Nor Azizah Parmin
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Nor Azizah Parmin
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Nor Azizah, Parmin
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Parmin, N. A.
Parmin, Nor Azizah
Parmin, Nor A.
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Scopus Author ID
57195835481
Researcher ID
S-6303-2019

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The study of sensing elements parameters optimization for developed biosensor of SARS-CoV-2 detection

2023-04 , Fatin Syakirah Halim , Nor Azizah Parmin , Uda Hashim , Subash Chandra Bose Gopinath , Farrah Aini Dahalan , Iffah Izzati Zakaria , Wei Chern Ang , Nurfareezah Nadhirah Jaapar

New advancements in developing sensitive and selective biosensors have demonstrated outstanding potential for Deoxyribonucleic Acid (DNA biosensors). The detection mode of DNA biosensors primary depends on a particular DNA hybridization that precisely occurs on the surface of the physical transducer that can only be detected using high-performance assays due to slight current changes. The analytical performance (sensitivity) of the DNA biosensor is conclusively rely on the confluence constructing of the sensing surface, which must be optimized. Thus, in this study, the sensing elements of the developed biosensors were optimized for detecting RNA of SARS-CoV-2. This optimization included concentration of nanomaterials (carbon quantum dots), probe density (concentration of DNA probe) and concentration of linker (APTES). It was observed that 0.15 % V/V of concentration CQD, 0.1μM of DNA probe and 36% V/V of APTES were the optimum parameters which provided their maximum response during electrical measurements and increased the sensitivity of the developed biosensor for SARS-CoV-2 detection

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Potentials of MicroRNA in Early Detection of Ovarian Cancer by Analytical Electrical Biosensors

2022-01-01 , Nor Azizah Parmin , Uda Hashim , Subash Chandra Bose Gopinath , Nadzirah S. , Salimi M.N. , Voon Chun Hong , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Rozi S.K.M. , Rejali Z. , Afzan A. , Azan M.I.A. , Yaakub A.R.W. , Hamzah A.A. , Dee C.F.

The importance of nanotechnology in medical applications especially with biomedical sensing devices is undoubted. Several medical diagnostics have been developed by taking the advantage of nanomaterials, especially with electrical biosensors. Biosensors have been predominantly used for the quantification of different clinical biomarkers toward detection, screening, and follow-up the treatment. At present, ovarian cancer is one of the severe complications that cannot be identified until it becomes most dangerous as the advanced stage. Based on the American Cancer Society, 20% of cases involved in the detection of ovarian cancer are diagnosed at an early stage and 80% diagnosed at the later stages. The patient just has a common digestive problem and stomach ache as early symptoms and people used to ignore these symptoms. Micro ribonucleic acid (miRNA) is classified as small non-coding RNAs, their expressions change due to the association of cancer development and progression. This article reviews and discusses on the currently available strategies for the early detection of ovarian cancers using miRNA as a biomarker associated with electrical biosensors. A unique miRNA-based biomarker detections are specially highlighted with biosensor platforms to diagnose ovarian cancer.

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Designing specific SARS-CoV-2 DNA probe as biosensing element for development of biosensor

2024-03-21 , Halim F.S. , Nor Azizah Parmin , Uda Hashim , Subash Chandra Bose Gopinath , Dahalan F.A. , Zakaria I.I. , Ang W.C. , Jaapar N.F.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a part of the family of beta-coronaviruses inducing COVID-19 disease. COVID-19 became the most life-threatening and highly contagious viral disease compare to another disease family of coronavirus. Right now, the RT-PCR is the gold standard for the diagnostic of COVID-19. To produce successful prevention strategies, medical testing, vaccinations, and antiviral drugs against COVID-19, comprehensive biological information is critical. Conserved coding RNA sequence within the N gene region of the open reading frame in the SARS-CoV-2 genome was employed as the foundation for creating an oligonucleotide probe as it is a crucial component for the development of a biosensor to identify the virus. The study aimed to develop a DNA probe that complementary to the RNA target of the N region for SARS-CoV-2. Studies were conducted on various strains of coronavirus sequences to verify the percent of correlation as well as the region of consensus that triggers various strains of viruses. Basic local alignment search tools (BLAST) and CLUSTLW had devoted additional statistical parameters, for example, desire values (E-values) and score bits. The 30 mer DNA probe with 50.0% of GC content was developed, CTG AAG CGC TGG GGG CAA ATT GTG CAA TTT. The adequate length of the probe is between 22 and 31 mer. The complementary DNA probe was designed based on the RNA target from the N-region selection that has been identified that could be used as a biomarker probe to produce a biosensor that can be implemented to the clinical and environmental diagnosis of COVID-19.

