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Improvising non-uniform illumination and low contrast images of soil transmitted helminths image using contrast enhancement techniques

2021-01-01 , Norhanis Ayunie Ahmad Khairudin , Aimi Salihah Abdul Nasir , Lim Chee Chin , Haryati Jaafar , Mohamed Z.

Image enhancement plays an important role in image processing and computer vision. It is used to enhance the visual appearance in an image and also to convert the image suited to the requirement needed for image processing. In this paper, image enhancement is used to produce a better image by enhancing the image quality and highlighting the morphological features of the helminth eggs. Result obtained from enhancement is prepared for segmentation and classification process. The helminth eggs used in this paper are Ascaris Lumbricoides Ova (ALO) and Trichuris Trichiura Ova (TTO). In this study, several enhancement techniques have been performed on 100 images of ALO and TTO which have been captured under three different illuminations: normal, under-exposed and over-exposed images. The techniques used are global contrast stretching, limit contrast, linear contrast stretching, modified global contrast stretching, modified linear contrast stretching, partial contrast and reduce haze. Based on results obtained from these techniques, modified linear contrast stretching and modified global contrast stretching are able to equalize the lighting in the non-uniform illumination images of helminth eggs. Both techniques are suitable to be used on non-uniform illumination images and also able to improve the contrast in the image without affecting or removing the key features in ALO and TTO images as compared to the other techniques. Hence, the resultant images would become useful for parasitologist in analyzing helminth eggs.

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Image segmentation using k-means clustering and otsu's thresholding with classification method for human intestinal parasites

2020-07-09 , Khairudin Norhanis Ayunie Ahmad , Rohaizad Nurfatin Shamimi , Aimi Salihah Abdul Nasir , Lim Chee Chin , Haryati Jaafar , Mohamed Z.

Helminth is one of the intestinal parasites that may cause harm and death to human. It is very important to have a system that is capable of assisting the technologist in investigating of fecal samples. In this paper, an automatic classification process is proposed to detect the different types of helminth eggs from fecal samples by using image processing technique. 50 samples of Ascaris Lumbricoides Ova (ALO) and Trichuris Trichiura Ova (TTO) are tested. First, these images undergo partial contrast stretching (PCS) technique to enhance the target images. Next, RGB and HSV color model have been compared in order to identify which color component is able to ease the segmentation process. S component shows a good results with high contrast between the target and the unwanted region. Then, Otsu's thresholding and k-means clustering are compared in order to to select the most suitable image processing method to be used in classification procedure. k-means clustering shows a better results compared to Otsu's thresholding. In classification process, area and size have been chosen as the feature to extract for the classification. The ratio for successfully detected ALO species is 84% while TTO is 76%.

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Fast k-means clustering algorithm for malaria detection in thick blood smear

2020-11-09 , Aris T.A. , Aimi Salihah Abdul Nasir , Lim Chee Chin , Haryati Jaafar , Mohamed Z.

Lots of people all over the world is threaten by a popular blood infection illness that is called as malaria. According to this fact, immediate diagnosis tests are essential to avoid the malaria parasites from expanding in every part of the body. Malaria detection is based on parasitic count process on thick blood smear samples. Anyhow, this mechanism consist the chances of misinterpretation of parasites on behalf to human flaws. Thus, this research objective is to investigate the segmentation performance for improving malaria detection in thick blood smear images through fast k-means clustering algorithm on various color models. In this research, fast kmeans clustering is used because of its advantage which is no need to retrain cluster center that causes time taken to train the image cluster centers is reduce. Meanwhile, different color models have been utilized in order to identify the most relevant color model that obviously highlight the parasites. Five varied color models namely RGB, XYZ, HSV, YUV and CMY are selected and 15 color components namely R, G, B, X, Y, Z, H, S, V, Y, U, V, C, M and Y component have been derived with the aim to discover which color component is the topnotch for malaria parasites detection. In general, around 100 thick blood smear images have been tested in this study and the outcomes reveal that the best segmentation performance is segmentation through R component of RGB with 99.81% accuracy.

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A fast and efficient segmentation of soil-transmitted helminths through various color models and k-means clustering

2021-01-01 , Norhanis Ayunie Ahmad Khairudin , Aimi Salihah Abdul Nasir , Lim Chee Chin , Haryati Jaafar , Mohamed Z.

