Mohd Sharizan Md Sarip
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Preferred name
Mohd Sharizan Md Sarip
Official Name
Mohd Sharizan, Md Sarip
Alternative Name
Md Sarip, Mohd Sharizan
Sarip, M. S.M.
Sharizan Md Sarip, Mohd
Main Affiliation
Scopus Author ID
55387011700
Researcher ID
ABB-6927-2021

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Application of supercritical carbon dioxide extraction of bioactive compounds from cat's whiskers leaves

2025 , Hasmadi Mamat , Azrul Nurfaiz Mohd Faizal , Siti Faridah Mohd Amin , Norliza Julmohammad , Rovina Kobun , Nurul Elyani Mohamad , Mohd Sharizan Md Sarip , Nicky Rahmana Putra , Muhammad Abbas Ahmad Zaini , Ahmad Hazim Abdul Aziz

Cat's whiskers, scientifically known as Orthosiphon stamineus, is a widely used herbal remedy in Malaysia for various health issues. This herb is rich in bioactive compounds, particularly phenolics and flavonoids, which are responsible for its therapeutic properties. To enhance the extraction of these beneficial compounds, the bench-scale supercritical carbon dioxide (SCCO2) extraction method was employed. The study was conducted at various extraction conditions from 10 to 30 MPa of pressure and from 40°C to 80°C of temperature, while keeping the carbon dioxide flow rate, mean particle size of the sample, and extraction duration constant at 10 g/min, 300 Âµm, and 2 h, respectively. The highest extract yield was obtained at optimized condition of 29 MPa and 79°C with 28.12 g/10 g of sample. The highest total phenolic content of approximately 124.324 ppm was obtained at 60°C and 30 MPa, whereas the maximum total flavonoid content reached around 840.595 ppm at 80°C and 30 MPa. This research successfully established the high content of phenolic and flavonoid compounds from cat's whiskers leaves using bench-scale SCCO2 extraction that the extract is applicable for food and pharmaceutical industries.

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Parametric Study on The Rice Bran Protein Extraction Process Using Water as a Solvent

2022-01-01 , Kanapathy M. , Mohd Sharizan Md Sarip , Syahrul Affandi Saidi , Mohd Aminudin Jamlos , Aris N.I.A.

Rice bran is a by-product resulting from the milling process that is frequently underutilized as cattle food or disposed through open-burning despite of its high nutritional and nutraceutical properties. Thus, this research aims in recognizing and exploring rice bran and its extraction methods that could further cultivate in the industry. This study focuses on the rice bran extraction process using water assisted with ultrasonication. The relationship between the operational parameters such as the temperature, extraction time and sample-to-solvent ratio to the protein yield were studied. The rice bran protein was subjected to the surface functional group analysis using Fourier-transform infrared spectroscopy (FTIR). As a conclusion, the extraction temperature of 60°C, sample to solvent ratio of 10 % and extraction time of 25 mins were chosen as the best conditions for the protein extraction. The extraction of the protein from rice bran is highly profitable due to its nutritional and nutraceutical properties as well as it is readily available at low cost.

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Efficient methodology of route selection for driving cycle development

2017-10-29 , A. R. Mahayadin , Shahriman Abu Bakar , Hashim M.S.M. , Zuradzman Mohamad Razlan , Mohd Khairul Faizi Abd Rahman , Harun A. , Nur Saifullah Kamarrudin , I. Ibrahim , Muhammad Akmal Mat Saad , Rani M.F.H. , Zunaidi I. , Sahari M. , Mohd Sharizan Md Sarip , Razali M.Q.H.A.

Driving cycle is a series of data points representing the speed of vehicle versus time and used to determine the performance of vehicle in general. One of the critical portions of driving cycle development is route selection methodology. This paper describes the efficient methodology of route selection for driving cycle development. Previous data from JKR Road Traffic Volume Malaysia (RTVM) in 2015 is studied and analysed to propose the methodology in route selection. The selected routes are then analysed by using Google Maps. For each region, four (4) routes are selected for each urban and rural. For this paper, the selection of route is focused on northern region of Malaysia specifically in Penang. Penang is chosen for this study because it is one of the developed state in Malaysia that has many urban and rural routes. The methods of route selection constructed in this study could be used by other region to develop their own driving cycles.

