In 1977, there are two scientists, first used the rRNA sequence to study the overall phylogenetic structure of living world, who are Carl Woese and George E. Fox. Herein, based on their foundation, we prepared a set of broad range of 16s rRNA to compare the differences and select the common non-conserved region of the sequence to be used as a probe. We availed complete gene sequences from different species of pathogenic bacteria through the National Center for Biotechnology Information, then align the sequence and performed the comparison and analysis. The identified sequences can be used for the potential biosensing applications.
