Muhammad Nur Aiman Uda
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Preferred name
Muhammad Nur Aiman Uda
Official Name
Muhammad Nur Aiman, Uda
Alternative Name
Uda, M. N.A.
Aiman Uda, Muhammad Nur
Uda, M. N.A.
Main Affiliation
Scopus Author ID
55984680000
Researcher ID
V-7751-2019

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Production and characterization of graphene from carbonaceous rice straw by cost-effect extraction

2021-05-01 , Muhammad Nur Aiman Uda , Subash Chandra Bose Gopinath , Uda Hashim , Halim N.H. , Nor Azizah Parmin , Muhammad Nur Afnan Uda , Anbu P.

This paper describes the synthesis of graphene-based activated carbon from carbonaceous rice straw fly ash in an electrical furnace and the subsequent potassium hydroxide extraction. The produced graphene has a proper morphological structure; flakes and a rough surface can be observed. The average size of the graphene was defined as up to 2000 nm and clarification was provided by high-resolution microscopes (FESEM and FETEM). Crystallinity was confirmed by surface area electron diffraction. The chemical bonding from the graphene was clearly observed, with –C=C– and O–H stretching at peaks of 1644 cm−1 and 3435 cm−1, respectively. Impurities in the graphene were found using X-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. The measured size, according to zeta-potential analysis, was 8722.2 ± 25 nm, and the average polydispersity index was 0.576. The stability of the mass reduction was analyzed by a thermogravimetric at 100 Â°C, with a final reduction of ~ 11%.

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Design 5.0 µm Gap Aluminium Interdigitated Electrode for Sensitive pH Detection

2020-07-09 , Muhammad Nur Afnan Uda , Asral Bahari Jambek , Uda Hashim , Muhammad Nur Aiman Uda , Nor Azizah Parmin , Bakar A.H.A. , Anuar A. , Bakar M.A.A. , Sulaiman M.K.

The aim of the research study to design high sensitive biosensor for medical applications. IDE pattern was designed using AutoCAD software with 5 µm ginger gap. The fabrication process was done using a conventional photolithography process and standard CMOS process. The fabricated electrode was physically characterized using a low power microscope (LPM) and a high power microscope (HPM). The electrically validated through I-V measurements and chemically tested with different pH buffer solutions. Al IDE was well fabricated with 0.1 µm tolerance between the design mask and fabricated IDEs. Electrical measurements confirmed that IDE was well fabricated without any shortage and results of similar IDE samples were confirmed that the repeatability of the device. The extremely small current variations in nano ampere range were quantitatively detected using an extra small volume of 2 µl for different pH buffer solutions. It is confirmed that IDEs are sensitive in both alkali and hydroxyl ions medium.

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New Development Quantification Methods for Salt Iodine and Urinary Iodine Using Microfluidics Based Nanotechnology

2020-03-18 , Nur Hulwani Ibrahim , Midhat Nabil Ahmad Salimi , Muhammad Nur Aiman Uda , Nor Azizah Parmin , Uda Hashim , Muhammad Nur Afnan Uda

In Malaysia, the first Iodine Deficiency Disorders (IDD) survey was conducted in 1996 and it was discovered that Peninsular Malaysia did not have IDD problem until latter studies showed goitre occurrence of 34.7% in Hulu Langat district and urinary iodine lower than the adequate level of (100-199 ug/L) in Perak and Pahang states (Selamat et al., 2010). Baseline and periodical sampling of children and pregnant woman urine and imported salt commodities for the consumption of the population is mandatory for iodine measurement. Thus, development of quantitative methods of measurement of salt and food iodine is crucial for implementation of the USI program nationwide. In this study, interdigitated electrode (IDE) biosensor, a rapid, sensitive and selective method has been developed to determine the iodine content in both urine and salt. This method includes functionalization and silanization step using 3-aminopropyl triethoxysilane (APTES). The I-V characterization of IDE biosensor was performed using (Keithley 2450), Kickstart software and Probestation. It measures the amount of current flow through IDE which is directly proportional to the concentration of iodine in both urine and salt. Hence, IDE biosensor is proven to be a rapid, selective, sensitive method and can be developed as a new nanotechnology for the elimination of Iodine Deficiency Disorders (IDD) among children and pregnant woman.

