan , Noorhashimah Mohamad Nor , Khairunisak Abdul Razak

Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.

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Effect of heating power towards synthesis of carbon dots through microwave pyrolysis method for optical-based biosensor

2020-01-08 , Nur Syakimah Ismail , Husain, Umi Shahirah , Selvan S. , Mordani, Nor Afnia , Nurjuliana Juhari , Nur Hamidah Abdul Halim

This study focuses on the effect of heating power in producing carbon dots (CDs) using microwave-assisted pyrolysis from glucose and PEG-200. Glucose is one type of saccharide that contains carbon atom in their structure while PEG-200 is a polymer that acts as a stabilizer. Synthesis of CDs was carried out by using a domestic microwave at different heating power ranging from 300 to 600 Watts. Produced CDs were subjected to dilution, filtering and dialysis procedure for purification purpose. UV-Vis was carried out using PerkinElmer Lambda 35 Spectrophotometer to prove the present of carbon dots in the sample solution. Photoluminescence intensity was observed using Horiba FluoroLog Spectrophotometer together with Horiba iHR550 Imaging Spectrometer to show the emission wavelength and the emission color of CDs prepared. UV-Vis results show the present of CDs in each sample from absorption peaks in the range between 240 nm until 260 nm. The photoluminescence testing shows the emission wavelength of prepared CDs solution is around 430 nm to 570 nm which represent emission color of green-yellow luminescence. From this experiment, CDs that were prepared using a heating power of 450 Watt in 2 minutes and went through the filtering process produce the best luminescence properties. Prepared CDs display electrochemiluminescence (ECL) properties in the presence of co-reactant potassium peroxydisulfate using cyclic voltammetry to pave the way for optical-based biosensor application.

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