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Electrochemical DNA Biosensor based on 30 nM Gold Nanoparticle Modified Electrode by Electro Less Deposition for Human Papillomavirus (HPV) 18 E6 Region

2020-07-09 , Koo Siew Kim N.S. , Nor Azizah Parmin , Uda Hashim , Subash Chandra Bose Gopinath , Rejali Z. , Afzan A. , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Voon Chun Hong

The aim of this work was to develop a novel, simple, inexpensive, sensitive an electrochemical DNA biosensor based on interdigitated electrodes (IDEs) integrated gold nanoparticle modified electrode by electro less deposition for HPV 18. The biosensor was designed with a 30 mer E6 region of HPV 18 DNA modified probe. The E6 region has been used for their clinical importance properties and suitable as recognition biomarker region. Three different target types were tested which complementary target, non-complementary target and mismatch target. All target were analyzed for detection of HPV 18 in early stages by using Dielectric Analyzer (DA), Alpha-A High-performance Frequency Analyzer, Novocontrol Technologies, Handsagen, Germany associated with the software package Windeta. Complementary target gives a positive result in HPV detection, while non-complementary and mismatch target give negative results. IDE device with 5 nm gap sizes has demonstrated a high performance towards the detection of HPV18 ssDNA target by modified with 30 nm gold nanoparticle. The electrochemical biosensor showed better performance compared to agarose gel electrophoresis assay. This technology can be used as a new and attractive sensor development for detection of virus infection in human bodies.

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Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer

2023-07 , F. Nadhirah Jaapar , Nor Azizah Parmin , Nur Hamidah Abdul Halim , Uda Hashim , Subash Chandra Bose Gopinath , Ruslinda A. Rahim , Sh. Nadzirah , Voon Chun Hong , Muhammad Nur Aiman Uda , Wei Chern Ang , Iffah Izzati Zakaria , Zulida Rejali , Amilia Afzan , Azrul Azlan Hamzah , Chang Fu Dee , F. Syakirah Halim

For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.

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Femtomolar Dengue Virus Type-2 DNA Detection in Back-gated Silicon Nanowire Field-effect Transistor Biosensor

2022-01-01 , Abidin W.A.B.Z. , Nor M.N.M. , Mohd Khairuddin Md Arshad , Mohamad Faris Mohamad Fathil , Nor Azizah Parmin , Sisin N.A.H.T. , Ibau C. , Azlan A.S.

Background: Dengue is known as the most severe arboviral infection in the world spread by Aedes aegypti. However, conventional and laboratory-based enzyme-linked immunosorbent as-says (ELISA) are the current approaches in detecting dengue virus (DENV), requiring skilled and well-trained personnel to operate. Therefore, the ultrasensitive and label-free technique of the Silicon Nanowire (SiNW) biosensor was chosen for rapid detection of DENV. Methods: In this study, a SiNW field-effect transistor (FET) biosensor integrated with a back-gate of the low-doped p-type Silicon-on-insulator (SOI) wafer was fabricated through conventional photo-lithography and Inductively Coupled Plasma – Reactive Ion Etching (ICP-RIE) for Dengue Virus type-2 (DENV-2) DNA detection. The morphological characteristics of back-gated SiNW-FET were examined using a field-emission scanning electron microscope supported by the elemental analysis via energy-dispersive X-ray spectroscopy. Results and Discussion: A complementary (target) single-stranded deoxyribonucleic acid (ssDNA) was recognized when the target DNA was hybridized with the probe DNA attached to SiNW surfaces. Based on the slope of the linear regression curve, the back-gated SiNW-FET biosensor demonstrated the sensitivity of 3.3 nAM-1 with a detection limit of 10 fM. Furthermore, the drain and back-gate voltages were also found to influence the SiNW conductance changed. Conclusion: Thus, the results obtained suggest that the back-gated SiNW-FET shows good stability in both biosensing applications and medical diagnosis throughout the conventional photolithography method.

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Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application

2024-06-01 , Adilah Ayoib , Karim N.A.A.A. , Uda Hashim , Shamsuddin S.A. , Abd Rahman S.F. , Mohamad Faris Mohamad Fathil , Nor Azizah Parmin

Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 µm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.

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Micro-interdigitated electrodes genosensor based on Au-deposited nanoparticles for early detection of cervical cancer

2023-12-31 , Jaapar F.N. , Nor Azizah Parmin , Halim N.H.A. , Uda Hashim , Subash Chandra Bose Gopinath , Halim F.S. , Uda M.N.A. , Afzan A. , Nor N.M. , Razak K.A.

Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.

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Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles

2022-10-01 , Jaapar F.N. , Nor Azizah Parmin , Halim N.H.A. , Uda Hashim , Subash Chandra Bose Gopinath , Halim F.S. , Ruslinda A.R. , Voon Chun Hong , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Nadzirah S. , Rejali Z. , Afzan A. , Zakaria I.I.

The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.

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Design and Fabrication of Multichannel PDMS Microfluidic

2021-12-14 , Muhammad Nur Aiman Uda , Uda Hashim , Muhammad Nur Afnan Uda , Nor Azizah Parmin , Thivina V.

Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC's. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.

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