Ahmad Anas Nagoor Gunny
Thumbnail Image
Preferred name
Ahmad Anas Nagoor Gunny
Official Name
Ahmad Anas, Nagoor Gunny
Alternative Name
Nagoor Gunny, Ahmad Anas
Gunny, A. A.N.
Nagoor Gunny, A. A.
Ahmad Anas, N. G.
Gunny, Ahmad Anas Nagoor
Main Affiliation
Scopus Author ID
55940833700
Researcher ID
AFF-6251-2022

Research Output

Filters

2 1
2
2
Reset filters

Settings
Now showing 1 - 2 of 2
Thumbnail Image
Publication

Investigation of the white-rot fungus biomass extraction conditions and their impact on phenolic, flavonoids content and antioxidant activities

2023-07 , Ahmad Anas Nagoor Gunny , Shahizatul Emilia Jahidin , Zulkarnain Mohamed Idris , Gidado Muhammad Junaid , Safa Senan Mahmod , Norhidayah Bohari

White-rot fungus are known as natural source of antioxidant compounds such as flavonoids, phenolic, and other enzymes through extraction processes. In this study, Phanerochaete chrysosporium and Panus tigrinus biomass were subjected into different extraction conditions to determine the best optimal level for the extraction of phenolic and flavonoids compounds. P. chrysosporium produced high yield of phenolic (0.7041 mg/mL) and flavonoids (0.0286 mg/mL) compared to P. tigrinus; 0.3755 mg/mL and 0.0109 mg/mL of phenolic and flavonoids compounds, respectively. P. chrysosporium has higher antioxidant activity (70.31%) compared with P. tigrinus (25.30%). Therefore, P. chrysosporium was selected to determine the optimum condition for extraction method of phenolic and flavonoids compounds using aquoues-two phase system. The conditions used in the extraction was time within the range of 30 min to 180 min and temperature range from 250°C to 650°C. The conditions were optimized using Response Surface Method (RSM) under Central Composite Design. The best optimum condition was 105 min and 450°C, this condition produced the highest concentrations of phenolic, flavonoids, and antioxidant activity (AA%) at 0.4269 mg/mL, 0.0695 mg/mL, and 76.13% respectively. Thin Layer Chromatography (TLC) was used to determine the presence of phenolic and flavonoids in the sample. The Rf value from P. chrysosporium was 0.98 closed to the Rf values of gallic acid and 3,4-hydroxybenzoic acid at 0.95 and 0.97, respectively. Fourier Transform Infrared (FTIR) spectroscopy shows that P. chrysosporium have gallic acid, 3,4 hydroxybenzoic acid and small amount of quercetin.

View
Thumbnail Image
Publication

Antioxidant and xanthine oxidase inhibitory activity of sesbania grandiflora extract

2024-07 , Ahmad Anas Nagoor Gunny , Ahmad Iqrak Azri , Mohd Hishamuddin Che Mat , Syaheerah Syazana Syalabiah Salim , Mohamed Mydin Hj M.Abdul Kader

Xanthine oxidase is a crucial enzyme in the regulation of uric acid, with elevated levels leading to gout and hyperuricemia. To combat these conditions, a synergistic approach using antioxidants and xanthine oxidase inhibitors presents a promising treatment strategy. Given the side effects of synthetic inhibitors, this study explores the natural xanthine oxidase inhibitory and antioxidant properties of Sesbania grandiflora leaves extract. The analysis revealed a significant free radical inhibition rate of 61.59 ± 0.90% at a concentration of 100 μg/mL. The SC₅₀ value for the leaves extract was 16.59 ± 0.67 μg/mL, compared to 7.76 ± 0.23 μg/mL for gallic acid, a positive control. The leaves extract also demonstrated notable efficiency in inhibiting uric acid formation, with an IC₅₀ value of 0.36 ± 0.341 μg/mL, although this was higher than the 0.01 ± 0.005 μg/mL value for allopurinol. A strong correlation (r = 0.9958) was found between the antioxidant activity and xanthine oxidase inhibition of the extract, underscoring its effectiveness. This study is the first to report the combined effects of xanthine oxidase inhibition and antioxidant activity in Sesbania grandiflora, highlighting its potential as a natural treatment for gout and related diseases.

View