Mohamad Fahrurrazi Tompang
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Preferred name
Mohamad Fahrurrazi Tompang
Official Name
Mohamad Fahrurrazi, Tompang
Alternative Name
Fahrurrazi Tompang, Mohamad Fahrurrazi
Tompang, Mohamad Fahrurrazi
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Scopus Author ID
56527099200
Researcher ID
GGZ-1964-2022

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Isolation and characterization of polyhydroxyalkanoate producing microorganisms from molasses wastewater

2023 , Natasha Yasmin Hasnol Azahari , Naresh Sandrasekaran , Deiyban Rau , Mohamad Fahrurrazi Tompang , Kunasundari Balakrishnan

Polyhydroxyalkanoates (PHAs) are polyesters generated by numerous microorganism, where it served as a source of energy and carbon store for bacteria. These plastics are biodegradable, eco-friendly and recyclable. In the present study, 2 strains were successfully isolated and identified from molasses wastewater using staining with Nile Red A. Genotypic identification revealed that the isolates belong to species of Pseudomonas alcaliphila and Pseudomonas hydrolytica. Growth curves of both strains were constructed using POLYMATH software showed similar growth patterns and rates. The PHA accumulation of Pseudomonas alcaliphila and Pseudomonas hydrolytica were recorded at 55% and 45% of dry cell weight. The PHA functional group was further determined through Fourier Transfer InfraRed Spectroscopy (FTIR) analysis.

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Isolation and characterization of laccase secreting microorganism from Gua Kelam 2, Perlis

2023 , Natasha Yasmin Hasnol Azahari , Nor Anis Syafiqah Suhaily , Sandrasekaran Naresh , Mohamad Fahrurrazi Tompang , Kunasundari Balakrishnan

The current study, involves isolation and characterization of laccase producing microbes from Gua Kelam 2, Perlis, Malaysia. In our study, all isolates were cultivated on nutrient agar containing guaiacol. Only one isolate (GK1) was detected as a positive laccase producer which was then subjected for growth curve and kinetic analysis using nutrient broth and minimal salt medium. For nutrient broth, short exponential phase was recorded from 2nd h until 4th h in contrast to minimal salt medium that exhibited exponential phase from 8th h until 24th h. The highest laccase activity was recorded in nutrient broth (4.50 U L-1) at 4th h incubation compared to minimal salt medium (3.72 U L-1) at 30 h. The isolate GK1, was characterized to be rod shape and Gram positive bacterium. Based on the 16S rRNA sequencing, the isolate determined to be Bacillus subtilis.

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