Now showing 1 - 2 of 2
  • Publication
    Optimization of Rice Bran Protein Extraction Using Choline Chloride-Glycerol Deep Eutectic Solvent Using Response Surface Methodology (RSM)
    ( 2023-01-01)
    Kamal Ramlee K.A.F.
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    Muhammad Nor I.N.
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    ;
    Mohd Zainudin M.A.
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    ;
    Nawawi M.A.
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    A by-product of the rice milling process, rice bran contains four different types of protein: albumin, globulin, prolamin, and glutelin. These proteins are widely known for being hypoallergenic and having nutritional benefits. In order to increase the value of rice bran, the protein was extracted via deep eutectic solvent (DES). Response Surface Methodology (RSM) was used to assess the impact of three operational conditions, including temperature, extraction duration, and the ratio of rice bran to DES, on the yield of rice bran protein after the precipitates were converted into powder form by freeze drying. At optimal working conditions, which were 60 Â°C, 2 h, and a 1:9 ratio of rice bran to DES, the method’s results showed that the highest extracted protein of rice bran was 16.254%. Several techniques, including Fourier transform infrared spectroscopy (FTIR) and Kjeldahl studies, have been used to demonstrate the presence of protein in rice bran powder. In the rice bran protein (RBP), amine (1640.70 cm−1), alcohol (3229.74 cm−1), and alkane (2925.37 cm−1) were all detected using FTIR analysis. Furthermore, Kjeldahl analysis revealed that 15.61% of the rice bran powder’s protein content is present. In conclusion, rice bran’s value as a functional meal can be increased by adding protein through the use of a deep eutectic solvent called green solvent.
      3  39
  • Publication
    Quantitative analysis method for zingiber officinale water extract using high-performance liquid chromatography
    ( 2024-01-01) ;
    Nik Daud N.M.A.
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    Mohd Zainudin M.A.
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    Ibrahim L.H.
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    ;
    Idham Z.
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    Anuar A.
    Quantitative analysis of the Zingiber Officinale sample using subcritical water extraction (SWE) was developed employing High-Performance Liquid Chromatography (HPLC) to bolster the advancement of this innovative green extraction process. This research focuses on three principal ginger bioactive compounds: 6-gingerol, 6-shagoal, and 10-gingerol. Various stages were undertaken to establish the quantitative analysis method, encompassing the optimisation of HPLC operating conditions and the formulation of standard calibration curves, yielding individual compound equations. A robust correlation within the calibration curve was achieved, exhibiting an r2 value of 0.9814 and an RSD of 5.00%. A simultaneous, swift, and dependable method was established with an injection time of 20 minutes and an 8-minute delay between injections, in contrast to the previous HPLC analysis requiring a 45-minute injection time for detecting and quantifying all components. Notably, no post-treatment was applied after the SWE process. This advancement allows for potential future online measurement of Zingiber Officinale bioactive compounds extracted using subcritical water extraction through this technology.
      28  7