As know Polylactic acid (PLA) is a popular and widely used thermoplastic material used in fused filament fabrication (FDM) due to its biodegradability and biocompatibility. The aim of this paper is to study the effect of extrusion parameters on the quality of the 3D printing filament. The study began by extruding of 3D850D grade PLA pellet from Ingeo Natureworks into a single filament extruder machine with an extrusion temperature range of 165 °C to 185 °C to and a screw rate of 2rpm to 6rpm. The 90° raster angle of line infill pattern with 100% infill density were used for the 3D printing specimens. Taguchi method was used to design the experiment. Mechanical test standards such as ASTM D638, ASTMD695 and ASTM D790 and was used for tensile, compression and flexural testing respectively to analyze the quality of the extruded PLA filament. According to the result obtained, the optimum parameter for highest mechanical strength was obtained at an extrusion temperature of 175 °C to 180°C and screw speed of 4-5 rpm as the filament extruded meets the required diameter of commercial 3D printing filament.