In this work, we exploit template-free electrodeposition based on chronocoulometry to deposit a homogeneous nanostructured Pt film on a miniaturized Pt electrode. The results demonstrate that the introduction of a nanostructured surface remarkably improves the performance of miniaturized Pt electrode as a glucose sensor. The nanostructured Pt electrode produces larger current response than that of the bare Pt electrode with an identical apparent area. It also had a higher current response and a wider linear range owing to the presence of larger surface area.