Home
  • English
  • Čeština
  • Deutsch
  • Español
  • Français
  • Gàidhlig
  • Latviešu
  • Magyar
  • Nederlands
  • Português
  • Português do Brasil
  • Suomi
  • Log In
    New user? Click here to register. Have you forgotten your password?
Home
  • Browse Our Collections
  • Publications
  • Researchers
  • Research Data
  • Institutions
  • Statistics
    • English
    • Čeština
    • Deutsch
    • Español
    • Français
    • Gàidhlig
    • Latviešu
    • Magyar
    • Nederlands
    • Português
    • Português do Brasil
    • Suomi
    • Log In
      New user? Click here to register. Have you forgotten your password?
  1. Home
  2. Resources
  3. UniMAP Index Publications
  4. Publications 2024
  5. Easy extraction of Ganoderma boninense liquid sample using portable on-chip device
 
Options

Easy extraction of Ganoderma boninense liquid sample using portable on-chip device

Journal
Indonesian Journal of Biotechnology
ISSN
08538654
Date Issued
2024-03-01
Author(s)
Josephin A.
Whulanza Y.
Rahman S.F.
Lischer K.
Surya M.I.
Martiansyah I.
Rahman W.
Uda Hashim
Universiti Malaysia Perlis
DOI
10.22146/ijbiotech.83645
Handle (URI)
https://hdl.handle.net/20.500.14170/5496
Abstract
Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.
Funding(s)
Badan Pengelola Dana Perkebunan Kelapa Sawit
Subjects
  • Ganoderma boninense |...

File(s)
research repository notification.pdf (4.4 MB)
google-scholar
Views
Downloads
  • About Us
  • Contact Us
  • Policies