The aim of this work was to produce glucose from sugarcane waste. It consists of high cellulose, hemicellulose and lignin which enables to be converted into a glucose production. Three conditions of acid hydrolysis was measured by using One Factor at a Time (OFAT) which consist of sulphuric acid concentration, retention time and temperature. The highest yield and optimum conditions from OFAT parameters were further continue with the enzymatic hydrolysis by using Central Composite Design (CCD). The optimization of enzymatic hydrolysis for the conversion of cellulose to glucose was conducted by using three independent variables which were pH, temperature and enzyme dosage. Based on the data of Design of Expert (DOE) software from optimization of enzymatic hydrolysis, the optimum glucose production was determined by using Dinitrosalicylic Acid Reagent (DNS) method.
