Screening of process parameters to produce Xylanase from Aspergillus niger for secondary bioethanol production

In recent years, the biotechnological use of xylanases has grown remarkably. Xylanase is a hydrolytic enzyme with a broad industrial application. In specific, xylanase can convert xylan into xylose, a fermentable sugar source for secondary bioethanol production. The objective on this study is to investigate the significance of different parameter effects for an efficient xylanase production from Aspergillus niger (A. niger). In this study, four factors: incubation temperature, medium pH, incubation time, and agitation speed were screened by performing One-factor-at-a-time (OFAT) analysis. Xylanase production with the maximal enzyme activity was successfully obtained from OFAT analysis under condition of 32°C, pH 5.0, 5 days, and 150 rpm.