Ahmad Anas Nagoor Gunny
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Preferred name
Ahmad Anas Nagoor Gunny
Official Name
Ahmad Anas, Nagoor Gunny
Alternative Name
Nagoor Gunny, Ahmad Anas
Gunny, A. A.N.
Nagoor Gunny, A. A.
Ahmad Anas, N. G.
Gunny, Ahmad Anas Nagoor
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Scopus Author ID
55940833700
Researcher ID
AFF-6251-2022

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Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs

2019-07-15 , Musa H. , Farizul Hafiz Kasim , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Chinni S.V. , Ahmad M.A.

A report on the de novo Whole Genome Sequence (WGS)of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA)genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.

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Harnessing oregano nanoemulsion: a novel solution to combat curvalaria-induced fruit rot and preserve mango quality

2025-02 , M. J. Gidado , Ahmad Anas Nagoor Gunny , Nor Amirah Azwa Norizal , Subash Chandra Bose Gopinath , Chalermchai Wongs-Aree , Muaz Mohd Zaini Makhtar , Raja Hasnida Raja Hashim , Mohd Hishamuddin Che Mat

Mango is a significant global fruit crop, producing over 1,000 million tonnes annually. However, postharvest losses due to pathogenic fungal infections are considerable, exacerbated by the continuous use of synthetic fungicides, which pose risks of fungal resistance and environmental harm. This study assessed the effectiveness of Origanum vulgare-based nanoemulsion against mango postharvest diseases and quality preservation. Results indicate that the O. vulgare nanoemulsion (Ore-S1-15) exhibited optimal properties, including small droplet size, low polydispersity, and stable pH. FTIR analysis identified key functional groups, while GC-MS results revealed prominent components with isopropyl myristate being the major constituent at 42.41%, followed by isopropyl palmitate (25.53%), oleic acid (4.57%), diethyl phthalate (3.84%), estagole (2.09%), 2-(phenylmethylene)-octanal (1.17%), cyclopentane acetic acid (0.85%), benzoic acid (0.34%), and coumarin (0.26%) as minor constituents. In vitro test of the Ore-S1-15 nanoemulsion against Curvularia sp. demonstrated significant antifungal activity, with 79.51 ± 0.95% conidia inhibition. Additionally, in vivo test showed a reduction in disease incidence on wounded mangoes. The Ore-S1-15 nanoemulsion enhanced quality parameters by delaying colour changes, reducing weight loss and steadily maintaining the total soluble solids. Thus, Ore-S1-15 nanoemulsion emerges as a promising and eco-friendly alternative to synthetic fungicides for controlling mango postharvest diseases and increasing shelf life while preserving quality.

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Challenges of postharvest water loss in fruits: Mechanisms, influencing factors, and effective control strategies – A comprehensive review

2024-09-01 , Gidado M.J. , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Ali A. , Wongs-Aree C. , Noor Hasyierah Mohd Salleh

Water loss is a critical concern in postharvest fruit technology, significantly impacting fruit quality, shelf life, and market value. This phenomenon involves the loss of moisture from fruit tissues, leading to physiological changes, reduced quality, and lower market value, which can contribute to food waste. Consumers perceive fruit with visible signs of water loss as lower quality. Therefore, minimizing water loss is essential through proper postharvest handling and storage practices, including temperature and humidity control, cooling, humidification, and the application of edible coatings and new modified coating techniques. Despite the importance of managing water loss, comprehensive information on its mechanisms and contributing factors in postharvest fruit technology is scarce. This paper aims to provide insights into the mechanisms, impact, influencing factors, and control strategies related to water loss in the context of postharvest fruit technology.

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Cellulose nanocrystals from bleached rice straw pulp: acidic deep eutectic solvent versus sulphuric acid hydrolyses

2021-07-01 , Lim W.L. , Ahmad Anas Nagoor Gunny , Kasim F.H. , Subash Chandra Bose Gopinath , Nor Helya Iman Kamaludin , Arbain D.

The present work aims to investigate the feasibility of oxalic acid-choline chloride deep eutectic solvent (OA-ChCl DES), which serves as a promising green solvent that utilized in the acidic deep eutectic solvent (DES) hydrolysis. Oxalic acid-choline chloride DES cellulose nanocrystal (OA-ChCl DES CNC) was isolated from the bleached DES treated pulp (BP) through the acidic DES hydrolysis using 1:1 molar ratio of OA-ChCl DES. The functional groups, crystallinity index, morphological structure, particle size, zeta potential, thermal stability and surface chemistry of the OA-ChCl DES CNC were compared with the sulphuric acid cellulose nanocrystal (SA-CNC) that prepared via sulphuric acid hydrolysis. The findings revealed the presence of negatively charged carboxyl groups on OA-ChCl DES CNC surface after the acidic DES hydrolysis. The physicochemical analyses verified that the OA-ChCl DES CNC was in nano-sized range with polydispersity index (PdI) of 0.56, indicating slightly monodispersed nanoparticles. A stable OA-ChCl DES CNC colloidal suspension with zeta potential value of −52.1 ± 5.2 mV was obtained. The OA-ChCl DES CNC outweighed the SA-CNC in term of thermal stability (288 °C) despite having a slightly lower crystallinity index (76.7%). In fact, the OA-ChCl DES CNC with a yield of 55.1% was achieved through the acidic DES hydrolysis, suggesting that the OA-ChCl DES was capable of promoting efficient cleavage of strong hydrogen bonds in BP. Graphic abstract: [Figure not available: see fulltext.]

