Publication:
Red Spectral Shift in Sensitive Colorimetric Detection of Tuberculosis by ESAT-6 Antigen-Antibody Complex: a New Strategy with Gold Nanoparticle

Simple item page
cris.author.scopus-author-id 57226172765
cris.author.scopus-author-id 55654521800
cris.author.scopus-author-id 7006558013
dc.contributor.author Wang F.a.
dc.contributor.author Lakshmipriya T.
dc.contributor.author Gopinath S.C.B.
dc.date.accessioned 2025-01-13T14:46:00Z
dc.date.available 2025-01-13T14:46:00Z
dc.date.issued 2018-01-01
dc.description.abstract Tuberculosis (TB) is a highly contagious life-threatening disease caused by the bacterial pathogen Mycobacterium tuberculosis. ESAT-6, an abundant early secretory antigenic target protein by M. tuberculosis, found to play a vital role in virulence. Developing a friendly method for the detection of ESAT-6 at the lower concentration facilitates to treat TB at an earlier stage and helps to control the spreading of disease. Herein, a new single-step approach was designed and was done by pre-mixing ESAT-6 and antibody before being added to the gold nanoparticle (GNP) followed by the salt-induced aggregation. We could attain the detection limit of 1.25 pM, showing the aggregation of GNP and the red spectral shift. Further, a higher specificity was demonstrated with the lack of electrostatic biofouling by ESAT-6 on GNP and retained the dispersed GNP in the presence of 10-kDa culture filtrate protein from M. tuberculosis. The required precise antibody concentration for this assay was found to be 60 nM. The increment in the antibody concentration from 75 nM drastically diminishes the sensitivity to ~ 680-fold, due to the crowding effect. With this assay, we attested the suitability of colorimetric assay for efficiently detecting the smaller-sized protein.
dc.identifier.doi 10.1186/s11671-018-2753-5
dc.identifier.scopus 2-s2.0-85055318772
dc.identifier.uri https://hdl.handle.net/20.500.14170/13414
dc.relation.grantno undefined
dc.relation.ispartof Nanoscale Research Letters
dc.relation.ispartofseries Nanoscale Research Letters
dc.relation.issn 19317573
dc.rights open access
dc.subject Antibody | Colorimetric assay | ESAT-6 antigen | Gold nanoparticle | Tuberculosis
dc.title Red Spectral Shift in Sensitive Colorimetric Detection of Tuberculosis by ESAT-6 Antigen-Antibody Complex: a New Strategy with Gold Nanoparticle
dc.type Journal
dspace.entity.type Publication
oaire.citation.volume 13
oairecerif.affiliation.orgunit Pingdingshan University
oairecerif.affiliation.orgunit Universiti Teknologi PETRONAS
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.citation.number 331
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid #PLACEHOLDER_PARENT_METADATA_VALUE#
person.identifier.orcid 0000-0002-8347-4687
person.identifier.scopus-author-id 57226172765
person.identifier.scopus-author-id 55654521800
person.identifier.scopus-author-id 7006558013
Files
Collections
Publications 2018