Iffah Nadhirah Madzuki
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Preferred name
Iffah Nadhirah Madzuki
Official Name
Madzuki, Iffah Nadhirah
Alternative Name
Madzuki, I. N.
Madzuki, Iffah Nadhira
Main Affiliation
Scopus Author ID
57200690177
Researcher ID
DXR-1101-2022

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  • Publication
    Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model
    ( 2021-09-01)
    Ishak N.I.M.
    ;
    Mohamed S.
    ;
    Iffah Nadhirah Madzuki
    ;
    Mustapha N.M.
    ;
    Esa N.M.
    Inflammation and compromised immune responses often increase colorectal cancer (CRC) risk. The immune-modulating effects of limonin on carcinogen/inflammation-induced colorectal cancer (CRC) were studied in mice. Male Balb/c mice were randomly assorted into three groups (n = 6): healthy control, non-treated CRC-induced (azoxymethane/dextran-sulfate-sodium AOM/DSS) control, and CRC-induced + 50 mg limonin/kg body weight. The CRC developments were monitored via macroscopic, histopathological, ELISA, and mRNA expression analyses. Limonin downregulated inflammation (TNF-α, tumor necrosis factor-α), enhanced the adaptive immune responses (CD8, CD4, and CD19), and upregulated antioxidant defense (Nrf2, SOD2) mRNA expressions. Limonin reduced serum malondialdehyde (MDA, lipid peroxidation biomarker), prostaglandin E2, and histopathology inflammation scores, while increasing reduced glutathione (GSH) in CRC-induced mice. Limonin significantly (p < 0.05) increased T cells (CD4 and CD8) and B cells (CD19) in spleen tissues. The CD335 (natural killer cells) were increased in the CRC-induced mice and limonin treatment restored them to normal levels suggesting reinstatement to normal colon conditions. Limonin apparently mitigated CRC development, by ameliorating adaptive immune responses (CD8, CD4, and CD19), reducing inflammation (serum prostaglandin E2; TNF-α, innate immune responses) and oxidative stress, and enhancing the endogenous anti-oxidation defense reactions (GSH) in CRC-induced mice.