Soil-transmitted helminths (STH) are one of the causes of health problems in children and adults. Based on a large number of helminthiases cases that have been diagnosed, a productive system is required for the identification and classification of STH in ensuring the health of the people is guaranteed. This paper presents a fast and efficient method to segment two types of STH; Ascaris Lumbricoides Ova (ALO) and Trichuris Trichiura Ova (TTO) based on the analysis of various color models. Firstly, the ALO and TTO images are enhanced using modified global contrast stretching (MGCS) technique, followed by the extraction of color components from various color models. In this study, segmentation based on various color models such as RGB, HSV, L*a*b and NSTC have been used to identify, simplify and extract the particular color needed. Then, k-means clustering is used to segment the color component images into three clusters region which are target (helminth eggs), unwanted and background regions. Then, additional processing steps are applied on the segmented images to remove the unwanted region from the images and to restore the information of the images. The proposed techniques have been evaluated on 100 images of ALO and TTO. Results obtained show saturation component of HSV color model is the most suitable color component to be used with the k-means clustering technique on ALO and TTO images which achieve segmentation performance of 99.06% for accuracy, 99.31% for specificity and 95.06% for sensitivity.

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Color constancy analysis approach for color standardization on malaria thick and thin blood smear images

2021-01-01 , Thaqifah Ahmad Aris , Aimi Salihah Abdul Nasir , Haryati Jaafar , Lim Chee Chin , Mohamed Z.

Malaria is an extensively prevalent blood infection, the most severe and widespread parasitic disease that stirring millions of people in the world. Currently, microscopy diagnosis still the most widely used method for malaria diagnosis. However, this procedure contains the probability of miscalculation of parasites due to human error. Computerized system by using image processing is recognized as a quick and easy ways to analyze a lot of blood samples. However, because of the non-standard preparation of the blood slides which producing color varieties in different slides will result on low quality images. Hence, it is difficult to identify the existence of malaria parasites as well as observing its morphological characteristics to recognize malaria parasites. Therefore, this paper aims to analyze the standardization performance between six types of color constancy algorithms namely, gray world (GW), white patch (WP), modified white patch (MWP), progressive hybrid (PH), shades of gray (SoG) and gray edge (GE) on both thick and thin blood smear malaria images of P. falciparum and P. vivax species. Six types of color constancy algorithms standardization performance are analysed by using quantitative measure namely, peak signal to noise ratio (PSNR), normalized absolute error (NAE), mean square error (MSE) and root mean square error (RMSE). Based on the qualitative and quantitative findings, the results show that SoG algorithm is the best color constancy as compared to others proposed color constancy. SoG algorithm has achieved the highest PSNR and lowest NAE, MSE and RMSE values, thus proved that the quality of malaria images have been improved.

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Robust Image Processing Framework for Intelligent Multi-Stage Malaria Parasite Recognition of Thick and Thin Smear Images

2023-02-01 , Aris T.A. , Aimi Salihah Abdul Nasir , Wan Azani Wan Mustafa , Mohd Yusoff Mashor , Haryanto E.V. , Mohamed Z.

Malaria is a pressing medical issue in tropical and subtropical regions. Currently, the manual microscopic examination remains the gold standard malaria diagnosis method. Nevertheless, this procedure required highly skilled lab technicians to prepare and examine the slides. Therefore, a framework encompassing image processing and machine learning is proposed due to inconsistencies in manual inspection, counting, and staging. Here, a standardized segmentation framework utilizing thresholding and clustering is developed to segment parasites’ stages of P. falciparum and P. vivax species. Moreover, a multi-stage classifier is designed for recognizing parasite species and staging in both species. Experimental results indicate the effectiveness of segmenting thick smear images based on Phansalkar thresholding garnered an accuracy of 99.86%. The employment of variance and new transferring process for the clustered members, enhanced k-means (EKM) clustering has successfully segmented all malaria stages with accuracy and an F1-score of 99.20% and 0.9033, respectively. In addition, the accuracies of parasite detection, species recognition, and staging obtained through a random forest (RF) accounted for 86.89%, 98.82%, and 90.78%, respectively, simultaneously. The proposed framework enables versatile malaria parasite detection and staging with an interactive result, paving the path for future improvements by utilizing the proposed framework on all others malaria species.