Deep eutectic solvent for extraction of natural antioxidant from a medicinal plant, Coleus aromaticus

Antioxidant is a basic requirement for maintaining good health. In this research, natural antioxidant was extracted from <jats:italic>C. aromaticus</jats:italic> by using different types of synthesized deep eutectic solvents (DES). The extracts were analysed using total phenolic content assay and DPPH radical scavenging assay. Finally, HPLC and FTIR methods were employed to check the present of target compounds. From the result obtained, the best DES was ChCl : glycerol with a ratio of 1:4 bearing 97.25 µg/ml of total phenolic content. The bioactive compounds that were successfully identified through HPLC and FTIR assays were Rosmarinic acid, Caffeic acid, Gallic acid and Quercetin. This shows DES is a potential green solvent to extract bioactive compounds present in medicinal plants.