Ahmad Mukhlis Abdul Rahman
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Preferred name
Ahmad Mukhlis Abdul Rahman
Official Name
Ahmad Mukhlis, Abdul Rahman
Alternative Name
Abdul Rahman, Ahmad Mukhlis
Main Affiliation
Researcher ID
HGA-7390-2022

Research Output
Now showing 1 - 4 of 4
  • Publication
    Isolation of cellulolytic microorganism from food waste-fertilized soil
    (AIP Publishing Ltd., 2023)
    Aliaa Rasyidina Idrus
    ;
    Nor Hidayah Abd Aziz
    ;
    Zarina Zakaria
    ;
    Ahmad Mukhlis Abdul Rahman
    Uncontrolled disposal of waste from cellulosic materials can add burden to the existing environmental crises. The aim of the study is to isolate microorganisms from food waste-fertilized soil and identification of the microorganism species by genotyping. Soil samples enriched with fruits, rice and vegetables wastes were collected, diluted and spread on agar supplemented with carboxymethylcellulose (CMC) for induction of growth and cellulase production. Following screening of cellulolytic activity, isolates with significant cellulolytic index (CI) were chosen for molecular identification by PCR, using the 16S rRNA as target sequence in combination with in silico analysis. Four out of eleven microorganisms isolated from fruits and vegetable-rich soils produced a CI ranging from 0.43 to 2.00. Of these, only two isolates produced clear bands on PCR with approximately 1500 bp in size. In silico analysis of the isolates suggested species of Ochrobactrum sp. and Bacillus sp. These microorganisms have the potential to be used for decomposition of the cellulosic wastes.
  • Publication
    Loop-mediated isothermal amplification of the toxR gene coupled with lateral flow dipstick (LAMP-LFD) for the novel, rapid and specific visual detection of Vibrio harveyi
    (Malaysian Society for Microbiology, 2023)
    Ahmad Mukhlis Abdul Rahman
    ;
    Julian Ransangan
    ;
    Vijay Kumar Subbiah
    Vibrio harveyi is a serious pathogen for marine organisms particularly in hatcheries and grow-out ponds that attack their immune system. The rapid detection of V. harveyi is urgently needed to prevent bacterial spread. Here we described a rapid and specific visual detection method based on the Loop-Mediated Isothermal Amplification in combination with Lateral Flow Dipstick (LAMP-LFD). Methodology and results: A set of six novel primers were designed to target the toxR gene. These include the biotin-labelled inner primer that complements specifically to the target sequences. The resulting biotinylated LAMP amplicons were hybridised to the FAM-labelled probe resulting in lateral flow detection on the dipstick. The addition of loop primers improved the reaction time of LAMP by more than half and rapid detection was observed within 10-15 min. In comparison, the sensitivity of PCR-UV analysis was only at 104 copies while LAMP-LFD was able to detect lower amounts at 103 copies. The LFD provided higher specificity and selectivity since hybridization with specific probes to the LAMP amplicons was employed. In addition, detection of V. harveyi infected grouper was successful using the LAMP-LFD method described here. Conclusion, significance and impact of study: LAMP-LFD is specific to V. harveyi. Our method provides a useful tool to rapidly detect and monitor the outbreaks of the pathogen.
  • Publication
    Chloroplast DNA sequence of trnR-N and trnL-F regions in Harumanis mango from different orchards in Perlis, Malaysia
    (IOP Publishing Ltd, 2020)
    Ahmad Mukhlis Abdul Rahman
    ;
    SFM Sabri
    ;
    Yusuf, Arbai
    ;
    Zarina Zakaria
    ;
    SV Kumar
    Harumanis is a premium mango cultivar widely known for its sweet taste, aroma and vibrant flesh colour. To date, the genetic identification of this mango based on multiple conserved DNA region using samples from different orchards has never been reported. The aim of this research is to identify the genetic signature of Harumanis mango at molecular level by analyzing chloroplast DNA sequences of the trnL-F and trnR-N regions. DNA samples were extracted from a total of 15 Harumanis samples collected from five selected orchards using Cetyl Trimethyl Ammonium Bromide (CTAB) extraction procedure. The extracted DNA and the PCR-amplified products were analyzed through gel electrophoresis and were then subjected to DNA Sequencing and in silico analysis. The obtained sequences were compared with the sequences available in the GeneBank. BLAST search for both the trnR-N and trnL-F regions confirmed that all the 15 samples belong to Mangifera indica with a 99% sequence identity. In addition, the trnL-F sequences were 99% identical to a number of specific mango cultivars such as, Tommy and Arunika. However, the trnR-N sequences were less informative as it gave hits to only two mango accessions (e.g. Mangifera indica voucher PDBK 2014-0249). It is postulated that the plastid trnR-N may be a potential candidate region for the development of the Harumanis genetic signature. The results may be used to complement other molecular data for the development of a genetic barcode for Harumanis.
      1  16
  • Publication
    DNA barcoding of common Malaysia spiders using the Cytochrome Oxidase I (COI) gene
    (The Malaysian Society of Applied Biology, 2020)
    Ahmad Mukhlis Abdul Rahman
    ;
    Kek Chian Koay
    ;
    Johan Ariff Mohtar
    ;
    Anwardi Jamil
    ;
    Mohd Mushahril Abdul Shukor
    ;
    Nurul Ain Harmiza Abdullah
    For the last twenty years, many newly described spiders were collected from Malaysia and in fact, more than 11,000 species were recorded in Peninsular Malaysia as well as in Sabah and Sarawak states. Scientists have put an immense effort on untangling the spider biology from its physical structure and behavior to silks and venoms. However, working with spiders is impeded by the difficulties in species identification via solely morphological methods. Thus, DNA barcoding is an alternative technique that employs standard fragment to facilitate species identification. Isolation of genomic DNA from three Malaysian spiders were performed using NucleoSpin® DNA insect extraction kit. Amplification of reference mitochondrial cytochrome oxidase I (COI) gene employing PCR with two set of primers followed by the DNA sequencing and validation through phylogenetic analysis were carried out. The commercial extraction kit was effective for the recovery of good quality of intact genomic DNA band as indicated by the integrity analysis. Both set of primers successfully amplified 100% of the samples with approximately 600 – 700 bp of PCR products. The obtained sequences (610 bp to 692 bp) were compared with the sequences available in Gene Bank. BLAST and phylogenetic analysis revealed that the analyzed individual samples belong to Nephila pilipes, Neoscona nautica and Crossopriza lyoni, respectively. Phylogenetic analysis provided unique insight into the evolutionary relationship of each analyzed sample. This study aids in an accurate identification of the selected local spider species at molecular level using the COI gene.
      1  7