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Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality

2022-10-01 , Mansor M.R. , Mohd Sharizan Md Sarip , Nik Daud N.M.A. , Saidi S.A. , Mohd Al-Hafiz Mohd Nawi , Mohd Aminudin Jamlos

Rice bran is a by-product of the rice milling process. It contains a high concentration of protein. Rice brans are frequently utilized as feed cattle, fertilizer, and fuel. However, their application as human nutrition supplements is uncommon, and the necessary process for this purpose is yet to be established, including the drying process. This study aims to evaluate the effect of the spray-drying parameters, the inlet temperature, inlet flowrate, and inlet air flowrate, on rice bran protein (RBP) powder and optimize it using response surface methodology (RSM). A thermal water-based extraction method was utilized prior to the drying process. The correlation between the spray-drying parameters, i.e., the inlet temperature (120 to 210 °C), feed flowrate (5 to 55%), and air flowrate (246 to 670 L/h), and the RBP yield were investigated. The quality of the RBP powder was evaluated based on acid amino profiling in the mixture through de novo peptide sequencing. The optimized operating conditions for the maximum yield of RBP powder (25.7 g RBP/100 g RRB) are 178 °C, feed flowrate of 25%, and air flowrate of 450 L/h. The main peptides that contribute to RBP powder protein are globulin and glutelin; meanwhile, prolamin is believed to degrade during the drying process. The process also produced protein sugar, helping to produce fine particles powder without the drying agent.

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Methylene blue dye adsorption by using deep eutectic solvent pre-treated wood chips

2022-11-18 , Muhammad Masrur Jamil , Ahmad Anas Nagoor Gunny , Mohd Sharizan Md Sarip , Mohd Faizal Ab Jalil , Mohamed Mydin Hj M.Abdul Kader , Mohamed Syazwan Osman , Zulkarnain Mohamed Idris

Adsorption of Methylene Blue using biomass or bioproduct of industries has received major attention by the researcher and industries. In this study, wood chips treated using deep eutectic solvents (DES) was used for adsorption of methylene blue. The surface and pores of this wood chips were analyzed by using scanning electron microscope (SEM). One Factor At a Time (OFAT) method was carried out to study the effect of contact time and biomass dosage on adsorption efficiency. Evaluation of pretreated wood chips biosorption, isotherms and kinetics were also studied.. Wood chips treated with DES changes the surface area and the pores of wood chips and improved the adsorption process efficiency. Freundlich isotherm and pseudo-second show correlation coefficient of R2, 0.9591 and 0.9999 respectively. Wood chips treated using deep eutectic solvents demonstrate a great potential as a cheap biosorbent for the removal of methylene blue by looking at the isotherm and kinetic studies.

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Optimization of the rice bran protein powder yield using spray drying technique in response surface methodology

2024-03-21 , Mansor M.R. , Mohd Sharizan Md Sarip , Ibrahim L.H. , Syahrul Affandi Saidi

In this study rice bran protein were extracted and powdered using spray dryer. The optimization of the process were done using Box-Behkenn response surface design. Process parameter such as temperature (120°C,165°C,210°C), aspirator (52%,66%,80%) feed flow rate (5%,30%,55%) and air flow rate (20%,30%55%) were investigated. The result show that the extraction condition have significant effects on extraction yield of protein the obtained experimental data were fitted to a quadratic equation using multiple regression analysis with high coefficient of determination value of 0.8137.an optimization study using Derringer's desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variable for yield (temperature is 120˚C, Aspirator is at 80% feed flowrate set to 5% and air flowrate of 42mmhg) and protein (temperature is 120˚C, aspirator were set at 79.9%, feed flowrate set to 31.8% and air flowrate of 51mmhg) were determined with maximum protein yield of 17.29% per 50 gram of raw rice bran (RRB)

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Quality and stability of rice bran protein powder at different storage condition

2020-12-18 , Mohd Sharizan Md Sarip , Musa N.A. , Syahrul Affandi Saidi , Mansor M.R.

Rice bran contain higher amount of protein, vitamins, essential fatty acids, minerals, dietary fiber and sterol attribute to large food application. The possible application of rice bran protein in food industry are hampered due to the limited information on its stability which may be caused by the rancidity due to the exposure of its oil to lipase. Therefore, stability study for protein extracted form rice bran is essential to evaluate the protein quality at different possible storage condition. Three different storage condition was evaluated; 1. Sample 1 (Store in freezer), 2. Sample 2 (Store in close container in room temperature) and 3. Sample 3 (Store in open container in room temperature). Protein was extracted using hot water process at temperature of 120 C for 20 minutes and dried using spray drying process. All sample are monitored for 5 days for its protein composition, water activity, moisture content and pH. The functional group of the sample where analysed using Fourier transform infrared radiation (FTIR). As a conclusion, the storage condition gives an effect on the water activity and moisture content of the rice bran protein product. However, it not given the significance effect on protein concentration and pH.

