Ahmad Anas Nagoor Gunny
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Preferred name
Ahmad Anas Nagoor Gunny
Official Name
Ahmad Anas, Nagoor Gunny
Alternative Name
Nagoor Gunny, Ahmad Anas
Gunny, A. A.N.
Nagoor Gunny, A. A.
Ahmad Anas, N. G.
Gunny, Ahmad Anas Nagoor
Main Affiliation
Scopus Author ID
55940833700
Researcher ID
AFF-6251-2022

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Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs

2019-07-15 , Musa H. , Farizul Hafiz Kasim , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Chinni S.V. , Ahmad M.A.

A report on the de novo Whole Genome Sequence (WGS)of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA)genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.

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Delaying the Ripening of Banana Fruit and Increased Storage Shelf-Life Using Hydrophobic Deep Eutectic Oil (Menthol–Thymol)-In-Water Nanoemulsion Coating

2024-01-01 , Gidado M.J. , Ahmad Anas Nagoor Gunny , Sri Alamelu Sankari R. , Subash Chandra Bose Gopinath , Wongs-Aree C. , Shukor H. , Ibrahim R.

Hydrophobic deep eutectic oil (menthol-thymol)-in-water nanoemulsion was developed to preserve the quality of banana fruit and extend their storage shelf life. Storage preservation of bananas presents several challenges due to their high respiration rate, ethylene production, and susceptibility to various physiological and biochemical changes. The storage shelf life of bananas is relatively short compared to other fruits due to the combination of rapid ripening and sensitivity to ethylene and temperature. In this study, the effectiveness of HyDEN treatment was assessed on changes in banana fruit’s physiological parameters such as firmness, weight loss, total soluble solids, titratable acidity, and colour for 14 days storage time [25 °C ± 1 °C, 67% relative humidity (RH)]. HyDEN treatment preserved the physicochemical characteristics of banana fruit and was effective in delaying the ripening process. The storage shelf life of banana fruit treated with HyDEN increased for 14 days compared to control that ripened and physically damaged after 8 days of storage. This study provided a new delivery system for applying HyDEN as an edible coating to fruit after harvest preservation.

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Water reclamation from palm oil mill effluent (POME): Recent technologies, by-product recovery, and challenges

2023-04-01 , Safa Senan Mahmod , Takriff M.S. , AL-Rajabi M.M. , Abdul P.M. , Ahmad Anas Nagoor Gunny , Silvamany H. , Jahim J.M.

Water scarcity will continue to be a global challenge in the coming years as long as sustainable solutions remain far-fetched. It has become necessary to improve the efficiency of water circulation to reduce the net demand in high water consumption sectors such as agriculture. In palm oil industry, the extraction of crude palm oil from oil palm fruit bunch requires a large amount of water, half of which is discharged as palm oil mill effluent (POME) that is considered a high strength wastewater. The current trend of POME research focuses on moving from conventional open-ponding treatment to an integrated zero-discharge system for better effluent management. In this review, the attempts taken by researchers in laboratory and pilot scale were highlighted to achieve an effluent of high-quality water from POME that can be used as drinking water or recycled as boiler water in the palm oil production process. Membrane technology has recently emerged as one of the most promising separation techniques having a broad spectrum of applications including POME treatment. Furthermore, water reclamation technique involving evaporation can split POME solids and recover clean water in a more sustainable and space-saving manner. Additionally, recovery of by-product, such as biofertilizer and biofuels, is necessary to overcome the cost of the treatment. As a whole, in spite of several challenges, water reclamation from POME can be considered economically feasible system from techno-economic analysis perspective and it can feasibly shift the adopted industrial method from a linear take-use-discharge economy approach to a circular economy by keeping water in circulation.

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Investigating the impact of hydrophobic deep eutectic oil-in-water nanoemulsion on cell membrane degradation and inhibition of C.gloeosporioides in postharvest technology

2025 , Ahmad Anas Nagoor Gunny , Subash Chandra Bose Gopinath , Noor Hasyierah Mohd Salleh , Sunil Salleh , Kunasundari Pareek

This study examines the effects of a hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) on the cell membrane degradation and inhibition of Colletotrichum gloeosporioides. By analyzing post-treatment cell membrane morphology, we gain insights into the efficacy and mechanisms of action of antifungal agents. Significant changes such as disruption, collapse, wrinkling, and lysis were observed in the external morphology of C. gloeosporioides treated with HyDEN. When comparing control and treatment groups, HyDEN demonstrated more pronounced disruption and greater mycelial growth inhibition than Globus 5.5. HyDEN also effectively inhibited spore germination compared to Globus 5.5. Tests on intracellular ion leakage showed that HyDEN caused higher conductivity, indicating significant membrane disruption. Additionally, HyDEN led to a greater release of soluble sugars due to membrane damage compared to Globus 5.5. These findings suggest that HyDEN is a promising antifungal strategy, effectively disrupting cell wall and membrane functionality, and inhibiting fungal growth.

