Adilah Ayoib
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Preferred name
Adilah Ayoib
Official Name
Ayoib, Adilah
Alternative Name
Ayoib, Adilah
Ayoib, A.
Main Affiliation
Scopus Author ID
57186613000
Researcher ID
GWV-4156-2022

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  • Publication
    Distinct Detection of Ganoderma Boninense On Metal Oxides-Gold Nanoparticle Composite Deposited Interdigitated Electrode DNA sensor
    ( 2021-12-14)
    Thivina V.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Adilah Ayoib
    ;
    Nordin N.K.S.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    Oil palms suffer severe losses due to Ganoderma boninense infection that causes Basal Stem Rot (BSR). The available detection measuring the severity of BSR disease have not proved satisfactory output. Due to the influence of oil palm industry in country's economy, effective and efficient means of diagnostic measure is mandatory. Among the available diagnostic tools, biosensors were redeemed to yield the most rapid and selective results. To overcome the current issues, herein Interdigitated Electrode (IDE) electrochemical DNA biosensor to detect Ganoderma boninense was successfully designed and fabricated by thermal deposition. Lift-off photolithography fabrication process was applied followed by the surface chemical functionalization via seed deposition. Zinc Oxide (ZnO) and Titanium Dioxide (TiO2) were overlaid and the functionalized metal oxides IDE surfaces were used to detect DNA sequence complementation from Ganoderma boninense. Furthermore, gold nanoparticles were doped to increase the surface to volume ratio and enhance biocompatibility. Characterizations were made by validating the sensor's topology characteristics and electrical characteristics. From the results recorded, it has been justified that IDE with ZnO doped with gold nanoparticles surface serves as an excellent DNA sensor for the detection of Ganoderma boninense with a remarkable current of 290 nA and 176 nA for immobilization and hybridization respectively.
  • Publication
    Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application
    ( 2024-06-01)
    Adilah Ayoib
    ;
    Karim N.A.A.A.
    ;
    Uda Hashim
    ;
    Shahidah Arina Shamsuddin
    ;
    Abd Rahman S.F.
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nor Azizah Parmin
    Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 µm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.
  • Publication
    Automated, high-throughput DNA extraction protocol for disposable label free, microfluidics integrating DNA biosensor for oil palm pathogen, Ganoderma boninense
    ( 2020-05-01)
    Adilah Ayoib
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    Basal Stem Rot and Upper Stem Rot diseases caused by pathogenic fungus Ganoderma boninense continue to be a major plight in the palm oil industry. Despite continuous research in combating the problem, resolution remains stagnant. Here, developed an automated, high-throughput DNA extraction protocol on microfluidics device for a quick, disposable, label-free detection, within 2 h of assessment. Microfluidics was designed using AutoCAD software, fabricated on microscopic glass substrate using negative photoresist (SU-8 2015) and molded with a biopolymer silicone, Polydimethylsiloxane. G. boninense and unknown pathogenic fungus isolated from rotten mushroom were grown and fractions of extracted DNA were pooled and analyzed for comparison along with synthetic ssDNA of G. boninense. Results from LPM and HPM show successful fabrication with ≤0.1 mm variance between the dimensions in the design before and after lithography process. The PDMS microfluidics show no leakage when run with DNA samples. Analyses from I-V measurement, UV–vis, FTIR, and PCR show comparable results between extracted and synthetic ssDNA of G. boninense and a contrast with the unknown pathogenic fungus, indicating a successful DNA extraction protocol via microfluidics for label-free identification of G. boninense. Optimization of DNA extraction can be further devised for applicability on lab-on-a-chip devices.
      2  2
  • Publication
    Cost-effective fabrication of polydimethylsiloxane (PDMS) microfluidics for point-of-care application
    ( 2024-06)
    Adilah Ayoib
    ;
    Noor Amalina Aini Abdul Karim
    ;
    Uda Hashim
    ;
    Shahidah Arina Shamsuddin
    ;
    Siti Fatimah Abd Rahman
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nor Azizah Parmin
    Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 μm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.
