This paper investigates E.coli O157:H7 bacteria detection through the electrical based aluminium interdigitated electrode (Al-IDE) biosensor. Functionalization step, silanization process was done using 3-aminopropyl triethoxysilane (APTES). Immobilization process was done using carboxylic probe E.coli. The physical properties of the biosensor were characterized by using low power microscope (LPM), high power microscope (HPM), and atomic force microscopy (AFM). The I-V characteristics were performed for every functionalization step consists of bare Al-IDE, silanization, immobilization, and hybridization. Tween-20 was used as a blocking agent for the nonspecific binding on the non-immobilized area of the biosensor surface. The biosensor was validated with complementary, non-complementary, and single based mismatch ssDNA targets. Different concentrations of complementary target ssDNA from 10 fM tol μM was done for the sensitivity detection. The electrical measurements were done using picoammeter voltage source (Keithley 2450), Kickstart software, and probe station. It confirmed that Al-IDE biosensor is successfully able to detect specific nanoampere range of concentrated E.coli O157:H7 ssDNA targets.
