Mohd Hishamuddin Che Mat
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Preferred name
Mohd Hishamuddin Che Mat
Official Name
Mohd Hishamuddin , Che Mat
Alternative Name
Che Mat, M. H.
Mat, M. H.C.
Mat, M. H.
Che Mat, M. H.
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Scopus Author ID
57203881674
Researcher ID
DEO-8852-2022

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Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study

2024-01-01 , Ahmad Anas Nagoor Gunny , Prammakumar N.K. , Ahmad A.A. , Subash Chandra Bose Gopinath , Bakar A.R.A. , Musa H. , Mohd Hishamuddin Che Mat

Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are considered as alternative natural sources of treatment for diabetes. Ultrasonic assisted extraction is a novel approach for extraction of phytoconstituents which gives high extraction yield of bioactive compounds. However, there has been no published information presently on the use of ethanol ultrasonic assisted extraction method for assessment of antioxidant and antidiabetic activities of M. calabura leaves. Hence, the current study aims to evaluate the in vitro antioxidant and antidiabetic activities of M. calabura leave extract. IC50 analysis was done to determine theinhibitory concentration and the results obtained from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed IC50 of gallic acid to be 1.0 µg/ml, which is lower than M. calabura leaves extract at 2.54 µg/ml, indicating that only small concentration of gallic acid was required to inhibit the free radicals at 50 %. However, IC50 analysis for amylase inhibition showed that M. calabura extract had 44.39 µg/ml antidiabetic activity compared to acarbose with 57.1 µg/ml activity. This indicates that M. calabura leaves extract has a better inhibition on amylase activity compared to the acarbose which is a synthetic drug. Further still, in silico study was carried out and the molecular docking result of eight ligands against amylase indicates quercetin had the least binding free energy of −9.1 kcal/mol, indicating the strongest interaction. Using Lineweaver-Burk plot, the results showed a competitive inhibition, hence, it was justified that M. calabura has the potential to manage diabetes and other diseases related to free radicals.

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Antifungal Activity of Essential Oil Extracted from Melaleuca alternifolia Against Pathogenic Fungi on Mango (Mangifera Indica L.) for Mango Postharvest Application

2022-01-01 , Ahmad Anas Nagoor Gunny , Seminai J.J.A. , Mohd Hishamuddin Che Mat , Ahmad A.A. , Yusof R. , Fadhilnor Abdullah

An infection by pathogenic fungi is one of the major problems in post-harvest stage of mangoes. Therefore, the extraction of essential oil from Melaleuca alternifolia is being tested as a natural fungicide for controlling fungus infections of selected mangoes locally known as susu mango. The extraction was performed using Solvent-Free Microwave Assisted Extraction with a yield of 0.57% concentration. The inhibitory effect of this essential oil against isolated fungi from mango was investigated through in-vitro and in-vivo analyses. Distilled water was used as a negative control while chemical fungicide (Globus 5.5) was used as a positive control in the analyses. The mycelial growth inhibition of the extracted essential oil for poisoned food test and disc diffusion method showed 62.50 ± 0.49% and 59.70 ± 0.30%, respectively, for in-vivo experiment which used unwounded and artificially wounded mangoes. The result also demonstrated that the essential oil applied on the mangoes could decrease the disease incidence from 100% to 61.33% for up to 10 days incubation at room temperature compared to that of the control. Hence, the essential oil of Melaleuca alternifolia can act as a green fungicide and is also a promising alternative to the synthetic chemical fungicide for controlling post-harvest disease on mangoes.

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