Mohamad Fahrurrazi Tompang
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Preferred name
Mohamad Fahrurrazi Tompang
Official Name
Mohamad Fahrurrazi, Tompang
Alternative Name
Fahrurrazi Tompang, Mohamad Fahrurrazi
Tompang, Mohamad Fahrurrazi
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Scopus Author ID
56527099200
Researcher ID
GGZ-1964-2022

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Isolation and characterization of laccase secreting microorganism from Gua Kelam 2, Perlis

2023 , Natasha Yasmin Hasnol Azahari , Nor Anis Syafiqah Suhaily , Sandrasekaran Naresh , Mohamad Fahrurrazi Tompang , Kunasundari Balakrishnan

The current study, involves isolation and characterization of laccase producing microbes from Gua Kelam 2, Perlis, Malaysia. In our study, all isolates were cultivated on nutrient agar containing guaiacol. Only one isolate (GK1) was detected as a positive laccase producer which was then subjected for growth curve and kinetic analysis using nutrient broth and minimal salt medium. For nutrient broth, short exponential phase was recorded from 2nd h until 4th h in contrast to minimal salt medium that exhibited exponential phase from 8th h until 24th h. The highest laccase activity was recorded in nutrient broth (4.50 U L-1) at 4th h incubation compared to minimal salt medium (3.72 U L-1) at 30 h. The isolate GK1, was characterized to be rod shape and Gram positive bacterium. Based on the 16S rRNA sequencing, the isolate determined to be Bacillus subtilis.

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Microbiological removal of hydrogen sulphide from natural rubber latex processing wastewater by Acidithiobacillus thiooxidans strain UniMAP-AIN01

2020 , Sandrasekaran Naresh , Nurul Ain Harmiza Abdullah , Nor Helya Iman Kamaludin , Muhd Afiq Hizami Abdullah , Mohamad Fahrurrazi Tompang

Acidithiobacillus thiooxidans is an acidophilic chemoautotrophic bacterium which capable to convert the toxic hydrogen sulphide in wastewater into non-toxic compounds. The Acidithiobacillus thiooxidans strain UniMAP-AIN01 was previously isolated from a local natural rubber latex processing mill. In this study, the A. thiooxidans strain UniMAP-AIN01 was subjected to its growth performance evaluation in the wastewater of natural rubber latex. The sulphur content in the wastewater was utilized as energy source and support their growth. It was discovered that the A. thiooxidans strain UniMAP-AIN01 has long doubling time of 2 days in thiosulphate medium, while log phase lasted until 6th day of incubation. Regression analysis of the growth kinetic was established with the aid of POLYMATH software. The precision value obtained as follows; linear regression, R2 of 0.9811, adjusted linear regression, Adj R2 of 0.9764, root mean square deviation, RMSD of 0.0015, and variance of 1.992 × 10-5 indicate the data is highly correlated and error is insignificant. Assessment on hydrogen sulphide removal efficiency using locally isolated A. thiooxidans strain UniMAP-AIN01 revealed 90% of hydrogen sulphide removal was achieved within a week.

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