This study aims to observe the sensing properties of chitosan sensor (CS) and chitosanroselle sensors (CRSs) on toluene at various concentrations. CRSs were deposited using various chitosan: roselle ratio of 99:1 to 89:11 to find the optimum ratio which exhibit the best electrical properties. Toluene is a suitable chemical marker for lung cancer diagnosis where 30 ppb toluene in exhaled breath is regarded to be a distinctive early symptom of lung cancer. Therefore, the preliminary study on the electrical laboratory testing of the CS and CRSs film properties to toluene vapor-contaminated air in the range of 15–90 ppb was carried out at room temperature (25–30oC). The results suggested that the proposed CS and CRS can operate at room temperature with a high performance demonstrated by good response, recovery, sensitivity, repeatability, stability and selectivity. All CRS exhibited better output voltage compared to CS where the optimum ratio observed was chitosan:roselle (93:7). This is a good preliminary step for a trouble free, painless and steadfast technique which aid in diagnosing lung cancer, enabling early detection.