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Delaying the Ripening of Banana Fruit and Increased Storage Shelf-Life Using Hydrophobic Deep Eutectic Oil (Menthol–Thymol)-In-Water Nanoemulsion Coating

2024-01-01 , Gidado M.J. , Ahmad Anas Nagoor Gunny , Sri Alamelu Sankari R. , Subash Chandra Bose Gopinath , Wongs-Aree C. , Shukor H. , Ibrahim R.

Hydrophobic deep eutectic oil (menthol-thymol)-in-water nanoemulsion was developed to preserve the quality of banana fruit and extend their storage shelf life. Storage preservation of bananas presents several challenges due to their high respiration rate, ethylene production, and susceptibility to various physiological and biochemical changes. The storage shelf life of bananas is relatively short compared to other fruits due to the combination of rapid ripening and sensitivity to ethylene and temperature. In this study, the effectiveness of HyDEN treatment was assessed on changes in banana fruit’s physiological parameters such as firmness, weight loss, total soluble solids, titratable acidity, and colour for 14 days storage time [25 °C ± 1 °C, 67% relative humidity (RH)]. HyDEN treatment preserved the physicochemical characteristics of banana fruit and was effective in delaying the ripening process. The storage shelf life of banana fruit treated with HyDEN increased for 14 days compared to control that ripened and physically damaged after 8 days of storage. This study provided a new delivery system for applying HyDEN as an edible coating to fruit after harvest preservation.

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Microwave-assisted solvent-free extraction of essential oil from Coleus aromaticus: anti-phytopathogenic potential for fruit post-harvesting

2021-04-01 , Ahmad Anas Nagoor Gunny , Misnan N.M. , Subash Chandra Bose Gopinath , Salleh N.H.M. , Hashim R.H.R. , Mat M.H.C.

This work evaluates the fungicidal effect of essential oil from Coleus aromaticus (C. aromaticus) by solvent-free microwave-assisted extraction with a yield of 0.54%. Fourier-transform infrared spectroscopy was utilised to identify the functional groups, which were O–H, C–O, C–H, and C=C. Gas chromatography–mass spectrometry analysis was performed to determine the primary essential oil components, namely, thymol (92.62%), thymoquinone (2.64%), creosol (1.77%), linalool (1.68%), p-Cymene-2,5-diol (0.73%), and p-Cymene (0.56%). The inhibitory effect of essential oil extracted from C. aromaticus against the isolated fungi, Aspergillus niger from mango, was investigated. The mycelial growth inhibition of the extracted essential oil by the poisoned food test and disc diffusion assay showed the reduction at 79.63 ± 1.7 and 70.45 ± 6.54%, respectively. In vivo experiment was conducted with artificially wounded and unwounded mangoes, applying the extracted essential oil to the wounded mangoes inoculated with A. niger that could decrease the disease incidence from 100 to 58.33 ± 14.43%. Meanwhile, the treatment of the extracted essential oil did not affect the quality of the mango and it also shows improvement in weight loss reduction of the mango in comparison to the chemical fungicide and untreated mango. Hence, essential oil from C. aromaticus by solvent-free microwave-assisted extraction could be used as an effective control for the fruit spoilage and potential source of fruit preservative.

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THE EFFECT OF NANOFILLERS ON THE FUNCTIONAL PROPERTIES OF PLA AND CHITOSAN BASED FILM

2023-01-01 , Hashim R.H.R. , Ahmad Anas Nagoor Gunny , Sam Sung Ting , Kamaludim N.H.I. , Subash Chandra Bose Gopinath

The aim of this study was to develop poly (lactic acid) and chitosan-based films and to examine the effect of cellulose nanocrystal (CNC) as nanofillers on the properties of the films. The biofilms were prepared by solvent casting method. The physical and mechanical properties of the resulting films were examined. SEM analysis showed that the surface of PLA/Cs films smoother when 1% and 2 % of CNC were added. The results for percent of water absorption of the films increased with increasing amount of CNC in PLA/Chitosan matrix. Tensile test results indicated higher TS value by incorporation of 2% of CNC. However, the PLA/Chitosan-CNC films at 3% and 4% CNC concentration exhibited a decreased TS value. PLA/Chitosan films were improved with the addition of a small amount of CNC resulting in PLA nanocomposite, which will be further evaluated for fruit packaging applications. The data obtained through this research could contribute to the establishment of a biofilms with improved the physical and mechanical properties promising significant advantages in term of longer storage life, maintaining safety, and keeping quality of a product especially in fruit packaging.

