Mohd Sharizan Md Sarip
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Preferred name
Mohd Sharizan Md Sarip
Official Name
Mohd Sharizan, Md Sarip
Alternative Name
Md Sarip, Mohd Sharizan
Sarip, M. S.M.
Sharizan Md Sarip, Mohd
Main Affiliation
Scopus Author ID
55387011700
Researcher ID
ABB-6927-2021

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Optimization of Rice Bran Protein Extraction Using Choline Chloride-Glycerol Deep Eutectic Solvent Using Response Surface Methodology (RSM)

2023-01-01 , Kamal Ramlee K.A.F. , Muhammad Nor I.N. , Mohd Sharizan Md Sarip , Mohd Zainudin M.A. , Amirul Ridzuan Abu Bakar , Nawawi M.A. , Nik Muhammad Azhar Nik Daud

A by-product of the rice milling process, rice bran contains four different types of protein: albumin, globulin, prolamin, and glutelin. These proteins are widely known for being hypoallergenic and having nutritional benefits. In order to increase the value of rice bran, the protein was extracted via deep eutectic solvent (DES). Response Surface Methodology (RSM) was used to assess the impact of three operational conditions, including temperature, extraction duration, and the ratio of rice bran to DES, on the yield of rice bran protein after the precipitates were converted into powder form by freeze drying. At optimal working conditions, which were 60 Â°C, 2 h, and a 1:9 ratio of rice bran to DES, the method’s results showed that the highest extracted protein of rice bran was 16.254%. Several techniques, including Fourier transform infrared spectroscopy (FTIR) and Kjeldahl studies, have been used to demonstrate the presence of protein in rice bran powder. In the rice bran protein (RBP), amine (1640.70 cm−1), alcohol (3229.74 cm−1), and alkane (2925.37 cm−1) were all detected using FTIR analysis. Furthermore, Kjeldahl analysis revealed that 15.61% of the rice bran powder’s protein content is present. In conclusion, rice bran’s value as a functional meal can be increased by adding protein through the use of a deep eutectic solvent called green solvent.

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Optimization of the rice bran protein powder yield using spray drying technique in response surface methodology

2024-03-21 , Mansor M.R. , Mohd Sharizan Md Sarip , Ibrahim L.H. , Syahrul Affandi Saidi

In this study rice bran protein were extracted and powdered using spray dryer. The optimization of the process were done using Box-Behkenn response surface design. Process parameter such as temperature (120°C,165°C,210°C), aspirator (52%,66%,80%) feed flow rate (5%,30%,55%) and air flow rate (20%,30%55%) were investigated. The result show that the extraction condition have significant effects on extraction yield of protein the obtained experimental data were fitted to a quadratic equation using multiple regression analysis with high coefficient of determination value of 0.8137.an optimization study using Derringer's desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variable for yield (temperature is 120˚C, Aspirator is at 80% feed flowrate set to 5% and air flowrate of 42mmhg) and protein (temperature is 120˚C, aspirator were set at 79.9%, feed flowrate set to 31.8% and air flowrate of 51mmhg) were determined with maximum protein yield of 17.29% per 50 gram of raw rice bran (RRB)

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Effect of Different Drying Process on the Protein-Thiol Content and the Physicochemical Properties of Water-Extracted Rice Bran Powder

2023-06-09 , Kasman N.A.S. , Mohd Sharizan Md Sarip , Mohd Asraf Mohd Zainudin

The effect of drying process of the water extracted rice bran powder on the protein-thiol and physicochemical properties was investigated. The rice bran extracted was prepared with water as solvent at high temperature and pressure using the autoclave equipment (121 °C, 15 psi, and 20 min) before undergone drying method using either spray dryer (BṺCHI Mini Spray Dryer B-290, LABOGENE, Denmark) or freeze dryer (CoolSafe 4-15L Freeze Dryers, LABOGENE, Denmark). The water-extracted rice bran powder (WRBP) yield, protein-free thiol compound, physical and functional properties were analyzed. The protein-thiol concentration was quantified using the 5,5 dithiobis (2-nitrobenzoic acid (DTNB) method while protein concentration was directly measured at 280 nm by UV-Vis Spectrophotometer. This was followed by physical properties (water solubility) and functional properties (foaming capacity and stability, water and oil absorption capacity) analysis. The powder yield of freeze-dried WRBP (7.69 ±0.29%) was significantly (p<0.05) higher than spray-dried WRBP (4.43 ±0.24%). The protein concentration of spray-dried WRBP (4.02 ±0.36 mg/mL) was higher than freeze-dried WRBP (3.91 ±0.75 mg/mL). Meanwhile, freeze-dried WRBP (1.22 ±0.36 nmol thiol/mg protein) had a higher thiol concentration than spray dried WRBP (1.18 ± 0.30 nmol thiol/mg protein). However, both results were not significantly different (p>0.05) between the drying method. Spray-dried WRBP had significantly (p<0.05) higher solubility in water compared to that of freeze-dried WRBP with values 1.22 ± 0.21% and 0.63 ± 0.34%, respectively. Freeze-dried WRBP had higher water and oil absorption capacity. There was no significant difference (p>0.05) in foaming capacity and stability for both drying treatments. In conclusion, the drying method for WRBP production could significantly affect the WRBP physicochemical properties.

