On the basis of the in situ analysis of the sturgeon condition within the Lower Danube River, the aim was to carry out a research that would complement the results obtained from the monitoring and ultrasonic tagging methods. Thus, DNA samples were taken selectively from each ultrasonically tagged specimen in order to determine whether or not there is a possibility of hybridization between the species Acipenser stellatus and Huso huso. Thus, the microsatellite loci were investigated and certain parameters like diagnostic alleles, repeated sequences and specific molecular markers which were used to determine the degree of hybridism.