Ruslinda A. Rahim
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Ruslinda A. Rahim
Official Name
Ruslinda, A. Rahim
Alternative Name
Rahim, R. A.
Ruslinda, A. Rahim
A. Rahim, Ruslinda
Rahim Ruslinda, A.
Ruslinda, Abdul Rahim
Rahim, Ruslinda Abdul
Ruslinda, A. R.
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57194735807

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Measurement of rice moisture content based on quantitative analysis from radio tomography images

2024-05-01 , Nurul Amira Mohd Ramli , Mohd Hafiz Fazalul Rahiman , Ruslinda A. Rahim , Latifah Munirah Kamarudin , Latifah Mohamed , Moqbel Abdullah M.S. , Ammar Zakaria

Inefficient storage of paddy and rice grains can lead to grain deterioration, resulting in post-harvest losses ranging from 10% to 30%. The quality of grains cannot be improved throughout the storage period. Therefore, following the mechanisation of agricultural industries, air dryers have been developed to control the crops’ moisture level by blowing ambient or heated air into the silo to improve the aeration and allow the grains to be preserved with minimal loss of quality until the appropriate time for managing and marketing processes. However, the conventional sampling method used to measure the moisture level is inefficient because it is very localised and only represents part of the moisture distribution inside the bulk grains. Additionally, incorporating advanced technologies can be a significant cost limitation for small-scale industries. Thus, to address the issue, this research study developed a radio tomographic imaging (RTI) system in a silo-scale prototype using 20 sensor nodes operating at 2.4 GHz to localise and monitor the moisture level constructively. The RTI system reconstructs the cross-sectional images across the rice silo by measuring radio frequency attenuation, in terms of received signal strength (RSS) quality, caused by the rice moisture phantoms within the wireless sensor network (WSN) area. A total of five phantoms’ profiles having a percentage of moisture content (MC)of 15%, 20% and 25% were reconstructed using four image reconstruction algorithms,Linear Back Projection (LBP), Filtered Back Projection (FBP), Newton’s One-step ErrorReconstruction (NOSER) and Tikhonov Regularisation. Then, an image quality assessment,Mean Structural Similarity Index (MSSIM), was utilised to evaluate the performance of thereconstructed images. Lastly, a numerical method based on the first-order linear regressionmodel was introduced as a preliminary approach toward the method’s establishment. In summary, the experimental results demonstrated average image quality scores for all MClevels (15%, 20% and 25%), where the range scores are 0.2776 – 0.4755. Based on thenumerical analysis, the results support the possibility of engaging the proposed techniqueto monitor the moisture level inside a rice silo with the highest and lowest correlationcoefficients of 0.7218 and 0.5442, respectively.

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Facile Electrical DNA Genosensor for Human Papillomavirus (HPV 58) for Early Detection of Cervical Cancer

2023-07-01 , Jaapar F.N. , Nor Azizah Parmin , Halim N.H.A. , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Nadzirah S. , Voon Chun Hong , Muhammad Nur Aiman Uda , Ang W.C. , Zakaria I.I. , Rejali Z. , Afzan A. , Hamzah A.A. , Dee C.F. , Halim F.S.

For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10µM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.

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Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

2020-12-01 , Nur Nasyifa M.M. , Ruslinda A. Rahim , Nur Hamidah Abdul Halim , Zainol Abidin A.S. , Mohd Faudzi F.N. , Ahmad N.A. , Lockman Z. , Rezek B. , Kromka A. , Subash Chandra Bose Gopinath

Physiological and emotional stress affects the regulation of cortisol secretion, a routine process in circadian rhythm. Regular monitoring of cortisol level as a biomarker in the blood stream becomes vital to determine cortisol-related diseases. This study reports immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor (EGFET) biosensor for cortisol determination in human serum. Solution-processed graphene nanoplatelets were evidenced on the surface by Raman spectroscopy analysis and utilized as the transducing element on the field-effect transistor. Further, confirmed the binding events of the antibody on graphene nanoplatelets using X-Ray Photoelectron Spectroscopy and characterized the electrostatic gating effect of cortisol and intermediate functionalization on graphene nanoplatelets-EGFET. The biosensor exhibited good sensitivity of 72.30 µA.(g/mL)−1 in a linear range between 1.00 pg/mL to 10.00 ng/mL, with a limit of detection (LOD) of 0.85 pg/mL. Confirmation with binding events on the biosensor was done using the relevant molecules, progesterone, cortisone, and corticosterone, and found to be selective towards cortisol. Cortisol was also successfully detected with interference by the human serum, suggesting the capability of graphene nanoplatelets-EGFET sensor for determining cortisol in a complex matrix.