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Nano-micro-mili Current to Mili Voltage Amplifier for Amperometric Electrical Biosensors

2020-03-18 , Muhammad Nur Afnan Uda , Parmin N.A. , Asral Bahari Jambek , Uda Hashim , Muhammad Nur Aiman Uda , Shaharuddin S.N.A. , Adam H.

Amplification of nano and mircoampere electrical signal to the detectable range is essential in the biosensor field. This research is mainly focused on design an amplifier circuit to capture and amplify three different range of current as nano, micro and mili ampere and convert it to detectable voltage range as an output signal to the processing circuit. The Proteus 8 Pro software was used to design, simulate and calibrate the amplifier circuit. Firstly, current input as mili, micro and nano current were flown through 0.1 m, 10 and 10 K resistors, respectively to convert different current inputs to the similar range in micro voltage. The MAX 4238 opamp IC was used to amplify micro voltage to mili voltage. LM 358 dual operational amplifier was used to supply virtual ground to MAX 4238 amplifier. The amplified output voltage of three different current inputs as nano, micro and mili were nearly equal to theoretical outputs.

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Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection

2020-07-09 , Mhd Akhir M.A. , Nor Azizah Parmin , Uda Hashim , Subash Chandra Bose Gopinath , Rejali Z. , Afzan A. , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Voon Chun Hong

This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there was no HPV strain 18 detected in the DNA from saliva samples.

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Evaluation and Optimization of Genomic DNA Extraction from Food Sample for Microfluidic Purpose

2020-03-18 , Muhammad Nur Afnan Uda , Nor Azizah Parmin , Asral Bahari Jambek , Uda Hashim , Muhammad Nur Aiman Uda , Shaharuddin S.N.A.

Contamination of various food samples became one of the critical issues in food pathogen infection. Food pathogen can be detected by using digital polymerase chain reaction (PCR) and sequencing. These methods were reliable but consuming and take a longer time for detection. The present work describes the innovation to develop a technology to extract double-stranded deoxyribonucleic acid (dsDNA) from food samples and then denatured dsDNA into and single-strand DNA (ssDNA) for further use on the chip using microfluidic device. Microfluidic device is a lab-on-chip device that consist of microfluidic channels that provide paths for biomolecules to flow to individual point of care. DNA extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. Lysis solution is used in the process of extraction the DNA to break up the cells containing DNA from protein and other cellular materials. This extraction firstly be done in the most labour-intensive in obtaining the DNA biomolecules. Extraction methods may require an overnight incubation, may be a protocol that can be completed in minutes or a couple of hours by using a commercial kit. The disadvantages of the laboratory and commercial kit is due to time-consuming, poor cost-effectiveness, the need to use big laboratory and a complicated process which need an expertise to conduct the experiment and interpret the data. This research is proposed to design and fabricate a microfluidic device that has DNA extraction capabilities. In this research DNA extraction using a commercial kit will be used as a comparison for the quality of the result. The microfluidic device can be used in health care delivery system and will help the doctors in diagnostic process to identify disease of a patient rapidly. Other than that, the output extracted from microfluidic device will be used for DNA probe target interaction for diagnostic kit. The major advantage of microfluidic device is that it consumes less time compared to the conventional chemical methods.

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Quantitative lead (Pb+) ion detection via modified silicon nanowire

2021-05-03 , Uda Hashim , Tijjani Adam , Nuri A KH Ehfaed , Muhammad Nur Afnan Uda , Muhammad Nur Aiman Uda

The study presented a functionalized silicon with (3-aminopropyl) triethoxysilane (APTES) to serve as a sensor for heavy metal detection. The amino-functionalized Si nanowires were tested against the heavy metal, lead (Pb). Due to the silicon electrochemical response towards the heavy metal ions, linear response to four different sources of water was observed. The results indicated that Pb can be detected with a high precision. Furthermore, confirmation was demonstrated using atomic absorption spectroscopy to determine the level lead content in the collected water source. Tab water (H2O), River H2O, Treated (H2O), DI (deionized) H2O and found the levels of 0.0859 mg/L, 0.0929 mg/L, 0.0052mg/L, 0.0023 mg/L with 5.8pA, 7.2pA, 4.6pA, 3.3pA current responses, respectively. Thus, with this high capability to discriminate water samples, the sensor potential can be employed for effective heavy metal detections and further be extended to a large sensor network in water treatment plant. A new microfluidic bonding material based on SU8 to implement electrical Nano chemical sensors for ions sensing, and an investigation of direct electrical measurement allowed for label free detection.. Thus, the study developed new room temperature bonding method using SU8 as an intermediate adhesive layer. The SU8 modified bonding was compared with non modified. The bond strength of SU8 modified was found to be stronger than ordinary plasma bonding under the same curing conditions. Overnight room temperature curing yields an average burst pressure of 420 kPa, which is more than adequate for many PDMS sensor devices. In contrast, non SU8 coated plasma bonded resulted in a burst pressure of only 174 KPa.