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Investigating the impact of hydrophobic deep eutectic oil-in-water nanoemulsion on cell membrane degradation and inhibition of C.gloeosporioides in postharvest technology

2025 , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Noor Hasyierah Mohd Salleh , Sunil Salleh , Kunasundari Pareek

This study examines the effects of a hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) on the cell membrane degradation and inhibition of Colletotrichum gloeosporioides. By analyzing post-treatment cell membrane morphology, we gain insights into the efficacy and mechanisms of action of antifungal agents. Significant changes such as disruption, collapse, wrinkling, and lysis were observed in the external morphology of C. gloeosporioides treated with HyDEN. When comparing control and treatment groups, HyDEN demonstrated more pronounced disruption and greater mycelial growth inhibition than Globus 5.5. HyDEN also effectively inhibited spore germination compared to Globus 5.5. Tests on intracellular ion leakage showed that HyDEN caused higher conductivity, indicating significant membrane disruption. Additionally, HyDEN led to a greater release of soluble sugars due to membrane damage compared to Globus 5.5. These findings suggest that HyDEN is a promising antifungal strategy, effectively disrupting cell wall and membrane functionality, and inhibiting fungal growth.

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Cellulose nanocrystal production from bleached rice straw pulp by combined alkaline and acidic deep eutectic solvents treatment: optimization by response surface methodology

2022-07-01 , Lim W.L. , Ahmad Anas Nagoor Gunny , Farizul Hafiz Kasim , Subash Chandra Bose Gopinath

The present study aims to develop a statistical model utilizing the response surface methodology (RSM) to investigate the performance of oxalic acid-choline chloride deep eutectic solvent (OA-ChCl DES) in hydrolyzing amorphous domain of the bleached rice straw pulp (BP), while liberating the oxalic acid-choline chloride DES cellulose nanocrystal (OA-ChCl DES CNC). The process parameters, including temperature, reaction time, and BP to OA-ChCl DES mass ratio, were optimized through the RSM coupled with the face-centered central composite design (FCCCD). The mathematical models were generated, while the analysis of variance (ANOVA) was conducted to determine the most significant factors influencing the response which was measured in terms of yield. The findings revealed that the OA-ChCl DES CNC yield as high as 55.08% was attained under the acidic DES hydrolysis conditions of 79.5 °C, 4 h, and 1:12.64 mass ratio. This regression model enables researchers to predict the OA-ChCl DES CNC yield with respect to the influencing parameters as well as providing an insight for further scale-up process.

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Fabrication of active food packaging based on PLA/Chitosan/CNC-containing Coleus aromaticus essential oil: application to Harumanis mango

2023-12-01 , Hasnida Raja Hashim R. , Ahmad Anas Nagoor Gunny , Sam Sung Ting , Subash Chandra Bose Gopinath , Fong Y.Y. , Pareek S. , Makhtar M.M.Z. , Hafiza Shukor

This study aimed to determine the effect of Coleus aromaticus essential oil (CAEO) on the properties of an active film based on polylactic acid (PLA), chitosan (Cs), and cellulose nanocrystal (CNC) in fruit packaging. The films were fabricated via solvent casting technique. The obtained films’ antimicrobial, antioxidant, microstructural, and mechanical properties were studied. Mechanical properties state how adding essentials oil into film improves the elongation breaks significantly (p < 0.05) by 5.3 and 6.1%, respectively, with the addition of 0.4 wt% and 0.8 wt% CAEO which reflets its flexibility. The antioxidant activity of biopolymer film increased significantly (p < 0.05), with antioxidant values ranging from 6.50 to 57.50% with the elevation of CAEO. The inhibitory impact of the film against pathogenic fungus was evaluated in vitro and in vivo by comparing the film with different concentrations of essential oil (EO), as well as the control and chemical fungicide. Disc diffusion was utilised to test mycelial growth suppression, and the film containing 1.2 wt% EO produced the best results. The biofilm containing 1.2 wt% EO successfully reduced illness incidence in vivo with damaged mango. Scanning Electron Microscopy and Transmission Electron Microscopy imaging were used to observe the incorporation of CAEO in the matrix of the film. All Fourier-Transform Infrared spectra of PLA/Cs/CNC and EO blends exhibited the characteristic bands of PLA-based materials. The results indicate that the PLA/Cs/CNC/CAEO films provide a new way to improve microbial safety and extend the shelf life of mango fruit and have the potential for replacement of petroleum-based plastic for fruit packaging applications at the industrial level.

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