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Influence of Nozzle Diameter Towards Velocity Distribution in Spray Dryer Via Computational Fluid Dynamics

2022-01-01 , Mohammad Azrul Rizal Alias , Mohd Sharizan Md Sarip , Mohd Al-Hafiz Mohd Nawi , Wan Azani Wan Mustafa , Yang L.W. , Abdullah Abdul Samat , Raja Ibrahim R.M.Z. , Shabri M.S.M.

Spray drying is commonly used in the chemical and food industries and is the preferred drying process for many materials, such as dairy products, foods, pharmaceuticals, polymers, etc. This research focuses on the effect of the nozzle diameter in the spray drying region against velocity distribution. The results from previous studies have shown that CFD can be a useful tool for predicting the pattern of gas flow and particle histories such as temperature, velocity, time of residence and place of effect. The predictions from these model were validated against reported experimental results, and other simulations. From the simulation analysis, the present studies have identified the performance in the spray dryer through with different size of spray nozzle diameter as the parameter. For the nozzle diameter part, the smaller the spray nozzle diameter, the higher the velocity of the droplets with beneficial to the thermal efficiency of drying particles. The statements finding from the simulation of nine different case study with different nozzle diameter found that the higher velocity has been identified in simulation results due to incomplete vaporize droplets. The main impact of this study is to identify the most optimized condition of the spray dryer chamber after the analysis of the results of simulation data. In conclusion, the design on the chamber has many potential ways to be developed and improved. These findings will benefit the designing of spray dryers.

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Parametric Study on The Rice Bran Protein Extraction Process Using Water as a Solvent

2022-01-01 , Kanapathy M. , Mohd Sharizan Md Sarip , Syahrul Affandi Saidi , Mohd Aminudin Jamlos , Aris N.I.A.

Rice bran is a by-product resulting from the milling process that is frequently underutilized as cattle food or disposed through open-burning despite of its high nutritional and nutraceutical properties. Thus, this research aims in recognizing and exploring rice bran and its extraction methods that could further cultivate in the industry. This study focuses on the rice bran extraction process using water assisted with ultrasonication. The relationship between the operational parameters such as the temperature, extraction time and sample-to-solvent ratio to the protein yield were studied. The rice bran protein was subjected to the surface functional group analysis using Fourier-transform infrared spectroscopy (FTIR). As a conclusion, the extraction temperature of 60°C, sample to solvent ratio of 10 % and extraction time of 25 mins were chosen as the best conditions for the protein extraction. The extraction of the protein from rice bran is highly profitable due to its nutritional and nutraceutical properties as well as it is readily available at low cost.

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Prediction of phase equilibria in ternary diagram for system consist of Hot Compressed Water (HCW) with crude palm oil (CPO) and others minor component using thermodynamic model

2024-05-10 , Mohd Sharizan Md Sarip , Nik Muhammad Azhar Nik Daud , Mohd Asraf Mohd Zainudin , Idham Z. , Adilah Anuar , Mohd Aminudin Jamlos , Amirul Ridzuan Abu Bakar

Knowledge on phase equilibria for the separation process are important. It helps to improve the extraction efficiency through parameter optimization. Nowadays, hot compressed water extraction (HCWE) is getting the interest for the crude palm oil (CPO) extraction. Therefore, the phase equilibria of system consist of Hot compressed water (HCW) and CPO component are crucial. In this study, prediction of phase equilibria in ternary diagram for system consist of HCW with CPO and others minor component was conducted using thermodynamic model. The prediction was conducted using modified UNIFAC. CPO and other minor components namely palmitic acid which represent FFA, β-carotene, α-tocopherol, α-tocotrienol and α-tocotrieno was totally insoluble in HCW. The solubility of HCW in CPO was effect by the temperature and concentration of palmitic acid, β-carotene and α-tocopherol. However, the effect of these minor component concentration was inrrelevant since the concentration of these component was lower in the CPO for less than 1%. The solubility of HCW in CPO need to be monitored to avoid the hydrolysis which can deteriorate the CPO and degrade its quality.

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Quality and stability of rice bran protein powder at different storage condition

2020-12-18 , Mohd Sharizan Md Sarip , Musa N.A. , Syahrul Affandi Saidi , Mansor M.R.

Rice bran contain higher amount of protein, vitamins, essential fatty acids, minerals, dietary fiber and sterol attribute to large food application. The possible application of rice bran protein in food industry are hampered due to the limited information on its stability which may be caused by the rancidity due to the exposure of its oil to lipase. Therefore, stability study for protein extracted form rice bran is essential to evaluate the protein quality at different possible storage condition. Three different storage condition was evaluated; 1. Sample 1 (Store in freezer), 2. Sample 2 (Store in close container in room temperature) and 3. Sample 3 (Store in open container in room temperature). Protein was extracted using hot water process at temperature of 120 C for 20 minutes and dried using spray drying process. All sample are monitored for 5 days for its protein composition, water activity, moisture content and pH. The functional group of the sample where analysed using Fourier transform infrared radiation (FTIR). As a conclusion, the storage condition gives an effect on the water activity and moisture content of the rice bran protein product. However, it not given the significance effect on protein concentration and pH.

