Ruslinda A. Rahim
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Ruslinda A. Rahim
Official Name
Ruslinda, A. Rahim
Alternative Name
Rahim, R. A.
Ruslinda, A. Rahim
A. Rahim, Ruslinda
Rahim Ruslinda, A.
Ruslinda, Abdul Rahim
Rahim, Ruslinda Abdul
Ruslinda, A. R.
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57194735807

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Field-Effect Transistor-based Biosensor Optimization: Single Versus Array Silicon Nanowires Configuration

2020-01-01 , Ong C.C. , Mohamad Faris Mohamad Fathil , Mohd Khairuddin Md Arshad , Mohammad Nuzaihan Md Nor , Ruslinda A. Rahim , Uda Hashim , Rafizatul Fitri Abdullah , Mohd Hazmi Mohd Ghazali , Tamjis N.

This paper demonstrated the effect of different number of silicon nanowire transducer channels, in other word single, double, and triple channels towards the performance of field-effect transistor-based biosensor through simulation tool. These silicon nanowire field-effect transistor biosensors were designed and simulated in device simulation modelling tool, Silvaco ATLAS with fixed length, width, and height of the silicon nanowire. Different negatively interface charge density values were applied on the transducer channels’ surface of the biosensors to represent as detected target biomolecules that will bind onto the surface of the transducer regions. Based on the results, more negatively interface charges density values presented on the sensing channels had reduced the electron carrier accumulation at the channel’s interface, therefore, reduced drain current flow between the source and drain terminal. With the increase number of the transducer channels, significant change in drain current for every applied negatively interface charges became more apparent and increased the sensitivity of the biosensor. The triple transducer channels silicon nanowire field-effect transistor biosensor had demonstrated highest sensitivity, that is 2.83 µA/e∙cm2, which indicates it has better response for the detection of interface charges, thus increases chances for transducer channels reaction to the target biomolecules during testing or diagnosis.

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Fabrication of Graphene Electrode via Graphene Transfer Method for Bisphenol A (BPA) Detection

2021-01-01 , Shukri N.I.B.A. , Norhayati Sabani , Ruslinda A. Rahim , Mohamad Faris Mohamad Fathil , Syarifah Norfaezah Sabki , Nur Hamidah Abdul Halim , Nur Syakimah Ismail

Exposure of BPA is a concern as BPA can seep into food or beverages from containers and can possibly effects on human health especially endocrine systems. An electrochemical-based aptasensor utilizing graphene was developed in detecting endocrine disrupting compound Bisphenol A (BPA, 4,4'-(propane-2,2-diyl) diphenol). The graphene modified electrode was developed via graphene transfer. Fabrication and characterization of graphene transfer was studied in this paper using Scanning Electron Microscopy (SEM) and High-Power Microscope (HPM). In this research, the investigation of interfacial characteristic modified graphene with aptasensor and recognition of BPA with aptasensor had been done using electrochemical impedance spectroscopy (EIS). The increment of charge transfer resistance (Rct) before and after recognition of BPA denoting the accumulation of charge at the electrode surface in this research.

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Surface Morphology Analysis of graphene transfer on SiO2 with BPA aptasensor detection using Electrochemical Impedance Spectroscopy

2023-01-01 , Shukri N.I.A. , Norhayati Sabani , Mohamad Faris Mohamad Fathil , Syarifah Norfaezah Sabki , Ruslinda A. Rahim , Halim N.H.A. , Ismail N.S.

Bisphenol A or BPA is one of the highest produced chemicals in the world. The production of polycarbonate plastic and epoxy resin are used to make variety of consumer goods and it is frequently employed BPA as a raw material. BPA is one of the endocrine disruptors which is related to a wide range of adverse health effects that can cause reproductive disorders and many kinds of cancers. In the work, the novelty of electrochemical sensor of BPA was constructed on a graphene modified electrode using graphene transfer method. In this work, High-power microscope and scanning electron microscopy were used to study the production and characterization of the graphene, with two significant mapping graphene at 20% and 80%. The existence of graphene on silicon oxide was analyzed using Raman Spectroscopy while the composition of the materials was analyze using Fourier-Transform Infrared Spectroscopy. In this analysis, both analysis data from Raman and FTIR clearly shown that 80% mapping graphene is the best option which resulting to the high surface coverage. The electrochemical performance of the mapping 80% graphene electrode was examined using Electrochemical Impedance Spectra. The increase in charge transfer resistance (Rct) both before and after the addition of BPA denotes the development of the charge at the electrode surface. The equivalent circuit shows the Rct of graphene increased from 0.4 k Ω to 1.2 k Ω and drastically increased to 300 kΩ when the device was introduced with BPA due to the existence of a negative charge carrier and the repelling contact.