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Inhibition of Aspergillus flavus by Momordica charantia Ethanolic Leaves Extract for Mushroom Protection

2023-06-09 , Noor Hasyierah Mohd Salleh , Zulkarnay Zakaria , Aziz N.H. , Ahmad Anas Nagoor Gunny , Alina Rahayu Mohamed , Munusamy T.

Pleurotus ostreatus, better known as oyster mushroom is one of the world’s widely cultivated mushroom. Infection from pathogenic fungi is one of the major reasons causing losses of mushroom during cultivation. The objective of this study is to investigate the antifungal property of Momordica charantia leaves extract against Aspergillus flavus for mushroom protection. The inhibitory effect of M. charantia leaves extract against A. flavus isolated from infected P. ostreatus mushroom bag was investigated using pour plate technique. Inhibition of A. flavus mycelial growth was the highest at extract concentration of 9.64 v/v %, temperature 26℃ and incubation time of 96 hours with inhibitory value of 70.58 ± 0.00 %, 78.4 ± 0.00 % and 63.33 ± 2.89 % respectively. Structural morphology of the mycelia grown on the treated plate also showed morphological alteration compared to the control plate. Hence, Momordica charantia leaves extract may serves as a promising fungicides alternative to chemical use for controlling invasion of A. flavus on oyster mushroom.

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Anaerobic co-digestion of chicken manure with energy crop residues for biogas production

2021-05-24 , Dawi Cahyono N.A. , Shamsuddin M.R. , Ayoub M. , Mansor N. , Isa N.H.M. , Ahmad Anas Nagoor Gunny

Biogas or bio-methane is an alternative energy source produced by anaerobic digestion (AD) technology whereby the organic matter is degraded by naturally present microorganisms and converted into a combustible gas. This paper focus on the utilization of anaerobic co-digestion (AcoD) method since biogas yield so far remains minimum based on mono- digestion alone due to low energy content. In this work, empty fruit bunch (EFB), oil palm frond (OPF) and sugarcane bagasse (SB) are being used as co-digestion additive since energy crop residues are known for its high carbon and energy content to enhance the process. A series of five 1.5L reactors were set up for a mesophilic condition at temperature 40°C, with initial pH range of 7-8, for 60 days of retention time. The mono-digestion reactor of chicken manure is labelled as DC1, and the co-digestion reactors with sugarcane bagasse, OPF and EFB are labelled as DSB, DOPF and DEFB, respectively. The recorded C/N ratio of co-digestion reactors were improved to be in a favourable range of 15 to 35 with DSB being the highest at 17.74 compared to that of control reactor at 11.35. As a result, total biogas production for DSB increases up to 36% due to its significantly high carbon content from sugarcane bagasse that provide stability for the bacterial growth and its activities. Sugarcane bagasse act as a good carbon source for the AD system, thus enhance the methane production. On the other hand, DOPF and DEFB give 13% and 41% lesser total biogas volume, however, produces much higher methane concentration compared to the control reactor. This shows that DOPF and DEFB give a higher methane yield which defines the more stability and efficiency of digestion process.

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22 Regulations for food packaging materials

2023 , Nur Amalina Amirullah , Mohd Hafif Samsudin , Mohd Nor Faiz Norrrahim , Rushdan Ahmad Ilyas , Norizan Mohd Nurazzi , Mohd Azwan Jenol , Siti Nor Hawanis Husain , Ahmad Anas Nagoor Gunny

Food safety and quality are essential concerns for consumers worldwide. One way to ensure that the food we consume meets these standards is through proper food regulation. While there are various aspects of food regulation, one critical aspect is the regulations for food packaging materials. As such, this book chapter has been written to provide an in-depth overview of the regulations for food packaging materials in different continents, namely Asia, Europe, and America. The chapter delves into several countries in each continent, such as Malaysia, Japan, Australia, China, the United States, and South Africa, and discusses their specific food packaging regulations. This chapter also emphasizes the importance of considering the composition, migration properties, and potential for contamination of food packaging materials. It highlights the need for regulations to ensure that food packaging materials do not adversely affect the safety and quality of food products. In light of the evolving food packaging industry, the chapter further underscores the need for continuous evaluation and improvement of food packaging regulations. This is crucial to keep up with emerging risks and new technologies that may arise and impact the safety and quality of food.

