Nowadays, aquaculture is searching for a sustainable and eco-friendly water filtration system for their fish farming tank. This paper presents the use of biomass as the filtration layer in the fish tank. Biomass from fruit peels (apple peels, pear peels, and orange peels) was prepared to add as the second layer, while filter sponge cartridges as a primary filtration system for the fish farming tank. Tilapia fish, a significant farming fish species worldwide, was chosen as a fish source. The temperature was controlled between 20-35 °C because suitable for Tilapia fish. The pH value suitable for Tilapia fish was pH 5.0-11.0. The results revealed that apple and pear peels have the potential to enhance nitrification for ammonia (NH3) to nitrite (NO2) and finally to nitrate (NO3). The orange peels do not encourage the nitrification of ammonia. In addition, biomass efficiency still needs further research since 27.8g of fruit peels can only allow 2 hours of filtering the ammonia acid. The experimental results revealed that the pear peels are most suitable for filtration materials than other fruit peels. Thus, fruit peel is potentially helpful for a sustainable and eco-friendly water filtration system but still needs further improvement and development.