The aim of this work was to study the effectiveness of pretreatment methods by developing a comparative study of diluted acetic acid and hydrothermal pretreatment on reducing sugar production and inhibitors formation (Furfural) from the mixed rice biomass. Diluted acetic acid and hydrothermal pretreatment were carried out over a range of temperature (150 – 190 ºC) within 30 minutes to observe the reducing sugar production and inhibitor formation (Furfural). Seamen Model was used to obtain the rate of reaction in depolymerisation of lignocellulosic biomass. This experiment data were fitted the first-order kinetic model to obtain the reaction rate constant of production and degradation of sugar. In addition, Arrhenius equation was used to determine the activation energy of the reaction. From the result, it showed that the diluted acetic acid pretreatment had higher rate on reducing sugar production and inhibitor formation than the hydrothermal pretreatment. The activation energy for the formation sugar in hydrothermal pretreatment is found 29.1 kJ/mol which is higher than acetic acid pretreatment (23.9 kJ/mol).
