Publication:
Effect of abiotic elicitor on the production of camptothecin from Nothapodytes nimmoniana (J. Graham) Mabb

Simple item page
cris.author.scopus-author-id 57032173200
cris.author.scopus-author-id 58111545800
cris.author.scopus-author-id 58420057900
cris.author.scopus-author-id 58592315500
cris.author.scopus-author-id 58590979600
cris.author.scopus-author-id 57664864200
cris.author.scopus-author-id 7006558013
cris.author.scopus-author-id 57219716693
cris.virtual.department Universiti Malaysia Perlis
cris.virtualsource.department 3f983c2e-28f9-4f7e-9c19-b081a6c20fd8
dc.contributor.author Krishnan N.
dc.contributor.author Iyer R.
dc.contributor.author Priyadarshini S.
dc.contributor.author Kundu A.
dc.contributor.author Natarajan P.
dc.contributor.author Singh P.K.
dc.contributor.author Subash Chandra Bose Gopinath
dc.contributor.author Raman P.
dc.date.accessioned 2024-10-04T00:44:25Z
dc.date.available 2024-10-04T00:44:25Z
dc.date.issued 2023-10-01
dc.description.abstract The plant Nothapodytes nimmoniana belongs to the Icacinaceae family, and is a significant source of camptothecin (CPT), an effective quinoline alkaloid with an array of pharmacological benefits including anti-cancer, anti-HIV, antibacterial, and antioxidant etc. The elicitation method is the most effective strategy to increase and maintain the synthesis of particular compounds in vitro culture. In this study, to understand the impact of various abiotic elicitors on CPT production by using established N. nimmoniana cell suspension culture obtained from friable leaf callus grown in Murashige & Skoog basal media with sucrose and agar augmented with 2 mg/L of 2,4-dichloro phenoxy acetic acid (2,4-D). A total of six abiotic elicitors were used such as cadmium chloride, ethanol, salicylic acid, tryptophan, copper chloride, and calcium chloride. The impact of these abiotic elicitors on the CPT yield at three different concentrations by cell suspension was evaluated for up to 15 days. High-performance liquid chromatography (HPLC) was used to quantify the CPT production. A maximum of 9.43 and 6.26-fold increment was recorded in CPT production in suspension culture elicitated with 2 mM of tryptophan and 1 mM of tryptophan on the 12th day of inoculation followed by ethanol 7.5% treated cell suspension which enhanced the CPT production up to 1.44-fold increase while compared to control. The cell viability of the suspension culture treated with calcium chloride and cadmium chloride was observed in decreased biomass as per the increment in the concentration. The findings of the current study indicate the potential use of abiotic elicitation as another possible option for boosting up the synthesis of CPT in N. nimmoniana cell suspension cultures.
dc.identifier.doi 10.1016/j.bcab.2023.102846
dc.identifier.scopus 2-s2.0-85171474065
dc.identifier.uri https://hdl.handle.net/20.500.14170/9265
dc.language.iso en
dc.relation.funding SRM Institute of Science and Technology
dc.relation.grantno SRMU-HS-BT10
dc.relation.ispartof Biocatalysis and Agricultural Biotechnology
dc.relation.ispartofseries Biocatalysis and Agricultural Biotechnology
dc.relation.issn 18788181
dc.subject Abiotic elicitor | Camptothecin | Cell suspension | Elicitation | Nothapodytes nimmoniana
dc.title Effect of abiotic elicitor on the production of camptothecin from Nothapodytes nimmoniana (J. Graham) Mabb
dc.type Journal
dspace.entity.type Publication
oaire.citation.volume 53
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit SRM Institute of Science and Technology
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.citation.number 102846
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid 0000-0002-8347-4687
person.identifier.orcid 0000-0003-2739-4882
person.identifier.scopus-author-id 57032173200
person.identifier.scopus-author-id 58111545800
person.identifier.scopus-author-id 58420057900
person.identifier.scopus-author-id 58592315500
person.identifier.scopus-author-id 58590979600
person.identifier.scopus-author-id 57664864200
person.identifier.scopus-author-id 7006558013
person.identifier.scopus-author-id 57219716693
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Research repository notification.pdf
Size:
4.4 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Publications 2023