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Reduced Graphene Oxide UWB Array Sensor: High Performance for Brain Tumor Imaging and Detection

2023-01-01 , Mohd Aminudin Jamlos , Jamlos M.F. , Wan Azani Wan Mustafa , Othman N.A. , Mohamad Nur Khairul Hafizi Rohani , Syahrul Affandi Saidi , Mohd Sharizan Md Sarip , Mohd Al-Hafiz Mohd Nawi

A low cost, with high performance, reduced graphene oxide (RGO) Ultra-wide Band (UWB) array sensor is presented to be applied with a technique of confocal radar-based microwave imaging to recognize a tumor in a human brain. RGO is used to form its patches on a Taconic substrate. The sensor functioned in a range of 1.2 to 10.8 GHz under UWB frequency. The sensor demonstrates high gain of 5.2 to 14.5 dB, with the small size of 90 mm × 45 mm2, which can be easily integrated into microwave imaging systems and allow the best functionality. Moreover, the novel UWB RGO array sensor is established as a detector with a phantom of the human head. The layers’ structure represents liquid-imitating tissues that consist of skin, fat, skull, and brain. The sensor will scan nine different points to cover the whole one-sided head phantom to obtain equally distributed reflected signals under two different situations, namely the existence and absence of the tumor. In order to accurately detect the tumor by producing sharper and clearer microwave image, the Matrix Laboratory software is used to improve the microwave imaging algorithm (delay and sum) including summing the imaging algorithm and recording the scattering parameters. The existence of a tumor will produce images with an error that is lower than 2 cm.

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Effect of Nozzle Diameter on Temperature Behavior in Spray Dryer Via Computational Fluid Dynamics

2022-01-01 , Mohammad Azrul Rizal Alias , Mohd Sharizan Md Sarip , Wan Azani Wan Mustafa , Mohd Al-Hafiz Mohd Nawi , Yang L.W. , Samat A.A. , Raja Ibrahim R.M.Z. , Shabri M.S.M.

Spray dryer comes at the end of the processing line since it is a critical step in monitoring the quality of the final product. It has certain advantages such as rapid drying rates, a wide range of operating temperatures and short residence times. This research focuses on the effect of inlet air temperature in the spray dryer chamber. The result from previous studies has shown that the increment of the inlet air temperature has caused the dying rate to increase. From the simulation, we have identified the performance in the spray dryer with different inlet air temperature as the parameter. The higher the inlet air temperature, the less the number of incomplete particles inside the spray dryer chamber, the more the success of the drying particles. The most optimized condition of inlet air temperature inside the spray dryer chamber from different parameters was identified as 225 K after the analysis on the results of simulation data. The main impact of this study is to increase the efficiency of drying particles inside the spray dryer chamber via computational fluid dynamics. In conclusion, researchers should concentrate more on the feed inlet air temperature of spray drying for future analysis because it plays an important role that can influence the impact on droplet conversion.

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A Three-Level Factorial Model for Maximising Protein Extraction from Rice Bran with Choline Chloride: Glycerol

2024-05-01 , Ramlee K.A.F.K. , Daud N.M.A.N. , Mohd Sharizan Md Sarip , Amirul Ridzuan Abu Bakar , Zainudin M.A.M.

Rice bran, a by-product of rice milling, contains four protein types – albumin, globulin, prolamin, and glutelin – known for their hypoallergenic nature and nutritional advantages. To enhance rice bran's worth, its protein was isolated using deep eutectic solvent (DES). The influence of three operational factors (temperature, extraction time, and rice bran to DES ratio) on protein yield was evaluated post-conversion using three level factorial design. Optimal conditions at 80°C, 3 hours, and 1:5 rice bran to DES ratio yielded the highest extracted rice bran protein (RBP) at 17.49%. Temperature and solvent-to-sample ratio show the lowest p-value, indicating a significant effect on the RBP yield. The validation test shows an error of less than 5% between the experimental and the predicted value, showing the model can be used to predict the RBP yield. The RBP was then characterised using Fourier Transform Infrared Spectroscopy (FTIR), Bradford Assays and SDS-PAGE electrophoresis to confirm the protein presence in rice bran powder. FTIR analysis showed the existence of amide I, amide II, aliphatic groups and amine or hydroxyl groups in the RBP. Furthermore, Bradford assays analysis indicated 23.89 ± 0.75% protein content in rice bran powder. SDS-PAGE electrophoresis shows the existence of Albumin and globulin in the RBP. In conclusion, a full three level factorial can be used to model and predict the optimal condition for protein extraction from rice bran using ChCl-Gly DES.

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