The aim of this study is to analysis the sound transmission loss (STL) for the laminated glass and Perspex. In this study, the STL of laminated glass and Perspex are determined using the two-load transfer function method (TFM) impedance tube measurement setup. There are 5 mm and 8 mm thickness of glass and Perspex were used. Glass and Perspex will be laminated into double and triple layer in order to investigate its STL due to interlayer effect. All the specimens are prepared into round shape with the diameter of 33 mm that fit with the inner diameter of impedance tube. From the measurement, it is concluded that the STL almost similar for the Perspex and glass without the layer lamination at the frequency range below 3500Hz. Number of interlayer for the glass or Perspex lamination plays the major role as main parameters that affect the overall STL.