Tijjani Adam
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Preferred name
Tijjani Adam
Official Name
Tijjani, Adam
Alternative Name
Adam, T.
Adama, Tijjani
Adam, Tijjani
Adam, Tijjan
Tijjani, A.
Main Affiliation
Scopus Author ID
55074964600
Researcher ID
AAH-5534-2019

Research Output
Now showing 1 - 3 of 3
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Publication

Selective detection of alpha synuclein amyloid fibrils by faradaic and non-faradaic electrochemical impedance spectroscopic approaches

2025-02 , Hussaini Adam , Subash Chandra Bose Gopinath , Hemavathi Krishnan , Tijjani Adam , Makram A. Fakhri , Evan T. Salim , A. Shamsher , Sreeramanan Subramaniam , Yeng Chen

This study utilized faradaic and non-faradaic electrochemical impedance spectroscopy to detect alpha synuclein amyloid fibrils on gold interdigitated tetraelectrodes (AuIDTE), providing valuable insights into electrochemical reactions for clinical use. AuIDE was purchased, modified with zinc oxide for increased hydrophobicity. Functionalization was conducted with hexacyanidoferrate and carbonyldiimidazole. Faradaic electrochemical impedance spectroscopy has been extensively explored in clinical diagnostics and biomedical research, providing information on the performance and stability of electrochemical biosensors. This understanding can help develop more sensitive, selective, and reliable biosensing platforms for the detection of clinically relevant analytes like biomarkers, proteins, and nucleic acids. Non-faradaic electrochemical impedance spectroscopy measures the interfacial capacitance at the electrode–electrolyte interface, eliminating the need for redox-active species and simplifying experimental setups. It has practical implications in clinical settings, like real-time detection and monitoring of biomolecules and biomarkers by tracking changes in interfacial capacitance. The limit of detection (LOD) for normal alpha synuclein in faradaic mode is 2.39-fM, The LOD for aggregated alpha synuclein detection is 1.82-fM. The LOD for non-faradaic detection of normal alpha synuclein is 2.22-fM, and the LOD for nonfaradaic detection of aggregated alpha synuclein is 2.40-fM. The proposed EIS-based AuIDTEs sensor detects alpha synuclein amyloid fibrils and it is highly sensitive.

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Publication

Aluminium interdigitated electrode with 5.0 μm gap for electrolytic scooting

2024-06 , Uda Hashim , Muhammad Nur Aiman Uda , Tijjani Adam , Asral Bahari Jambek , Ismail Saad , Nor Azizah Parmin , Shahidah Arina Shamsuddin , Nursakinah Abdul Karim , G. Yashni , Nur Hulwani Ibrahim , N. Parimon , M. F. H. Rani

The goal of the research project is to design, fabricate, and characterize an extremely sensitive biosensor for use in healthcare. Using AutoCAD software, a novel IDE pattern with a 5 μm finger gap was created. Conventional photolithography and regular CMOS technology were used in the fabrication process. A 3D nano profiler, scanning electron microscopy (SEM), high-power microscopy (HPM), and low-power microscopy (LPM) were used to physically characterize the manufactured IDE. Chemical testing was done using several pH buffer solutions, and electrical validation was performed using I-V measurements. The Al IDE was produced, with a tolerance of 0.1 μm between the fabricated IDEs and the design mask. Electrical measurements verified the flawless fabrication of the IDE, and the device's repeatability was validated by the outcomes of comparable IDE samples. For each pH buffer solution, a modest additional volume of 2 μl was used to quantitatively detect slight current fluctuations in the microampere range. Through pH calibration for advanced applications in the realm of chemical sensors using an amperometric method, this research study has verified the chemical behavior of the IDE.

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Advancing COVID-19 detection high-performance RNA biosensing via electrical interactions

2024-06 , Uda Hashim , Muhammad Nur Afnan Uda , Muhammad Nur Aiman Uda , Tijjani Adam , Nur Hulwani Ibrahim , Chai Chang Yii , Lorita Angeline

This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process was facilitated by the RNA Probe specific to COVID-19. To verify its specificity in detection, the functionalized biosensor was tested against single-base mismatches, non-complementary sequences, and complementary sequences. The physical characteristics of the Al-IDE biosensor were examined using both low-power microscopy (LPM) and high-power microscopy (HPM). Additionally, the morphological properties of the biosensor were assessed using atomic force microscopy (AFM). To assess its diagnostic potential, the biosensor's sensitivity was evaluated by exposing it to a range of complementary targets, spanning from 1 femtomolar (fM) to 1 micromolar (μM). The current-voltage (I-V) characteristics of the biosensor were meticulously analyzed at each stage of functionalization bare Al-IDE, silanization, immobilization, and hybridization. This I-V characterization was carried out using a picoammeter voltage source (Keithley 2450), Kickstart software, and a probe station. The results confirmed the biosensor's capability to effectively detect COVID-19 targets within the nanoampere concentration range, demonstrating its success in detecting specific COVID-19 targets at the nanoampere level.

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