Publication:
Growth rate and morphology studies of grey oyster mushroom (Pleurotus Pulmonarius) using Chlamydospore induction liquid spawn
Growth rate and morphology studies of grey oyster mushroom (Pleurotus Pulmonarius) using Chlamydospore induction liquid spawn
Date
2021
Authors
Chong Shi Fern
Journal Title
Journal ISSN
Volume Title
Publisher
Research Projects
Organizational Units
Journal Issue
Abstract
Spawn preparation from chlamydospore which is produced through spore induction technique has become a potential and promising technique in grey oyster mushroom cultivation. How long that chlamydospore can survive in the induction media and ability to grow in a large scale production had raised questions and need to be studied. Hence, in this study, spore induction technique was used to induce chlamydospore from grey oyster mushroom and the effect of storage duration on chlamydospore growth rate in flask and bioreactor were investigated. It was found that chlamydospores inoculum stored at 4°C and ambience condition for 8 months was still able to grow mycelium with average mycelial dry weight at 0.3100g and 0.3075g respectively. Mycelium inoculum however able to store up to 4 months with average mycelial dry weight at 0.2555g only. Chlamydospores inocula stored for 1 to 7 months took 33 days for colonization completion and increased to 36 days in the next month and so on. The highest growth rate obtained was from 2month’s stored chlamydospore inoculum which was 0.9105cm day-1 compared to the highest growth rate obtained from mycelium inoculum which was only 0.8661cm day-1 only. For the total yield production, 2 month’s store chlamydospore inoculum had also observed the highest recorded as 431.00g. The highest specific growth rate was obtained from 5 month’s stored chlamydospore inoculum at 0.0088g hour-1 with shortest doubling time 78.75 hours when cultivated in bioreactor. While the slowest was obtained from 5 month’s stored mycelium inoculum with specific growth rate at 0.0069g hour-1 and longest doubling time of 100.43 hours. The sustainability of active mycelium biomass was verified with the analysis of glucose concentration in the media where sugar uptake was high for chlamydospore at 1 month’s and 5 month’s stored chlamydospore. For the three months cultivation period, a total of 430.74g and 422.35g were obtained for 1 month’s and 5 month’s storage period of chlamydospore respectively. This study proved that chlamydospore from grey oyster mushroom is a potential inoculum for long term storage and revival ability on large scale production
Description
Master of Science in Bioprocess Engineering
Keywords
Grey Oyster Mushroom (Pleurotus pulmonarius),
Mushroom,
Chlamydospore induction liquid spawn,
Mycelium inoculum