Siti Nur Aishah Mat Yusuf
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Siti Nur Aishah Mat Yusuf
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Siti Nur Aishah, Mat Yusuf
Alternative Name
Yusuf, Siti Nur Aishah Mat
Mat Yusuf, Siti Nur Aishah
Mat Yusuf, S.N.A.
Yusuf, S.N.A.M.
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57193059379

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  • Publication
    Antiproliferative Properties of the Synthesised Mariposa Christia vespertilionis Silver Nanoparticles
    ( 2023-06-09)
    Siti Nur Aishah Mat Yusuf
    ;
    Chan J.Y.
    ;
    Zaini T.A.
    ;
    Chiu H.I.
    Mariposa Christia vespertilionis (MCV) has gained attention in cancer medicine development. Nowadays, plant-based nanoparticles have been widely used in drug delivery as they bring minimal effects to healthy cells and exhibiting antiproliferative effects to cancer cells. This study was conducted to investigate the antiproliferative properties of synthesised silver nanoparticles (AgNPs) using MCV aqueous leaf extract on HT29 cell line. The qualitative phytochemical analysis has proved that flavonoids, alkaloids, glycosides and tannins were present in MCV. Based on the ultraviolet-visible (UV-vis) spectrometry spectrum, the optimum conditions to synthesise highest amount of AgNPs were 15% v/v of extract concentration and 24 h of incubation period. The synthesised AgNPs were characterised by Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analysis. Flavonoids, alkaloids, glycosides and tannins were responsible in AgNPs formation based on FTIR analysis. In TEM analysis, the obtained nanoparticles were well-distributed with spherical shape. The particle size was in the range from 30 nm to 50 nm. From dimethylthiazoldiphenyltetrazolium bromide (MTT) assay, the synthesised AgNPs was proven to have ability in killing cancer cells (IC50: <12.5 µg/ml). They showed higher killing effect compared to the extract alone but lower than chemotherapy drug 5-fluorouracil (5FU). In conclusion, this study proved that MCV and synthesised AgNPs have the ability to kill cancer cells.
  • Publication
    Phyto-synthesis of Silver Nanoparticles from Mariposa Christia vespertilionis and Its Antioxidative Properties
    ( 2023-06-09)
    Siti Nur Aishah Mat Yusuf
    ;
    Arif N.A.M.
    ;
    Sukri M.H.H.
    ;
    Noor N.S.
    Mariposa Christia vespertilionis (MCV) is also known as butterfly wing plant, or commonly known as ‘pokok rerama’ in Malaysia. This plant belongs to the Fabaceae family. Its bioactive compounds including triterpenes, alkaloids, fatty acids, phenol, alkanes and alcohol have shown antioxidant activity. The objective of this study is to synthesis silver nanoparticles together with MCV leaves extract using ultrasonic-assisted extraction (UAE) as an extraction method and to evaluate its antioxidative activity. The best conditions (concentration of silver nitrate and incubation temperature) for formation of AgNPs were visualised by UV-Vis spectroscopy. The absorbance peaks for both conditions were 450 nm and 430 nm respectively. Both MCV extract and synthesized nanoparticles have been characterized by Fourier-transform infrared spectroscopy (FTIR) to determine the functional group that responsible in reduction of silver ions. The AgNPs was also characterized by using Transmission Electron Microscopy (TEM) to determine the morphology and size of the synthesised silver nanoparticles (30 to 50 nm). Total phenolic content and total flavonoids content were also conducted for MCV extracts thus the value achieved was 0.429 ± 0.046 mg GAE/g and 0.033 ± 0.033 mg QU/g. Antioxidant activity was done by using DPPH solution. The scavenging activity for MCV extract was higher compared to the synthesised AgNPs. As conclusion, MCV leaves has potential as antioxidant agent which may be beneficial in preventing degenerative diseases.