      23  1
  • Publication
    Gold-nanoparticle associated deep eutectic solution mediates early bio detection of ovarian cancer
    ( 2025-01)
    S. Uvambighai Devi
    ;
    Nor Azizah Parmin
    ;
    N. Fareezah Jaapar
    ;
    F. Syakirah Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Muhammad Nur Aiman Uda
    ;
    Voon Chun Hong
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Adilah Ayoib
    ;
    Shahidah Arina Shamsuddin
    ;
    Norizah Abd Karim
    Gold nanoparticles (AuNPs) have indeed been extensively researched in biological and photothermal therapy applications in recent years. This study aims to enhance the sensitivity of biosensors for early detection of ovarian cancer biomarkers by investigating the efficacy of DES-mediated surface functionalization of AuNPs. Additionally, the impact of DES on the stability and dispersion of AuNPs on SiO2 support is assessed to optimize sensor performance. A simple DES-mediated synthesis method for efficient amine surface functionalization of silicon dioxide (SiO2) to incorporate tiny AuNPs for antibody biosensors. Physical characterization [Scanning Electron Microscope (SEM), Ultraviolet-Visible Spectrophotometer (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR), and 3D Profiler] and electrical characterization (Keithley) have been done to determine the functionalization of the modified IDE surface. SEM analysis indicated the resultant nanoparticles have truncated spherical shapes. There is just a peak recorded by UV-Vis at 504-540 nm with AuNPs due to the formation of monodispersed AuNPs. When the conjugation of DES with samples is measured, the curves are identical in form, and the highest peak after conjugation has remained at 230 nm but the SPR absorption peak becomes narrower and moves toward greater wavelengths, indicating the conjugation between the molecules. Furthermore, when the DES is conjugated with AuNPs, 3-Aminopropyltriethoxysilane (APTES), antibody, and protein, the peaks gradually increased and became narrower, where O-H at 3280 cm-1, C-H at 2809 cm-1 and 2933 cm-1, CH2 at 1448 cm-1, CH3 at 1268 cm-1, C-OH at 1048 cm-1 and 1110 cm-1 and C-N+ at 844 cm-1 as analyzed by FTIR. Moreover, it can be observed that the 3D profilometer revealed a few red-colored areas, which are the portion that protrudes from the IDE surface. Based on the findings, it is possible to infer that this immunosensor does have the prospective to be used in clinical investigations for the precise detection of ovarian cancer or other biomarkers. The capacitance, transmittance, and resistivity profiles of the biosensor clearly distinguished between the antibody immobilization and the affinity binding. The presence of a DES-mediated synthetic approach increased the possibility of supporting different metal nanoparticles on SiO2 as the potential platform for biosensor applications.
      22  2
  • Publication
    Automated, high-throughput DNA extraction protocol for disposable label free, microfluidics integrating DNA biosensor for oil palm pathogen, Ganoderma boninense
    ( 2020-05-01)
    Adilah Ayoib
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    Basal Stem Rot and Upper Stem Rot diseases caused by pathogenic fungus Ganoderma boninense continue to be a major plight in the palm oil industry. Despite continuous research in combating the problem, resolution remains stagnant. Here, developed an automated, high-throughput DNA extraction protocol on microfluidics device for a quick, disposable, label-free detection, within 2 h of assessment. Microfluidics was designed using AutoCAD software, fabricated on microscopic glass substrate using negative photoresist (SU-8 2015) and molded with a biopolymer silicone, Polydimethylsiloxane. G. boninense and unknown pathogenic fungus isolated from rotten mushroom were grown and fractions of extracted DNA were pooled and analyzed for comparison along with synthetic ssDNA of G. boninense. Results from LPM and HPM show successful fabrication with ≤0.1 mm variance between the dimensions in the design before and after lithography process. The PDMS microfluidics show no leakage when run with DNA samples. Analyses from I-V measurement, UV–vis, FTIR, and PCR show comparable results between extracted and synthetic ssDNA of G. boninense and a contrast with the unknown pathogenic fungus, indicating a successful DNA extraction protocol via microfluidics for label-free identification of G. boninense. Optimization of DNA extraction can be further devised for applicability on lab-on-a-chip devices.
      33  7