Johan Ariff Mohtar
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Preferred name
Johan Ariff Mohtar
Official Name
Mohtar, Johan Ariff
Alternative Name
Mohtar, J. A.
Mohtar, Johan Ariff
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Scopus Author ID
56119103200
Researcher ID
FMF-3163-2022

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  • Publication
    Laboratory breeding and rearing of cellar spider, Crossopriza lyoni Blackwall
    (Springer Science and Business Media Deutschland GmbH, 2022-12-01)
    Johan Ariff Mohtar
    ;
    Mohd Faidz Mohamad Shahimin
    Spiders have emerged as one of the leading model organisms in many research fields due to their compelling biology. Often, scientific investigations involving the use of spiders face inevitable problems associated with the lack of specimens from laboratory stock, resulting in difficulties in yielding reproducible investigations for predictive research. Thus, several species of well-studied spiders, including Parasteatoda tepidariorum, have been successfully bred for such purposes. Crossopriza lyoni is a Haplogyne spider, globally distributed and widespread in human inhabitants, prompting interest in various studies over the last decades. Despite its scientific importance, no laboratory-bred C. lyoni has been documented. Therefore, we describe a successful captive breeding system of the species under controlled conditions to establish a laboratory stock culture. Methods for mating induction, egg collection and segregation, artificial embryo incubation, and colony husbandry are discussed. The technique presented is a simple and low-cost approach that is reliable for C. lyoni propagation in the laboratory over several generations. Graphical abstract: [Figure not available: see fulltext.]
      7  3
  • Publication
    Induction of Apoptosis of Melanoma Skin Cancer Cells by Atmospheric Plasma Jet
    ( 2023-01-01)
    Abdullah Z.
    ;
    Siti Khadijah Za'aba
    ;
    Mohammad Taufiq Mustaffa
    ;
    Saidin N.A.
    ;
    Johan Ariff Mohtar
    The apoptotic effect is an important issue in cancer treatment. To achieve this goal, an atmospheric plasma jet (APJ) was set up for use on cultured cells in a temperature-controlled environment. Melanoma skin cancer and normal skin cells were targeted with this device. Following a 5 s plasma exposure, there was a 67% cell death in melanoma skin cancer cells compared with 5% in normal skin cells as measured after 24 h. When the treatment time was increased to 15, a 98% cell death was reported for melanoma skin cancer cells, which was 80% greater than the cell death in normal skin cells. Our observations further indicate that this preferential cell death is largely due to apoptosis. It shows that an APJ is a selective device in the induction of apoptosis in cancer and normal cells. APJ was shown to affect cells directly and indirectly through a plasma-activated medium (PAM). In direct treatment, cells were exposed to plasma while suspended in a culture medium, and in indirect treatment, cells were added to a culture medium previously acti-vated by plasma treatment. PAM was able to induce cell death 29% higher than direct treatment as measured after 48 h. The depth of the growth medium is also one of the factors in the induction of apoptosis of cancer cells. The growth medium protected the cells from plasma exposure. The result shows that the low level (0 mm) of growth medium will cause more cell death as compared with the high level (2 mm) of growth medium. Apoptotic behavior of skin cancer cells was de-duced from the fact that treated cells initially grew and died 12 h following the treatment, while untreated cells continued to grow and proliferate.
      1  38