Traditional medicinal plants are widely used to prevent or cure diseases in many countries all over the world. Malaysia, which is one of the countries, has provided advanced knowledge of traditional medicinal plants and herbs. Therefore, the present study was conducted in Malaysia to evaluate a comparison of antioxidant activity, the total phenolic and flavonoid content of the female and male leaves of Ficus de/to idea (Mas Cotek. ). It is one of the public and famous plants in Malaysia. The antioxidant activity was determined using free radical scavenging activity measured by DPPH 1, 1- diphenyl-2-picrylydrazyl and H202 hydrogen peroxide scavenging assay compared to Vitamin C as a standard. The total phenolic and flavonoid content (TPC and TFC) were then compared to determine which one has higher amount. The results of this showed that female leaves of F.de/toidea have higher antioxidant activity and higher TFC and TPC content. The antioxidants activity determined by the same test mentioned above was then evaluated using Gynocthodes sublanceo/ata individually and in combination with female leaves of F.deltoidea with G. sublanceolata (50%:50%). Cytotoxicity 3- (4,5 Dimethylthiozol-2-yl)-2,5-Diphenyletetrazolium (MTT assay) and morphological study of two medicinal plants individually and in combination. F. deltoidea leaves extract were indicated to have a higher antioxidant activity in DPPH and H20 2 scavenging assay TPC and TFC than G. sublanceolata leaves extract. The combination for these extracts is higher than from each one individually with all tests. The highest result indicated the combination for TPC and TFC at 170.12 ~g/mg and 51.19~g/mg,
respectively. For (MIT) assay, this test used two cancer cell lines such as Human Breast MCF-7, Prostatic PC3 adenocarcinoma and Human Umbilical vein endothelial cell line (HUVEC) as normal cell lines. The combination revealed the best inhibition on cancer cell lines and ignorable effect on normal cell lines compared to those work individually. The results indicated that the combination of plants was tested and exhibited a remarkable cytotoxic activity more than 63% at 200 ~g /ml. Individual inhibition on cancer cell lines was enhanced, whereas inhibition on normal cell lines was still less than that on cancer cell lines. PC3 for F.de/toidea ICso 100 ~g/mL, and G. sublanceolata was ICso 138 ~g/mL, and combination was ICso 83 ~g/mL. On MCF-7,
F.deltoidea IC50 125 ~g/mL G. sublanceolata was ICso 159 ~g/mL, and combination was IC50 95 ~g/mL. HUVEC cell line showed ICso 180 ~g/mL for F. deltoidea and that for G. sublanceo/ata ICso168 ~g/mL, but that for combination was IC50 196 ~g/mL. In addition, the plants extract showed a significant anti-proliferative effect MCF-7 and PC3 cancer cells individually, but indicated mild-cytotoxic to the tested HUVEC normal cells. However, for the combination of the herbs on normal cell lines have preserved while the cancer cell lines growth was inhibited. Two extract individually showed morphological changes on the ceJls. On the other hand, the combination showed more morphological changes on cancer cells.
