Noor Hasyierah Mohd Salleh
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Noor Hasyierah Mohd Salleh
Official Name
Noor Hasyierah, Mohd Salleh
Alternative Name
Salleh, N. H.M.
Salleh, N. H.Mohd
Mohd Salleh, N. H.
Mohd Salleh, Noor Hasyierah
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Scopus Author ID
41762655600

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Inhibition of Aspergillus flavus by Momordica charantia Ethanolic Leaves Extract for Mushroom Protection

2023-06-09 , Noor Hasyierah Mohd Salleh , Zulkarnay Zakaria , Aziz N.H. , Ahmad Anas Nagoor Gunny , Alina Rahayu Mohamed , Munusamy T.

Pleurotus ostreatus, better known as oyster mushroom is one of the world’s widely cultivated mushroom. Infection from pathogenic fungi is one of the major reasons causing losses of mushroom during cultivation. The objective of this study is to investigate the antifungal property of Momordica charantia leaves extract against Aspergillus flavus for mushroom protection. The inhibitory effect of M. charantia leaves extract against A. flavus isolated from infected P. ostreatus mushroom bag was investigated using pour plate technique. Inhibition of A. flavus mycelial growth was the highest at extract concentration of 9.64 v/v %, temperature 26℃ and incubation time of 96 hours with inhibitory value of 70.58 ± 0.00 %, 78.4 ± 0.00 % and 63.33 ± 2.89 % respectively. Structural morphology of the mycelia grown on the treated plate also showed morphological alteration compared to the control plate. Hence, Momordica charantia leaves extract may serves as a promising fungicides alternative to chemical use for controlling invasion of A. flavus on oyster mushroom.

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Valorization of Momordica charantia seeds into phytogenically synthesized silver nanoparticles for the protection of oyster mushrooms against Trichoderma pleuroticola

2023-12-01 , Norhidayah Abd Aziz , Noor Hasyierah Mohd Salleh , Subash Chandra Bose Gopinath , Kamaruddin A.H. , Muhammad Izzat Ridzuan , Ahmad N.A.

Green mold caused by Trichoderma pleuroticola is the most invasive disease of oyster mushrooms, causing massive losses worldwide. Herein, we valorized food waste, the Momordica seed extract (MSE), into silver nanoparticles (AgNPs-MSE). The synthesis parameters were optimized, and the biogenic AgNPs showed a remarkable antifungal activity against T. pleuroticola. Quantitative and qualitative analyses showed that the MSE was rich in bioactive compounds. The highly stable AgNPs-MSE was synthesized under the following conditions: MSE–AgNO3 ratio, 5:95; synthesis time, 9 min; temperature, 60 °C; and pH, 8.0. The UV absorption spectra showed surface plasma resonance peak at 428 nm, confirming the presence of spherical nanoparticles. FTIR spectra ascertained the involvement of phytoconstituents in the MSE during synthesis, and X-ray diffraction analysis revealed a face-centered cubic (fcc) crystalline structure with an average particle size of 11.34 nm. HR-TEM analysis corroborated the spherical shape, with a nanoparticle size of 8.0 nm. EDX analysis revealed the presence of silver, carbon, oxygen, nitrogen, sulfur, and chlorine in the AgNPs-MSE, which was further substantiated by XPS analysis. A zeta potential of − 29.7 mV and PDI of 0.144 revealed the good stability and uniformity of the synthesized AgNPs-MSE. The optimized AgNPs-MSE exhibited a potent antifungal activity against T. pleuroticola at 300 ppm in vitro. SEM and TEM analyses of AgNPs-MSE-treated T. pleuroticola showed hyphal system breakage and leakage of cytoplasmic contents that leads to cell death. Therefore, the use of the AgNPs-MSE as a nanoparticle-based fungicide has the potential to effectively control T. pleuroticola for oyster mushroom protection.

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