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Designing DNA probe from human Papillomavirus (HPV) 58 in E6 region as biosensing element for development of biosensor

2024-03-21 , Jaapar N.F. , Nor Azizah Parmin , Halim N.H.A. , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Nadzirah S. , Voon Chun Hong , Muhammad Nur Aiman Uda , Ang W.C. , Zakaria I.I. , Rejali Z. , Afzan A. , Hamzah A.A. , Dee C.F. , Halim F.S.

Globally, second leading cause of death for women is a Cervical Cancer. CC is caused by infection of Human Papillomavirus (HPV). HPV strains 16 (50.8%), 18 (17.6%), and 58 (2.6%) became the most leading strains of infection in Malaysia. Recently, a study showed that HPV 58 was rare worldwide but famous in Asia countries including Malaysia. However, detection the significance of HPV-58 in women has not been studied extensively because of rare case compared to HPV 16 and 18. HPV-58 is commonly found in East Asia, but infrequently worldwide, due to changes in the environment of viruses and humans. Detailed biological knowledge is crucial for the development of effective countermeasures, diagnostic tests, vaccines and antiviral drugs against the HPV. The oligonucleotide sequences of HPV 58 in E6 region have been analysed between 24-35 mer in order to maintain the specificity and selectivity. The percentage of similarities between the coding sequences has developed with 66.7% of GC content. The DNA probe of HPV 58 was 5'GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3' with 30 mer of oligonucleotides. The important of E6 region for developing the coding sequence as it involved in the DNA reproduction, transcription, translation regulation and transformation of HPV genome. Phylogenetic trees were then constructed by Neighbour-Joining and the Kimura 2-parameters methods, followed by an analysis of selection pressures acting on the E6/E7 genes by ebi ac uk tools.

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Field-Effect Transistor-based Biosensor Optimization: Single Versus Array Silicon Nanowires Configuration

2020-01-01 , Ong C.C. , Mohamad Faris Mohamad Fathil , Mohd Khairuddin Md Arshad , Mohammad Nuzaihan Md Nor , Ruslinda A. Rahim , Uda Hashim , Rafizatul Fitri Abdullah , Mohd Hazmi Mohd Ghazali , Tamjis N.

This paper demonstrated the effect of different number of silicon nanowire transducer channels, in other word single, double, and triple channels towards the performance of field-effect transistor-based biosensor through simulation tool. These silicon nanowire field-effect transistor biosensors were designed and simulated in device simulation modelling tool, Silvaco ATLAS with fixed length, width, and height of the silicon nanowire. Different negatively interface charge density values were applied on the transducer channels’ surface of the biosensors to represent as detected target biomolecules that will bind onto the surface of the transducer regions. Based on the results, more negatively interface charges density values presented on the sensing channels had reduced the electron carrier accumulation at the channel’s interface, therefore, reduced drain current flow between the source and drain terminal. With the increase number of the transducer channels, significant change in drain current for every applied negatively interface charges became more apparent and increased the sensitivity of the biosensor. The triple transducer channels silicon nanowire field-effect transistor biosensor had demonstrated highest sensitivity, that is 2.83 µA/e∙cm2, which indicates it has better response for the detection of interface charges, thus increases chances for transducer channels reaction to the target biomolecules during testing or diagnosis.

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Fabrication of Graphene Electrode via Graphene Transfer Method for Bisphenol A (BPA) Detection

2021-01-01 , Shukri N.I.B.A. , Norhayati Sabani , Ruslinda A. Rahim , Mohamad Faris Mohamad Fathil , Syarifah Norfaezah Sabki , Nur Hamidah Abdul Halim , Nur Syakimah Ismail

Exposure of BPA is a concern as BPA can seep into food or beverages from containers and can possibly effects on human health especially endocrine systems. An electrochemical-based aptasensor utilizing graphene was developed in detecting endocrine disrupting compound Bisphenol A (BPA, 4,4'-(propane-2,2-diyl) diphenol). The graphene modified electrode was developed via graphene transfer. Fabrication and characterization of graphene transfer was studied in this paper using Scanning Electron Microscopy (SEM) and High-Power Microscope (HPM). In this research, the investigation of interfacial characteristic modified graphene with aptasensor and recognition of BPA with aptasensor had been done using electrochemical impedance spectroscopy (EIS). The increment of charge transfer resistance (Rct) before and after recognition of BPA denoting the accumulation of charge at the electrode surface in this research.