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Distinct Detection of Ganoderma Boninense On Metal Oxides-Gold Nanoparticle Composite Deposited Interdigitated Electrode DNA sensor

2021-12-14 , Thivina V. , Uda Hashim , Subash Chandra Bose Gopinath , Adilah Ayoib , Nordin N.K.S. , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda

Oil palms suffer severe losses due to Ganoderma boninense infection that causes Basal Stem Rot (BSR). The available detection measuring the severity of BSR disease have not proved satisfactory output. Due to the influence of oil palm industry in country's economy, effective and efficient means of diagnostic measure is mandatory. Among the available diagnostic tools, biosensors were redeemed to yield the most rapid and selective results. To overcome the current issues, herein Interdigitated Electrode (IDE) electrochemical DNA biosensor to detect Ganoderma boninense was successfully designed and fabricated by thermal deposition. Lift-off photolithography fabrication process was applied followed by the surface chemical functionalization via seed deposition. Zinc Oxide (ZnO) and Titanium Dioxide (TiO2) were overlaid and the functionalized metal oxides IDE surfaces were used to detect DNA sequence complementation from Ganoderma boninense. Furthermore, gold nanoparticles were doped to increase the surface to volume ratio and enhance biocompatibility. Characterizations were made by validating the sensor's topology characteristics and electrical characteristics. From the results recorded, it has been justified that IDE with ZnO doped with gold nanoparticles surface serves as an excellent DNA sensor for the detection of Ganoderma boninense with a remarkable current of 290 nA and 176 nA for immobilization and hybridization respectively.

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Electrochemical DNA Biosensor based on 30 nM Gold Nanoparticle Modified Electrode by Electro Less Deposition for Human Papillomavirus (HPV) 18 E6 Region

2020-07-09 , Koo Siew Kim N.S. , Parmin N.A. , Uda Hashim , Subash Chandra Bose Gopinath , Rejali Z. , Afzan A. , Muhammad Nur Aiman Uda , Muhammad Nur Afnan Uda , Voon Chun Hong

The aim of this work was to develop a novel, simple, inexpensive, sensitive an electrochemical DNA biosensor based on interdigitated electrodes (IDEs) integrated gold nanoparticle modified electrode by electro less deposition for HPV 18. The biosensor was designed with a 30 mer E6 region of HPV 18 DNA modified probe. The E6 region has been used for their clinical importance properties and suitable as recognition biomarker region. Three different target types were tested which complementary target, non-complementary target and mismatch target. All target were analyzed for detection of HPV 18 in early stages by using Dielectric Analyzer (DA), Alpha-A High-performance Frequency Analyzer, Novocontrol Technologies, Handsagen, Germany associated with the software package Windeta. Complementary target gives a positive result in HPV detection, while non-complementary and mismatch target give negative results. IDE device with 5 nm gap sizes has demonstrated a high performance towards the detection of HPV18 ssDNA target by modified with 30 nm gold nanoparticle. The electrochemical biosensor showed better performance compared to agarose gel electrophoresis assay. This technology can be used as a new and attractive sensor development for detection of virus infection in human bodies.

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Morphological Analysis of Fabricated 5.0 μM Interdigitated Electrode (IDE)

2021-12-14 , Muhammad Nur Aiman Uda , Subash Chandra Bose Gopinath , Uda Hashim , Muhammad Nur Afnan Uda , Nor Azizah Parmin , Halim N.H. , Hashim M.K.R. , Anbu P.

The aim of this research is to study the morphological analysis of fabricated Interdigitated Electrode (IDE). This device electrode was physically characterized using 3D nano profiler, scanning electrode microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Atomic Force Microscope (AFM). Based on this analysis, IDE pattern was analyzed thoroughly based on the IDE pattern specifications with 5 μM finger gap and this research significantly will stand as a platform quantify the biomolecules in further analysis.

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