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Spray Drying Optimization for Rice Bran Protein (RBP) Powder Using Response Surface Methodology (RSM)

2022-01-01 , Mansor M.R. , Mohd Sharizan Md Sarip , Syahrul Affandi Saidi , Wan Azani Wan Mustafa , Mohd Al-Hafiz Mohd Nawi , Mohd Aminudin Jamlos

Rice bran is a by-product of the rice milling process which contain a high concentration of protein. It’s are often used as a feed cattle, fertilizer, and fuel. Its application as a source of human nutrition is rare due to high lipid concentration. This lipid concentration can be reduced through the extraction process. After the extraction process, the rice bran extract needs to be converted into powder form through a drying process for the quality preservation. In this study, spray drying is utilized as drying technique. The aims of this study were to optimize the spray drying parameter; inlet temperature, feed flowrate and air flowrate for rice bran protein (RBP) powders production. Box Behnken Design (BBD) model in response surface methodology (RSM) are utilized in this study to maximize the RBP powder yield and protein concentration. Raw rice bran (RRB) was extracted using thermal water-based extraction method before the drying process. The optimum condition suggested by the model are at the inlet temperature of 120oC, feed flowrate of 18.38% and air flowrate of 670 L/hr which produced RBP powder yield of 19.42 g RBP/100g RRB and protein concentration of 17.32 mg/ml. The model obtains in this study show a low error between the predicted value and experimental data at 1.68 % and 1.14 % for RBP powder yield and protein concentration respectively. The model can be used to evaluate the process characteristic and understanding.

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β-carotene enrichment in crude palm oil using subcritical water extraction and its relationship with the solubility

2023 , Mohd Sharizan Md Sarip , Noor Azian Morad , Mohd Al-Hafiz Mohd Nawi , Mustafa Kamal Tun Abdul Aziz , Syaripah Za'imah Syed Jaapar

Subcritical water extraction (SWE) was applied for β-carotene enrichment in crude palm oil (CPO) extraction. Investigated SWE operating condition were the pressure of 30, 40 and 50 bar and temperature from 120 to 180 °C. SWE processes were conducted semi-batch for up to 60 min of extraction time. Dynamic method using low solvent rate was used to develop the solubility trend for β-carotene. The pressure and temperature in SWE process gave significant effect to the β-carotene concentration as determined by f value (2, 18) = 158.26, p < 3.78 × 10−12 and f value (3, 18) = 5.91, P < 5.43 × 10−3 respectively. Maximum β-carotene concentration of 857.72 ± 0.34 ppm is obtained at 160 °C and 40 bar. The process was successfully modelled using second order mass transfer coefficient model with r2 between 0.939 and 0.995. Solute solubility cannot be associated directly to extraction efficiency in SWE process for β-carotene enrichment.

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Optimization of Rice Bran Protein Extraction Using Choline Chloride-Glycerol Deep Eutectic Solvent Using Response Surface Methodology (RSM)

2023-01-01 , Kamal Ramlee K.A.F. , Muhammad Nor I.N. , Mohd Sharizan Md Sarip , Mohd Zainudin M.A. , Amirul Ridzuan Abu Bakar , Nawawi M.A. , Nik Muhammad Azhar Nik Daud

A by-product of the rice milling process, rice bran contains four different types of protein: albumin, globulin, prolamin, and glutelin. These proteins are widely known for being hypoallergenic and having nutritional benefits. In order to increase the value of rice bran, the protein was extracted via deep eutectic solvent (DES). Response Surface Methodology (RSM) was used to assess the impact of three operational conditions, including temperature, extraction duration, and the ratio of rice bran to DES, on the yield of rice bran protein after the precipitates were converted into powder form by freeze drying. At optimal working conditions, which were 60 Â°C, 2 h, and a 1:9 ratio of rice bran to DES, the method’s results showed that the highest extracted protein of rice bran was 16.254%. Several techniques, including Fourier transform infrared spectroscopy (FTIR) and Kjeldahl studies, have been used to demonstrate the presence of protein in rice bran powder. In the rice bran protein (RBP), amine (1640.70 cm−1), alcohol (3229.74 cm−1), and alkane (2925.37 cm−1) were all detected using FTIR analysis. Furthermore, Kjeldahl analysis revealed that 15.61% of the rice bran powder’s protein content is present. In conclusion, rice bran’s value as a functional meal can be increased by adding protein through the use of a deep eutectic solvent called green solvent.

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