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Dielectrophoresis as an adjunctive technique for fibroblast cell migration to enhance wound closure

2024-06 , Nur Nasyifa Mohd Maidin , Revathy Deivasigamani , Muhamad Ramdzan Buyong , Mohd Ambri Mohamed , Ruslinda A. Rahim

This study reports on DEP-based simulation and experimental validation of polystyrene (PS) beads and fibroblast cells for primary skin cell migration for enhancing wound closure. MyDEP software was used to calculate the numerical simulation of the Clausius-Mossotti factor (CMF). In order to examine particle trajectories based on input frequencies, the finite element technique (FEM) is used. The trajectories of PS beads and fibroblast cells were experimentally assessed to verify the impact of frequency applied on the polarisation of PS beads and fibroblast cells. The outcome illustrated the potential of employing FDEP to move particles and cells to regions of high and low electric field. Fibroblast cells exhibit negative dielectrophoresis (NDEP) at a broad range of frequencies. Thus, FDEP can be utilised for frequency optimisation to enhance wound closure.

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Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer

2023-07 , F. Nadhirah Jaapar , Nor Azizah Parmin , Nur Hamidah Abdul Halim , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Sh. Nadzirah , Voon Chun Hong , Muhammad Nur Aiman Uda , Wei Chern Ang , Iffah Izzati Zakaria , Zulida Rejali , Amilia Afzan , Azrul Azlan Hamzah , Chang Fu Dee , F. Syakirah Halim

For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.

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Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface

2020-04 , Nurul Atiqah Ahmad , Ruslinda A. Rahim , Bohuslav Rezek , Alexander Kromka , Nur Syakimah Ismail , Subash Chandra Bose Gopinath , Tibor Izak , Vaclav Prochazka , Fatin Nabilah Mohd Faudzi , Azrul Syafiq Zainol Abidin , Nur Nasyifa Mohd Maidizn

Nanocrystalline diamonds have recently gained great attention to circumvent the current hurdles, with their appealing properties such as high-surface-area to volume ratio, low-background current, wide potential window, biocompatibility, and chemical stability. The nanocrystalline diamonds electrolyte-gated field-effect transistor (NCD-EGFET) can operate directly in solution without involving gate oxides in bringing the hydrogen-tethered moieties and facilitates the p-type surface conductivity. This research investigated on Trans-activator of transcription (Tat) protein; a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis.

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Surface Morphology Analysis of graphene transfer on SiO2 with BPA aptasensor detection using Electrochemical Impedance Spectroscopy

2023-01-01 , Shukri N.I.A. , Norhayati Sabani , Mohamad Faris Mohamad Fathil , Syarifah Norfaezah Sabki , Ruslinda A. Rahim , Halim N.H.A. , Ismail N.S.

Bisphenol A or BPA is one of the highest produced chemicals in the world. The production of polycarbonate plastic and epoxy resin are used to make variety of consumer goods and it is frequently employed BPA as a raw material. BPA is one of the endocrine disruptors which is related to a wide range of adverse health effects that can cause reproductive disorders and many kinds of cancers. In the work, the novelty of electrochemical sensor of BPA was constructed on a graphene modified electrode using graphene transfer method. In this work, High-power microscope and scanning electron microscopy were used to study the production and characterization of the graphene, with two significant mapping graphene at 20% and 80%. The existence of graphene on silicon oxide was analyzed using Raman Spectroscopy while the composition of the materials was analyze using Fourier-Transform Infrared Spectroscopy. In this analysis, both analysis data from Raman and FTIR clearly shown that 80% mapping graphene is the best option which resulting to the high surface coverage. The electrochemical performance of the mapping 80% graphene electrode was examined using Electrochemical Impedance Spectra. The increase in charge transfer resistance (Rct) both before and after the addition of BPA denotes the development of the charge at the electrode surface. The equivalent circuit shows the Rct of graphene increased from 0.4 k Ω to 1.2 k Ω and drastically increased to 300 kΩ when the device was introduced with BPA due to the existence of a negative charge carrier and the repelling contact.

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