Ahmad Mukhlis Abdul Rahman
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Preferred name
Ahmad Mukhlis Abdul Rahman
Official Name
Ahmad Mukhlis, Abdul Rahman
Alternative Name
Abdul Rahman, Ahmad Mukhlis
Main Affiliation
Researcher ID
HGA-7390-2022

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  • Publication
    Differential Display Reverse Transcription Polymerase Chain Reaction (DDRT-PCR) for Grey Oyster mushroom samples grown with acoustic sound treatment
    (IOP Publishing, 2023)
    Tham Khai Song
    ;
    Norsahira Ezrin Mohd Zamri
    ;
    Roshita Ibrahim
    ;
    Johan Ariff Mohtar
    ;
    Hartinee Abbas
    ;
    Ahmad Mukhlis Abdul Rahman
    Grey oyster mushroom is the most often farmed and marketed type of mushroom for household consumption in Malaysia. The mushroom cultivation takes a long time due to its complicated mycelium growth. Several treatments were introduced such as acoustic sound treatment to increase the growth rate and quality of the crop, but there is no in-depth study regarding the genetic expression of the genes affected. This study aims to evaluate the Differential Display Reverse Transcriptase-Polymerase Chain Reaction (DDRT-PCR) for grey oyster mushroom samples grown with acoustic sound treatment. The mushroom was subjected to the treatment and the RNA was extracted from the mushroom samples and converted into cDNA before undergoing DDRT-PCR. Ten Differential Expressed Transcripts (DETs) that were successfully identified based on the differences between the intensity and absence of amplicons were sent for gene sequencing and BLAST through the NCBI database to obtain relevant results regarding the possible gene annotation. Seven out of ten DETs hit potential genes encoding for housekeeping and structural and development functions. The results showed that acoustic sound treatment did affect the expression of certain genes differently as captured by DDRT-PCR analyses and offers new ideas for the development of ecological agriculture.
  • Publication
    DNA barcoding of common Malaysia spiders using the Cytochrome Oxidase I (COI) gene
    (The Malaysian Society of Applied Biology, 2020)
    Ahmad Mukhlis Abdul Rahman
    ;
    Kek Chian Koay
    ;
    Johan Ariff Mohtar
    ;
    Anwardi Jamil
    ;
    Mohd Mushahril Abdul Shukor
    ;
    Nurul Ain Harmiza Abdullah
    For the last twenty years, many newly described spiders were collected from Malaysia and in fact, more than 11,000 species were recorded in Peninsular Malaysia as well as in Sabah and Sarawak states. Scientists have put an immense effort on untangling the spider biology from its physical structure and behavior to silks and venoms. However, working with spiders is impeded by the difficulties in species identification via solely morphological methods. Thus, DNA barcoding is an alternative technique that employs standard fragment to facilitate species identification. Isolation of genomic DNA from three Malaysian spiders were performed using NucleoSpin® DNA insect extraction kit. Amplification of reference mitochondrial cytochrome oxidase I (COI) gene employing PCR with two set of primers followed by the DNA sequencing and validation through phylogenetic analysis were carried out. The commercial extraction kit was effective for the recovery of good quality of intact genomic DNA band as indicated by the integrity analysis. Both set of primers successfully amplified 100% of the samples with approximately 600 – 700 bp of PCR products. The obtained sequences (610 bp to 692 bp) were compared with the sequences available in Gene Bank. BLAST and phylogenetic analysis revealed that the analyzed individual samples belong to Nephila pilipes, Neoscona nautica and Crossopriza lyoni, respectively. Phylogenetic analysis provided unique insight into the evolutionary relationship of each analyzed sample. This study aids in an accurate identification of the selected local spider species at molecular level using the COI gene.
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