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Studies on the third generation of Ionic liquids-Halophillic Cellulase system for Lignocellulose hydrolysis

2015 , Ahmad Anas Nagoor Gunny

The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end products. The limiting factor of these three processes lies in the pre-treatment steps. Conventional pre-treatment methods normally use strong acids or alkali which pose environmental problems. Recently pre-treatment using the third generation of Ionic Liquids (ILs), also known as Deep Eutectic Solvents (DESs) has been considered green and cost-effective. However, the main drawback of DESs pre-treatment is that it is not fully compatible with the enzyme required in saccharification. This compatibility issue was addressed in the present study by producing a salt tolerant enzyme (halophilic enzyme) and formulating low viscosity, thermal stable DES. The halophilic enzyme was produced from a newly isolated halophilic fungus, identified as Aspergillus terreus UniMAP AA-6; DESs were synthesized and screened using choline chloride as a hydrogen bond acceptor and selected carboxylic acids and polyol alcohols as hydrogen bond donors. The compatibility between halophilic cellulase and DESs was evaluated by monitoring the stability of halophilic cellulase in the presence of various concentrations of commercial ILs and DESs. The applicability of the DES-cellulase system for lignocelluloses hydrolysis was evaluated based on glucose production, energy consumption and kinetic performance. It was found that halophilic cellulase showed higher stability in the presence of 10% (v/v) ILs and also was stable and retained 90 % of its original activity in the presence of 10% (v/v) DESs. The DESs- Cellulase system exhibited higher glucose percentage enhancement and lower energy consumption as compared to diluted alkali system, while in terms of kinetic performance, DES exhibited good kinetic performance, which reflects the ability of DESs to serve as good saccharification media for the DESs-cellulase system. Finally, with regard to glucose production, rice husk treated with DESs-halophilic cellulase system were as good as the typical IL pre-treatment method but with extra benefits in terms of cost and environmental aspects. These findings have demonstrated a better approach for the in situ saccharification of DESs pre-treated lignocelluloses.

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Environmental Lighting towards Growth Effect Monitoring System of Plant Factory using ANN

2025-01-01 , Kader M.M.M.A. , Mansor M.N. , Razali Z.B. , Mustafa W.A. , Ahmad Anas Nagoor Gunny , Setumin S. , Osman M.K. , Idris M. , Akbar M.F. , Farid W.M.F.N.M. , Zainol M.Z. , Mizam N.S.S.

Malaysia is currently driven to become another most developed country in the world. Among other priority sector is Food Sustainability. Along the process, our vegetable supply-demand keeps increasing by year. Compared to traditional systems, closed systems or its other name called hydroponic is getting more important for plant production, with artificial light which has many potential advantages, including better quality transplants, shorter production time and less resource use. To gain full profit from it, the quality of vegetables needs to be controlled efficiently. Climate conditions, especially temperature and light intensity, have a significant impact on vegetable growth and yield, as well as nutritional quality. Plant growth and development are influenced by a variety of environmental factors, the most important one is light intensity. Among the problems to be tackled in this research are plant growth manual observation, light intensity variation and abundance of growth-related data to be evaluated manually. Therefore, to solve these problems, the specific type of vegetable used here is lettuce. The proposed methods are, observation of plant growth conducted automatically round the clock in intervals of 15 minutes for the whole month (estimated mature period of lettuce), using images captured. At the same time, the proposed light intensity which is red & white to the ratio of 2:1 (optimum ratio recommended by previous researchers) will be used. The issue of data to be evaluated manually will be solved using Artificial Neural Network (ANN) architecture, in specific Deep Learning. Concisely, the results & analysis shows the research is successfully developed for plant growth monitoring by using artificial neural network which, reached 80% to 90% accuracy in the training and validation session that made the architecture sufficient for determining the growth of the said vegetable. This is indeed foreseen, will highly assist the farmer in better monitoring the growth rate of the plant.

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Environmental lighting towards growth effect monitoring system of plant factory using ANN

2025-01 , Mohamed Mydin Hj M.Abdul Kader , Muhammad Naufal Mansor , Wan Azani Wan Mustafa , Ahmad Anas Nagoor Gunny

Malaysia is currently driven to become another most developed country in the world. Among other priority sector is Food Sustainability. Along the process, our vegetable supply-demand keeps increasing by year. Compared to traditional systems, closed systems or its other name called hydroponic is getting more important for plant production, with artificial light which has many potential advantages, including better quality transplants, shorter production time and less resource use. To gain full profit from it, the quality of vegetables needs to be controlled efficiently. Climate conditions, especially temperature and light intensity, have a significant impact on vegetable growth and yield, as well as nutritional quality. Plant growth and development are influenced by a variety of environmental factors, the most important one is light intensity. Among the problems to be tackled in this research are plant growth manual observation, light intensity variation and abundance of growth-related data to be evaluated manually. Therefore, to solve these problems, the specific type of vegetable used here is lettuce. The proposed methods are, observation of plant growth conducted automatically round the clock in intervals of 15 minutes for the whole month (estimated mature period of lettuce), using images captured. At the same time, the proposed light intensity which is red & white to the ratio of 2:1 (optimum ratio recommended by previous researchers) will be used. The issue of data to be evaluated manually will be solved using Artificial Neural Network (ANN) architecture, in specific Deep Learning. Concisely, the results & analysis shows the research is successfully developed for plant growth monitoring by using artificial neural network which, reached 80% to 90% accuracy in the training and validation session that made the architecture sufficient for determining the growth of the said vegetable. This is indeed foreseen, will highly assist the farmer in better monitoring the growth rate of the plant.

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