Amira Farzana Samat
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Preferred name
Amira Farzana Samat
Official Name
Amira Farzana, Samat
Alternative Name
Samat, Amira Farzana
Samat, Amira F.
Samat, A. F.
Farzana, S. Amira
Main Affiliation
Scopus Author ID
57200990567
Researcher ID
DQS-3846-2022

Research Output
Now showing 1 - 3 of 3
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Optimization of an ultrasound-assisted extraction method for phenolic content in Momordica Charantia seeds and its antifungal activity against Pleurotus Ostreatus Green Mold Pathogen

2023-09 , Norhidayah Abd Aziz , Noor Hasyierah Mohd Salleh , Nur Umi Masjida Ahmad Fauzi , Azlina Harun Kamaruddin , Muhamad Ikmal Sirozi , Zarina Zakaria , Subash Chandra Bose Gopinath , Amira Farzana Samat

Phenolic compounds from fruits and plant parts showed several biological properties such as antifungal activity. This study reports the optimized conditions for total phenolic content (TPC) extraction from Momordica charantia seeds using ultrasound-assisted extraction and its antifungal activity against Pleurotus pulmonarius green mold (GM) pathogens. Using water as solvent, a response surface methodology (RSM) was employed involving three independent factors including liquid to solid ratio (LSR) (10:1–60:1), pH (3.0–9.0), and extraction time (2–8 min). ANOVA suggested only parameters of pH and LSR were significant to produce TPC yield of 166.7 mg GAE/g, at optimized conditions of pH9.0 and LSR 10:1. The antifungal activity of optimized momordica seed extract (MSE) was tested against P. pulmonarius GM pathogens; Trichoderma sp., Aspergillus flavus and Penicillium citrinum. MSE presented antifungal activities against all tested strains, in a dose and strain-dependent manner. The highest growth inhibition was achieved when using 2.5 ml of extract for both Trichoderma sp. and P. citrinum with 63.1% and 89.2% inhibition, respectively. Meanwhile, 3.5 ml of extract was needed to achieve 79% inhibition on A. flavus. Conclusively, the use of non-toxic green solvent such as water, assisted with ultrasound power, is useful to produce MSE with antifungal activities as an alternative approach to chemical fungicides for the protection of grey oyster mushroom from GM pathogen.

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Biodeterioration of pre-treated polypropylene by Aspergillus terreus and Engyodontium album

2023 , Amira Farzana Samat , Dee Carter , Ali Abbas

Polypropylene (PP) has raised severe environmental issues concerning its non-degradability, with a current recycling rate of only 1%. This current study utilises Aspergillus terreus ATCC 20542 and Engyodontium album BRIP 61534a to break down PP while focusing on pre-treatment. Polypropylene granule (GPP), film (FPP) and metallised film (MFPP) are pre-treated by either UV, heat, or Fenton’s reagent. UV and heat-treated MFPP by A. terreus exhibits notable weight loss percentage (25.29% and 22.13%, respectively). Biomass production, reduction rate, Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) analyses further validate the degradation rate. A. terreus incubated with UV-treated MFPP produced a relatively high biomass yield of 1.07 mg/ml. Reduction in carbonyl index and surface morphological changes reveal consistent biodeterioration evidence. This investigation demonstrates that A. terreus and E. album can grow on, change, and utilise PP as a carbon source with pre-treatments’ aid, promoting the biological pathways for plastic waste treatment. © 2023, The Author(s).

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Growth Optimisation of Bacillus subtilis in medium supplemented with prebiotic gum Arabic

2020-12-18 , Norhidayah Abd Aziz , Noor Hasyierah Mohd Salleh , Abu Hassan A.F. , Amira Farzana Samat , Adilah Anuar , Siti Aminah Mohd Hassan , Lian Ou Li

Bacillus subtilis is a type of gastrointestinal bacteria that build up a healthy gut microbiota. The bacterial species has been well documented to involve in maintaining a healthy gut homeostasis in which disruption in it's microbial composition or "dysbiosis"has recently been linked to obesity. Probiotic in vivo restoration of dysbiosed microbiota have been proved successful in obese CD-1 mice model. However, prebiotic study using Gum Arabic (GA) assessing to optimise an in vitro lumen system specifically targeting to enhance the growth of B. subtilis are still lacking since this bacteria are depleted in obese individuals. Thus, this study aimed to establish the optimal growth conditions in simulated in vitro lumen system and to the best of our knowledge, this is the first in vitro study attempted to optimize the growth of B. subtilis in medium supplemented with prebiotic GA. Growth screening analysis suggested an optimal dosage of 1.0% and 0.5% glucose and GA, respectively. The highest growth rate was recorded at 0.7995 hours-1 with doubling time of 52.02 minutes with extended period of stationary phase. The optimal GA concentration and fermentation conditions were determined at 0.67%, pH 7.4 and temperature of 37 C. The validated suggested model indicates that the supplementation of GA into an optimal fermentation systems is promising to enhance the growth of gut microflora Bacillus subtilis, for restoration of a dysbiosed gut microbiota in vitro.

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