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Ultrasensitive and highly selective graphene-based field-effect transistor biosensor for anti-diuretic hormone detection

2020-05-01 , Selvarajan, Reena Sri , Ruslinda A. Rahim , Burhanuddin Yeop Majlis , Subash Chandra Bose Gopinath , Azrul Azlan Hamzah

Nephrogenic diabetes insipidus (NDI), which can be congenital or acquired, results from the failure of the kidney to respond to the anti-diuretic hormone (ADH). This will lead to excessive water loss from the body in the form of urine. The kidney, therefore, has a crucial role in maintaining water balance and it is vital to restore this function in an artificial kidney. Herein, an ultrasensitive and highly selective aptameric graphene-based field-effect transistor (GFET) sensor for ADH detection was developed by directly immobilizing ADH-specific aptamer on a surface-modified suspended graphene channel. This direct immobilization of aptamer on the graphene surface is an attempt to mimic the functionality of collecting tube V2 receptors in the ADH biosensor. This aptamer was then used as a probe to capture ADH peptide at the sensing area which leads to changes in the concentration of charge carriers in the graphene channel. The biosensor shows a significant increment in the relative change of current ratio from 5.76 to 22.60 with the increase of ADH concentration ranging from 10 ag/mL to 1 pg/mL. The ADH biosensor thus exhibits a sensitivity of 50.00 µA·(g/mL)−1 with a limit of detection as low as 3.55 ag/mL. In specificity analysis, the ADH biosensor demonstrated a higher current value which is 338.64 µA for ADH-spiked in phosphate-buffered saline (PBS) and 557.89 µA for ADH-spiked in human serum in comparison with other biomolecules tested. This experimental evidence shows that the ADH biosensor is ultrasensitive and highly selective towards ADH in PBS buffer and ADH-spiked in human serum.

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Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer

2023-07 , F. Nadhirah Jaapar , Nor Azizah Parmin , Nur Hamidah Abdul Halim , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Sh. Nadzirah , Voon Chun Hong , Muhammad Nur Aiman Uda , Wei Chern Ang , Iffah Izzati Zakaria , Zulida Rejali , Amilia Afzan , Azrul Azlan Hamzah , Chang Fu Dee , F. Syakirah Halim

For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.

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Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

2020-04 , Nurul Atiqah Ahmad , Ruslinda A. Rahim , Bohuslav Rezek , Alexander Kromka , Nur Syakimah Ismail , Subash Chandra Bose Gopinath , Tibor Izak , Vaclav Prochazka , Fatin Nabilah Mohd Faudzi , Azrul Syafiq Zainol Abidin , Nur Nasyifa Mohd Maidizn

Nanocrystalline diamonds have recently gained great attention to circumvent the current hurdles, with their appealing properties such as high-surface-area to volume ratio, low-background current, wide potential window, biocompatibility, and chemical stability. The nanocrystalline diamonds electrolyte-gated field-effect transistor (NCD-EGFET) can operate directly in solution without involving gate oxides in bringing the hydrogen-tethered moieties and facilitates the p-type surface conductivity. This research investigated on Trans-activator of transcription (Tat) protein; a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis.

