Farizul Hafiz Kasim
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Farizul Hafiz Kasim
Official Name
Farizul Hafiz, Kasim
Alternative Name
Hafiz Kasim, Farizul Hafiz
Hafiz Kasim, Farizul
Kasim, Farizul Hafiz
Main Affiliation
Scopus Author ID
36925239100
Researcher ID
GBO-1567-2022

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Turning oil palm empty fruit bunch waste into substrate for optimal lipase secretion on solid state fermentation by Trichoderma strains

2017-12-01 , Haliru Musa , Pang Chien Han , Farizul Hafiz Kasim , Subash Chandra Bose Gopinath , Mohd Azmier Ahmad

This study is aimed to improving extracellular lipase production by Trichoderma strains using oil palm empty fruit bunch (OPEFB) medium on solid state fermentation with the assistance of statistical optimization. Two selected Trichoderma strains (Trichoderma sp.1 and Hypocrea neorufa.1) with high lipase producing potential were employed in a stepwise optimization. Initially, the influence of nutritional (carbon, nitrogen sources, and inducers) and environmental (moisture and temperature) factors on lipase production was evaluated using One-Factor-At-A-time (OFAT) method. Next, three most influential factors (Glucose, Urea and Olive oil) resulting from OFAT were optimized for lipase production by Trichoderma sp.1 using the central composite design under response surface methodology. Experiments conducted under optimum conditions (10.38% (w/w) glucose, 0.86% (w/w) urea, and 7.38% (v/w) olive oil) were further improved the lipase production from 0.195 to 0.390 Ug−1, which is about 2 folds greater than un-optimized condition. This study demonstrated that OPEFB can be utilized as inexpensive substrate for improving the lipase secretion by Trichoderma sp.1.

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Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs

2019-07-15 , Musa H. , Farizul Hafiz Kasim , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Chinni S.V. , Ahmad M.A.

A report on the de novo Whole Genome Sequence (WGS)of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA)genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.

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