Publication:
Enhanced butanol production by optimization of medium parameters using Clostridium acetobutylicum YM1

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cris.author.scopus-author-id 37107225800
cris.author.scopus-author-id 56248038900
cris.author.scopus-author-id 35108679300
cris.author.scopus-author-id 25639525900
cris.author.scopus-author-id 15019967700
cris.author.scopus-author-id 7103120115
dc.contributor.author Al-Shorgani N.
dc.contributor.author Shukor H.
dc.contributor.author Abdeshahian P.
dc.contributor.author Kalil M.
dc.contributor.author Yusoff W.
dc.contributor.author Hamid A.
dc.date.accessioned 2025-01-13T13:07:28Z
dc.date.available 2025-01-13T13:07:28Z
dc.date.issued 2018-11-01
dc.description.abstract A new isolate of the solvent-producing Clostridium acetobutylicum YM1 was used to produce butanol in batch culture fermentation. The effects of glucose concentration, butyric acid addition and C/N ratio were studied conventionally (one-factor-at-a-time). Moreover, the interactions between glucose concentration, butyric acid addition and C/N ratio were further investigated to optimize butanol production using response surface methodology (RSM). A central composite design was applied, and a polynomial regression model with a quadratic term was used to analyze the experimental data using analysis of variance (ANOVA). ANOVA revealed that the model was highly significant (p < 0.0001) and the effects of the glucose and butyric acid concentrations on butanol production were significant. The model validation experiment showed 13.82 g/L butanol was produced under optimum conditions. Scale up fermentation in optimized medium resulted in 17 g/L of butanol and 21.71 g/L of ABE. The experimental data of scale up in 5 L bioreactor and flask scale were fitted to kinetic mathematical models published in the literature to estimate the kinetic parameters of the fermentation. The models used gave the best fit for butanol production, biomass and glucose consumption for both flask scale and bioreactor scale up.
dc.identifier.doi 10.1016/j.sjbs.2016.02.017
dc.identifier.scopus 2-s2.0-85047399491
dc.identifier.uri https://hdl.handle.net/20.500.14170/13011
dc.relation.grantno UKM-DLP-2012-007
dc.relation.ispartof Saudi Journal of Biological Sciences
dc.relation.ispartofseries Saudi Journal of Biological Sciences
dc.relation.issn 1319562X
dc.rights open access
dc.subject Butanol | Clostridium acetobutylicum YM1 | Fermentation | Kinetics | Optimization | Scale-up
dc.title Enhanced butanol production by optimization of medium parameters using Clostridium acetobutylicum YM1
dc.type Journal
dspace.entity.type Publication
oaire.citation.endPage 1321
oaire.citation.issue 7
oaire.citation.startPage 1308
oaire.citation.volume 25
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
oairecerif.affiliation.orgunit Universiti Kebangsaan Malaysia
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person.identifier.scopus-author-id 37107225800
person.identifier.scopus-author-id 56248038900
person.identifier.scopus-author-id 35108679300
person.identifier.scopus-author-id 25639525900
person.identifier.scopus-author-id 15019967700
person.identifier.scopus-author-id 7103120115
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