Ahmad Mukhlis Abdul Rahman
Thumbnail Image
Preferred name
Ahmad Mukhlis Abdul Rahman
Official Name
Ahmad Mukhlis, Abdul Rahman
Alternative Name
Abdul Rahman, Ahmad Mukhlis
Main Affiliation
Researcher ID
HGA-7390-2022

Research Output

Filters

3
Reset filters

Settings
Now showing 1 - 3 of 3
  • Publication
    Morphological variability identification of Harumanis Mango (Mangifera indica L.) harvested from different location and tree age
    (Penerbit Universiti Sains Malaysia, 2020-08)
    Siti Nur Arina Yusuf
    ;
    Ahmad Mukhlis Abdul Rahman
    ;
    Zarina Zakaria
    ;
    Vijay Kumar Subbiah
    ;
    Maz Jamilah Masnan
    ;
    Zakaria Wahab
    Harumanis is one of the main signatures of Perlis with regards to its delightful taste, pleasant aroma and expensive price. Harumanis authenticity and productivity had become the remarks among the farmers, entrepreneurs, consumers and plant breeders due to the existence of morphological characteristics variation among the fruits and high production cost. Assessment of Harumanis morphological characteristics of natural population and different tree ages may represent a possible source of important characteristics for development and breeding purposes of Harumanis. The aim of this study is to evaluate the morphological variation of Harumanis collected from different location in Perlis and tree age. A total of 150 Harumanis fruits from 50 trees with three different stages of development (young, middle-aged and old) were characterised using 11 traits; 10 quantitative and one qualitative morphological trait. The ANOVA analyses in combination with Dunn’s pairwise and Kruskal-Wallis multiple comparison test able to point out the existence of environmental factor and age influence towards the significant different of identified morphological traits except for Total Soluble Solid (TSS) and pulp percentage. Five clusters of 50 Harumanis accessions reflect a grouping pattern which not according to neither geographical region nor age. The result of Principal Component Analysis (PCA) using the first two principal components (PCs) provided a good approximation of the data explaining 84.09% of the total variance which majorly contributed by parameters of weight, fruit dimensional characteristics, peel percentage and hue angle, h. Preliminary screening of important morphological characteristics which contribute to the phenotypic diversity of Harumanis is successfully achieved. The findings can be employed by the plant breeders and farmers for the establishment of standard grading of Harumanis and advancement of breeding crop of Harumanis in future.
      1  7
  • Publication
    Chloroplast DNA sequence of trnR-N and trnL-F regions in Harumanis mango from different orchards in Perlis, Malaysia
    (IOP Publishing Ltd, 2020)
    Ahmad Mukhlis Abdul Rahman
    ;
    SFM Sabri
    ;
    Yusuf, Arbai
    ;
    Zarina Zakaria
    ;
    SV Kumar
    Harumanis is a premium mango cultivar widely known for its sweet taste, aroma and vibrant flesh colour. To date, the genetic identification of this mango based on multiple conserved DNA region using samples from different orchards has never been reported. The aim of this research is to identify the genetic signature of Harumanis mango at molecular level by analyzing chloroplast DNA sequences of the trnL-F and trnR-N regions. DNA samples were extracted from a total of 15 Harumanis samples collected from five selected orchards using Cetyl Trimethyl Ammonium Bromide (CTAB) extraction procedure. The extracted DNA and the PCR-amplified products were analyzed through gel electrophoresis and were then subjected to DNA Sequencing and in silico analysis. The obtained sequences were compared with the sequences available in the GeneBank. BLAST search for both the trnR-N and trnL-F regions confirmed that all the 15 samples belong to Mangifera indica with a 99% sequence identity. In addition, the trnL-F sequences were 99% identical to a number of specific mango cultivars such as, Tommy and Arunika. However, the trnR-N sequences were less informative as it gave hits to only two mango accessions (e.g. Mangifera indica voucher PDBK 2014-0249). It is postulated that the plastid trnR-N may be a potential candidate region for the development of the Harumanis genetic signature. The results may be used to complement other molecular data for the development of a genetic barcode for Harumanis.
      1  22
  • Publication
    Isolation of cellulolytic microorganism from food waste-fertilized soil
    (AIP Publishing Ltd., 2023)
    Aliaa Rasyidina Idrus
    ;
    Nor Hidayah Abd Aziz
    ;
    Zarina Zakaria
    ;
    Ahmad Mukhlis Abdul Rahman
    Uncontrolled disposal of waste from cellulosic materials can add burden to the existing environmental crises. The aim of the study is to isolate microorganisms from food waste-fertilized soil and identification of the microorganism species by genotyping. Soil samples enriched with fruits, rice and vegetables wastes were collected, diluted and spread on agar supplemented with carboxymethylcellulose (CMC) for induction of growth and cellulase production. Following screening of cellulolytic activity, isolates with significant cellulolytic index (CI) were chosen for molecular identification by PCR, using the 16S rRNA as target sequence in combination with in silico analysis. Four out of eleven microorganisms isolated from fruits and vegetable-rich soils produced a CI ranging from 0.43 to 2.00. Of these, only two isolates produced clear bands on PCR with approximately 1500 bp in size. In silico analysis of the isolates suggested species of Ochrobactrum sp. and Bacillus sp. These microorganisms have the potential to be used for decomposition of the cellulosic wastes.
      1  20