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Electrical simulation on silicon nanowire field-effect transistor biosensor at different substrate-gate voltage bias conditions for charge detection

2022-12 , X.Y. Teoh , Mohamad Faris Mohamad Fathil , Y.M.Tan , Norhayati Sabani , Mohammad Nuzaihan Md Nor , Mohd Khairuddin Md Arshad , Ruslinda A. Rahim , Nur Hamidah Abdul Halim , Ramzan Mat Ayub , Uda Hashim , M.M. Ibrahim

In this work, the impact of different substrate-gate voltage bias conditions (below and above the device threshold voltage) on current-voltage characteristics and sensitivity of a silicon nanowire field-effect transistor (SiNW-FET) biosensor was investigated. A 3-dimensional device structure with n-type SiNW channel and a substrate gate electrode was designed and electrically simulated In the Silvaco ATLAS. Next, the SiNW channel was covered with a range of interface charge density to mimic the charged target biomolecule captured by the device. The outcome was translated into a drain current versus interface charge semi-log graph and the device sensitivity was calculated using the linear regression curve’s slope of the plotted data. The device’s electrical characteristic shown higher generation of output drain current values with the increase of negative substrate-gate voltage bias due to the hole carriers’ accumulation that forms a conduction channel in the SiNW. Application of higher negative interface charge density increased the change in drain current, with the device biased with higher substrate-gate voltage shows more significant change in drain current. The device sensitivity increased when biased with higher substrate-gate voltage with highest sensitivity is 75.12 nA/dec at substrate-gate voltage bias of –1.00 V.

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Facile Electrical DNA Genosensor for Human Papillomavirus (HPV 58) for Early Detection of Cervical Cancer

2023-07-01 , Jaapar F.N. , Nor Azizah Parmin , Halim N.H.A. , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Nadzirah S. , Voon Chun Hong , Muhammad Nur Aiman Uda , Ang W.C. , Zakaria I.I. , Rejali Z. , Afzan A. , Hamzah A.A. , Dee C.F. , Halim F.S.

For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10µM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.

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Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

2020-12-01 , Nur Nasyifa M.M. , Ruslinda A. Rahim , Nur Hamidah Abdul Halim , Zainol Abidin A.S. , Mohd Faudzi F.N. , Ahmad N.A. , Lockman Z. , Rezek B. , Kromka A. , Subash Chandra Bose Gopinath

Physiological and emotional stress affects the regulation of cortisol secretion, a routine process in circadian rhythm. Regular monitoring of cortisol level as a biomarker in the blood stream becomes vital to determine cortisol-related diseases. This study reports immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor (EGFET) biosensor for cortisol determination in human serum. Solution-processed graphene nanoplatelets were evidenced on the surface by Raman spectroscopy analysis and utilized as the transducing element on the field-effect transistor. Further, confirmed the binding events of the antibody on graphene nanoplatelets using X-Ray Photoelectron Spectroscopy and characterized the electrostatic gating effect of cortisol and intermediate functionalization on graphene nanoplatelets-EGFET. The biosensor exhibited good sensitivity of 72.30 µA.(g/mL)−1 in a linear range between 1.00 pg/mL to 10.00 ng/mL, with a limit of detection (LOD) of 0.85 pg/mL. Confirmation with binding events on the biosensor was done using the relevant molecules, progesterone, cortisone, and corticosterone, and found to be selective towards cortisol. Cortisol was also successfully detected with interference by the human serum, suggesting the capability of graphene nanoplatelets-EGFET sensor for determining cortisol in a complex matrix.

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Formation of polypropylene nanocomposite joint using silicon carbide nanowhiskers as novel susceptor for microwave welding

2023-05-01 , Foong P.Y. , Voon Chun Hong , Lim B.Y. , Teh P.L. , Mohd Afendi Rojan , Subash Chandra Bose Gopinath , Mohd Khairuddin Md Arshad , Nor Azizah Parmin , Low F.W. , Ruslinda A. Rahim , Uda Hashim

Up to present, no study has reported on the use of silicon carbide nanomaterials (SiCNMs) as susceptor for microwave welding of thermoplastics. Therefore, in this study, silicon carbide nanowhiskers (SiCNWs) was attempted as the microwave susceptor for the microwave welding of polypropylene (PP). It was observed that SiCNWs are capable of absorbing microwave and converting them into heat, leading to a sharp increase in temperature until it reaches the melting point of PP substrates. The microwave welded joint is formed after the molten PP at the interface between PP substrates is cooled under pressure. The effect of microwave heating duration and solid loading of SiCNWs suspension was studied and reported. The formation mechanism of SiCNWs reinforced PP welded joint was proposed in this study. With these remarkable advantages of microwave welding and enhanced mechanical properties of the welded joint, it is believed that this study can provide a new insight into welding of thermoplastic and material processing through short-term microwave heating.

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